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Development of an electrochemical-based aspartate aminotransferase nanoparticle ir-C biosensor for screening of liver diseases.

Hsueh CJ, Wang JH, Dai L, Liu CC - Biosensors (Basel) (2012)

Bottom Line: Aspartate aminotransaminase (AST) is a hepatocelluar enzyme released into the bloodstream when hepatic cells are damaged, resulting in elevated blood levels of AST.This biosensor is capable of measuring AST levels in a phosphate buffer and undiluted human serum over the concentration range of 0 to 0.89 μg/mL AST concentration (corresponding to 0-250 UL-1 specific activity).The biosensor operates at relatively low oxidation potential (+0.3 volt (V) versus the printed Ag/AgCl), minimizing any potential chemical interference in human serum.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemical Engineering and Electronics Design Center, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA. cxh300@case.edu.

ABSTRACT
Aspartate aminotransaminase (AST) is a hepatocelluar enzyme released into the bloodstream when hepatic cells are damaged, resulting in elevated blood levels of AST. A single use, disposable biosensor prototype, composed of catalytic iridium nano-particles dispersed on carbon paste, was developed to detect enzymatically-produced H2O2 in AST-mediated reactions. This biosensor is capable of measuring AST levels in a phosphate buffer and undiluted human serum over the concentration range of 0 to 0.89 μg/mL AST concentration (corresponding to 0-250 UL-1 specific activity). The biosensor operates at relatively low oxidation potential (+0.3 volt (V) versus the printed Ag/AgCl), minimizing any potential chemical interference in human serum. The measurements of AST in human serum using the biosensor compared well with those measured by standard hospital spectrophotometric assays. This Ir-C biosensor may be useful for AST measurements in the clinical environment.

No MeSH data available.


Sensor prototype and reaction mechanism used for the AST detection.
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biosensors-02-00234-f001: Sensor prototype and reaction mechanism used for the AST detection.

Mentions: The single-use, disposable sensor platform had an overall dimension of 30 mm × 5.5 mm and was manufactured by a multi-layer approach using screen-printing technology. The detailed fabrication process had been discussed in previous publications [17,20]. Briefly, a 787 mm × 584 mm PET polymer was employed as the substrate, and silver ink was used as electrical contacts. The prepared Ir-C ink was then printed as the working and counter electrode, Ag/AgCl was printed as the reference electrode. The geometric surface area for the printed working electrode was 7.85 × 10−3 cm2. Figure 1 shows the sensor prototype which will be used for AST detection.


Development of an electrochemical-based aspartate aminotransferase nanoparticle ir-C biosensor for screening of liver diseases.

Hsueh CJ, Wang JH, Dai L, Liu CC - Biosensors (Basel) (2012)

Sensor prototype and reaction mechanism used for the AST detection.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263573&req=5

biosensors-02-00234-f001: Sensor prototype and reaction mechanism used for the AST detection.
Mentions: The single-use, disposable sensor platform had an overall dimension of 30 mm × 5.5 mm and was manufactured by a multi-layer approach using screen-printing technology. The detailed fabrication process had been discussed in previous publications [17,20]. Briefly, a 787 mm × 584 mm PET polymer was employed as the substrate, and silver ink was used as electrical contacts. The prepared Ir-C ink was then printed as the working and counter electrode, Ag/AgCl was printed as the reference electrode. The geometric surface area for the printed working electrode was 7.85 × 10−3 cm2. Figure 1 shows the sensor prototype which will be used for AST detection.

Bottom Line: Aspartate aminotransaminase (AST) is a hepatocelluar enzyme released into the bloodstream when hepatic cells are damaged, resulting in elevated blood levels of AST.This biosensor is capable of measuring AST levels in a phosphate buffer and undiluted human serum over the concentration range of 0 to 0.89 μg/mL AST concentration (corresponding to 0-250 UL-1 specific activity).The biosensor operates at relatively low oxidation potential (+0.3 volt (V) versus the printed Ag/AgCl), minimizing any potential chemical interference in human serum.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemical Engineering and Electronics Design Center, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA. cxh300@case.edu.

ABSTRACT
Aspartate aminotransaminase (AST) is a hepatocelluar enzyme released into the bloodstream when hepatic cells are damaged, resulting in elevated blood levels of AST. A single use, disposable biosensor prototype, composed of catalytic iridium nano-particles dispersed on carbon paste, was developed to detect enzymatically-produced H2O2 in AST-mediated reactions. This biosensor is capable of measuring AST levels in a phosphate buffer and undiluted human serum over the concentration range of 0 to 0.89 μg/mL AST concentration (corresponding to 0-250 UL-1 specific activity). The biosensor operates at relatively low oxidation potential (+0.3 volt (V) versus the printed Ag/AgCl), minimizing any potential chemical interference in human serum. The measurements of AST in human serum using the biosensor compared well with those measured by standard hospital spectrophotometric assays. This Ir-C biosensor may be useful for AST measurements in the clinical environment.

No MeSH data available.