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Microfabricated electrochemical cell-based biosensors for analysis of living cells in vitro.

Wang J, Wu C, Hu N, Zhou J, Du L, Wang P - Biosensors (Basel) (2012)

Bottom Line: When combined with improved biosensor design and advanced measurement systems, the on-line biochemical analysis of living cells in vitro has been applied for biological mechanism study, drug screening and even environmental monitoring.In recent decades, new types of miniaturized electrochemical biosensor are emerging with the development of microfabrication technology.Driven by the need for high throughput and multi-parameter detection proposed by biomedicine, the development trends of electrochemical cell-based biosensors are also introduced, including newly developed integrated biosensors, and the application of nanotechnology and microfluidic technology.

View Article: PubMed Central - PubMed

Affiliation: Biosensor National Special Lab, Key Lab for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zheda Road No. 38, Zhejiang University, Hangzhou 310027, China. wangjun-47@163.com.

ABSTRACT
Cellular biochemical parameters can be used to reveal the physiological and functional information of various cells. Due to demonstrated high accuracy and non-invasiveness, electrochemical detection methods have been used for cell-based investigation. When combined with improved biosensor design and advanced measurement systems, the on-line biochemical analysis of living cells in vitro has been applied for biological mechanism study, drug screening and even environmental monitoring. In recent decades, new types of miniaturized electrochemical biosensor are emerging with the development of microfabrication technology. This review aims to give an overview of the microfabricated electrochemical cell-based biosensors, such as microelectrode arrays (MEA), the electric cell-substrate impedance sensing (ECIS) technique, and the light addressable potentiometric sensor (LAPS). The details in their working principles, measurement systems, and applications in cell monitoring are covered. Driven by the need for high throughput and multi-parameter detection proposed by biomedicine, the development trends of electrochemical cell-based biosensors are also introduced, including newly developed integrated biosensors, and the application of nanotechnology and microfluidic technology.

No MeSH data available.


Related in: MedlinePlus

(a) A metabolic chip integrated with IDES, ISFET sensors and Clark-type electrodes. (Reprinted from [163]. © 2007, with permission from Elsevier). (b) Integrated biochip with MEA, ECIS and LAPS.
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biosensors-02-00127-f024: (a) A metabolic chip integrated with IDES, ISFET sensors and Clark-type electrodes. (Reprinted from [163]. © 2007, with permission from Elsevier). (b) Integrated biochip with MEA, ECIS and LAPS.

Mentions: Brischwein et al. [160] first reported on multi-parametric cell based assays with data obtained solely with integrated sensors on silicon chips. Extracellular acidification rates (with ISFETs), cellular oxygen consumption rates (with amperometric electrode structures) and cell morphological alterations (with impedimetric electrode structures, IDES) were monitored simultaneously for up to several days. Ceriotti et al. [163] employed a multi-parametric chip-based system to measure cell adhesion, metabolism, and response to metal compounds which was previously classified as cytotoxic in immortalized mouse fibroblasts (BALB/3T3 cell line). In a parallel, online, and in a label-free way, the system measured extracellular acidification rates (with ISFETs), the cellular oxygen consumption (with amperometric electrode structures (Clark-type sensors)), and cell adhesion (with IDESs) (Figure 24(a)). A group from the biosensor national special lab in China has established an automatic multi-parametric platform based on an integrated biochip which measures the cell electric activity (with MEA), the cell morphology (with ECIS) and metabolic rates (with LAPS) (Figure 24(b)). Using the integrated multi-parametric sensors, some cell-based assays are conducted, including cytotoxicity and drug assessment, cell development and stem cell differentiation research.


Microfabricated electrochemical cell-based biosensors for analysis of living cells in vitro.

Wang J, Wu C, Hu N, Zhou J, Du L, Wang P - Biosensors (Basel) (2012)

(a) A metabolic chip integrated with IDES, ISFET sensors and Clark-type electrodes. (Reprinted from [163]. © 2007, with permission from Elsevier). (b) Integrated biochip with MEA, ECIS and LAPS.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263572&req=5

biosensors-02-00127-f024: (a) A metabolic chip integrated with IDES, ISFET sensors and Clark-type electrodes. (Reprinted from [163]. © 2007, with permission from Elsevier). (b) Integrated biochip with MEA, ECIS and LAPS.
Mentions: Brischwein et al. [160] first reported on multi-parametric cell based assays with data obtained solely with integrated sensors on silicon chips. Extracellular acidification rates (with ISFETs), cellular oxygen consumption rates (with amperometric electrode structures) and cell morphological alterations (with impedimetric electrode structures, IDES) were monitored simultaneously for up to several days. Ceriotti et al. [163] employed a multi-parametric chip-based system to measure cell adhesion, metabolism, and response to metal compounds which was previously classified as cytotoxic in immortalized mouse fibroblasts (BALB/3T3 cell line). In a parallel, online, and in a label-free way, the system measured extracellular acidification rates (with ISFETs), the cellular oxygen consumption (with amperometric electrode structures (Clark-type sensors)), and cell adhesion (with IDESs) (Figure 24(a)). A group from the biosensor national special lab in China has established an automatic multi-parametric platform based on an integrated biochip which measures the cell electric activity (with MEA), the cell morphology (with ECIS) and metabolic rates (with LAPS) (Figure 24(b)). Using the integrated multi-parametric sensors, some cell-based assays are conducted, including cytotoxicity and drug assessment, cell development and stem cell differentiation research.

Bottom Line: When combined with improved biosensor design and advanced measurement systems, the on-line biochemical analysis of living cells in vitro has been applied for biological mechanism study, drug screening and even environmental monitoring.In recent decades, new types of miniaturized electrochemical biosensor are emerging with the development of microfabrication technology.Driven by the need for high throughput and multi-parameter detection proposed by biomedicine, the development trends of electrochemical cell-based biosensors are also introduced, including newly developed integrated biosensors, and the application of nanotechnology and microfluidic technology.

View Article: PubMed Central - PubMed

Affiliation: Biosensor National Special Lab, Key Lab for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zheda Road No. 38, Zhejiang University, Hangzhou 310027, China. wangjun-47@163.com.

ABSTRACT
Cellular biochemical parameters can be used to reveal the physiological and functional information of various cells. Due to demonstrated high accuracy and non-invasiveness, electrochemical detection methods have been used for cell-based investigation. When combined with improved biosensor design and advanced measurement systems, the on-line biochemical analysis of living cells in vitro has been applied for biological mechanism study, drug screening and even environmental monitoring. In recent decades, new types of miniaturized electrochemical biosensor are emerging with the development of microfabrication technology. This review aims to give an overview of the microfabricated electrochemical cell-based biosensors, such as microelectrode arrays (MEA), the electric cell-substrate impedance sensing (ECIS) technique, and the light addressable potentiometric sensor (LAPS). The details in their working principles, measurement systems, and applications in cell monitoring are covered. Driven by the need for high throughput and multi-parameter detection proposed by biomedicine, the development trends of electrochemical cell-based biosensors are also introduced, including newly developed integrated biosensors, and the application of nanotechnology and microfluidic technology.

No MeSH data available.


Related in: MedlinePlus