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Optical Gratings Coated with Thin Si3N4 Layer for Efficient Immunosensing by Optical Waveguide Lightmode Spectroscopy.

Diéguez L, Caballero D, Calderer J, Moreno M, Martínez E, Samitier J - Biosensors (Basel) (2012)

Bottom Line: A thin layer of 10 nm of transparent silicon nitride was deposited on commercial optical gratings by means of sputtering.The quality of the layer was tested by x-ray photoelectron spectroscopy and atomic force microscopy.The potential of the Si3N4 as functional layer in a real-time biosensor opens new ways for the integration of optical waveguides with microelectronics.

View Article: PubMed Central - PubMed

Affiliation: Department of Electronics, University of Barcelona, C/Martí i Franquès 1, Barcelona, ES 08028, Spain. lorena.dieguez@unisa.edu.au.

ABSTRACT
New silicon nitride coated optical gratings were tested by means of Optical Waveguide Lightmode Spectroscopy (OWLS). A thin layer of 10 nm of transparent silicon nitride was deposited on commercial optical gratings by means of sputtering. The quality of the layer was tested by x-ray photoelectron spectroscopy and atomic force microscopy. As a proof of concept, the sensors were successfully tested with OWLS by monitoring the concentration dependence on the detection of an antibody-protein pair. The potential of the Si3N4 as functional layer in a real-time biosensor opens new ways for the integration of optical waveguides with microelectronics.

No MeSH data available.


Related in: MedlinePlus

Relationship between the adsorbed mass of HSA protein in the surface and the HSA solution concentration. The dash line shows a linear fit for the dynamic range of the sensor.
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biosensors-02-00114-f007: Relationship between the adsorbed mass of HSA protein in the surface and the HSA solution concentration. The dash line shows a linear fit for the dynamic range of the sensor.

Mentions: The relationship between the adsorbed mass, attributed to the HSA protein and the protein concentration plotted in a semi-logarithmic scale is shown in Figure 7. The experimental mass values were fitted using a sigmoidal fit with a correlation factor of R2 = 0.9969. The graphic also suggests a linear fit within the range of 10−3 M to 10−6 M with a R2 value of 0.99689. The sensitivity of the system within the dynamic range is 34 ng/cm2 for the HSA detection.


Optical Gratings Coated with Thin Si3N4 Layer for Efficient Immunosensing by Optical Waveguide Lightmode Spectroscopy.

Diéguez L, Caballero D, Calderer J, Moreno M, Martínez E, Samitier J - Biosensors (Basel) (2012)

Relationship between the adsorbed mass of HSA protein in the surface and the HSA solution concentration. The dash line shows a linear fit for the dynamic range of the sensor.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263575&req=5

biosensors-02-00114-f007: Relationship between the adsorbed mass of HSA protein in the surface and the HSA solution concentration. The dash line shows a linear fit for the dynamic range of the sensor.
Mentions: The relationship between the adsorbed mass, attributed to the HSA protein and the protein concentration plotted in a semi-logarithmic scale is shown in Figure 7. The experimental mass values were fitted using a sigmoidal fit with a correlation factor of R2 = 0.9969. The graphic also suggests a linear fit within the range of 10−3 M to 10−6 M with a R2 value of 0.99689. The sensitivity of the system within the dynamic range is 34 ng/cm2 for the HSA detection.

Bottom Line: A thin layer of 10 nm of transparent silicon nitride was deposited on commercial optical gratings by means of sputtering.The quality of the layer was tested by x-ray photoelectron spectroscopy and atomic force microscopy.The potential of the Si3N4 as functional layer in a real-time biosensor opens new ways for the integration of optical waveguides with microelectronics.

View Article: PubMed Central - PubMed

Affiliation: Department of Electronics, University of Barcelona, C/Martí i Franquès 1, Barcelona, ES 08028, Spain. lorena.dieguez@unisa.edu.au.

ABSTRACT
New silicon nitride coated optical gratings were tested by means of Optical Waveguide Lightmode Spectroscopy (OWLS). A thin layer of 10 nm of transparent silicon nitride was deposited on commercial optical gratings by means of sputtering. The quality of the layer was tested by x-ray photoelectron spectroscopy and atomic force microscopy. As a proof of concept, the sensors were successfully tested with OWLS by monitoring the concentration dependence on the detection of an antibody-protein pair. The potential of the Si3N4 as functional layer in a real-time biosensor opens new ways for the integration of optical waveguides with microelectronics.

No MeSH data available.


Related in: MedlinePlus