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Development of an electrochemical-based aspartate aminotransferase nanoparticle ir-C biosensor for screening of liver diseases.

Hsueh CJ, Wang JH, Dai L, Liu CC - Biosensors (Basel) (2012)

Bottom Line: Aspartate aminotransaminase (AST) is a hepatocelluar enzyme released into the bloodstream when hepatic cells are damaged, resulting in elevated blood levels of AST.This biosensor is capable of measuring AST levels in a phosphate buffer and undiluted human serum over the concentration range of 0 to 0.89 μg/mL AST concentration (corresponding to 0-250 UL-1 specific activity).The biosensor operates at relatively low oxidation potential (+0.3 volt (V) versus the printed Ag/AgCl), minimizing any potential chemical interference in human serum.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemical Engineering and Electronics Design Center, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA. cxh300@case.edu.

ABSTRACT
Aspartate aminotransaminase (AST) is a hepatocelluar enzyme released into the bloodstream when hepatic cells are damaged, resulting in elevated blood levels of AST. A single use, disposable biosensor prototype, composed of catalytic iridium nano-particles dispersed on carbon paste, was developed to detect enzymatically-produced H2O2 in AST-mediated reactions. This biosensor is capable of measuring AST levels in a phosphate buffer and undiluted human serum over the concentration range of 0 to 0.89 μg/mL AST concentration (corresponding to 0-250 UL-1 specific activity). The biosensor operates at relatively low oxidation potential (+0.3 volt (V) versus the printed Ag/AgCl), minimizing any potential chemical interference in human serum. The measurements of AST in human serum using the biosensor compared well with those measured by standard hospital spectrophotometric assays. This Ir-C biosensor may be useful for AST measurements in the clinical environment.

No MeSH data available.


Comparison of measurements achieved by a Vista 1500 spectrophotometer to the Ir-C sensor response currents of human serum.
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biosensors-02-00234-f007: Comparison of measurements achieved by a Vista 1500 spectrophotometer to the Ir-C sensor response currents of human serum.

Mentions: The quantification of AST in human serum was carried out for potential clinical applications. 100 mM potassium chloride solution was added into an undiluted serum solution as the supportive electrolyte. Figure 6 shows that the amperometric current outputs recorded at the 150th second exhibited a sensitivity, 18.91 nA/[μg/mL] (corresponding to 0.067 nA/UL−1), which was almost identical to that in the phosphate buffer solution. This sensitivity was higher than the experimental results in the QC serum measurement reported by Guo et al. [10]. Serum quantification had the limit of detection LOD = 0.09 μg/mL, corresponding to an AST specific activity of 25.3 UL−1. These quantifications were then compared with the assessments by Spectrophotometry Vista 1500 in the University Hospitals of Cleveland, Ohio. Figure 7 showed that the AST detection by the Ir-C biosensor and the spectrophotometric results, indicating the biosensor prototype, can be useful in detecting AST more than 3-fold of the normal upper limits (around 125 UL−1) in a serum sample. Therefore, the proposed Ir-C biosensor may have clinical utility in in vitro AST quantification in human serum samples.


Development of an electrochemical-based aspartate aminotransferase nanoparticle ir-C biosensor for screening of liver diseases.

Hsueh CJ, Wang JH, Dai L, Liu CC - Biosensors (Basel) (2012)

Comparison of measurements achieved by a Vista 1500 spectrophotometer to the Ir-C sensor response currents of human serum.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263573&req=5

biosensors-02-00234-f007: Comparison of measurements achieved by a Vista 1500 spectrophotometer to the Ir-C sensor response currents of human serum.
Mentions: The quantification of AST in human serum was carried out for potential clinical applications. 100 mM potassium chloride solution was added into an undiluted serum solution as the supportive electrolyte. Figure 6 shows that the amperometric current outputs recorded at the 150th second exhibited a sensitivity, 18.91 nA/[μg/mL] (corresponding to 0.067 nA/UL−1), which was almost identical to that in the phosphate buffer solution. This sensitivity was higher than the experimental results in the QC serum measurement reported by Guo et al. [10]. Serum quantification had the limit of detection LOD = 0.09 μg/mL, corresponding to an AST specific activity of 25.3 UL−1. These quantifications were then compared with the assessments by Spectrophotometry Vista 1500 in the University Hospitals of Cleveland, Ohio. Figure 7 showed that the AST detection by the Ir-C biosensor and the spectrophotometric results, indicating the biosensor prototype, can be useful in detecting AST more than 3-fold of the normal upper limits (around 125 UL−1) in a serum sample. Therefore, the proposed Ir-C biosensor may have clinical utility in in vitro AST quantification in human serum samples.

Bottom Line: Aspartate aminotransaminase (AST) is a hepatocelluar enzyme released into the bloodstream when hepatic cells are damaged, resulting in elevated blood levels of AST.This biosensor is capable of measuring AST levels in a phosphate buffer and undiluted human serum over the concentration range of 0 to 0.89 μg/mL AST concentration (corresponding to 0-250 UL-1 specific activity).The biosensor operates at relatively low oxidation potential (+0.3 volt (V) versus the printed Ag/AgCl), minimizing any potential chemical interference in human serum.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemical Engineering and Electronics Design Center, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA. cxh300@case.edu.

ABSTRACT
Aspartate aminotransaminase (AST) is a hepatocelluar enzyme released into the bloodstream when hepatic cells are damaged, resulting in elevated blood levels of AST. A single use, disposable biosensor prototype, composed of catalytic iridium nano-particles dispersed on carbon paste, was developed to detect enzymatically-produced H2O2 in AST-mediated reactions. This biosensor is capable of measuring AST levels in a phosphate buffer and undiluted human serum over the concentration range of 0 to 0.89 μg/mL AST concentration (corresponding to 0-250 UL-1 specific activity). The biosensor operates at relatively low oxidation potential (+0.3 volt (V) versus the printed Ag/AgCl), minimizing any potential chemical interference in human serum. The measurements of AST in human serum using the biosensor compared well with those measured by standard hospital spectrophotometric assays. This Ir-C biosensor may be useful for AST measurements in the clinical environment.

No MeSH data available.