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Microfabricated electrochemical cell-based biosensors for analysis of living cells in vitro.

Wang J, Wu C, Hu N, Zhou J, Du L, Wang P - Biosensors (Basel) (2012)

Bottom Line: When combined with improved biosensor design and advanced measurement systems, the on-line biochemical analysis of living cells in vitro has been applied for biological mechanism study, drug screening and even environmental monitoring.In recent decades, new types of miniaturized electrochemical biosensor are emerging with the development of microfabrication technology.Driven by the need for high throughput and multi-parameter detection proposed by biomedicine, the development trends of electrochemical cell-based biosensors are also introduced, including newly developed integrated biosensors, and the application of nanotechnology and microfluidic technology.

View Article: PubMed Central - PubMed

Affiliation: Biosensor National Special Lab, Key Lab for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zheda Road No. 38, Zhejiang University, Hangzhou 310027, China. wangjun-47@163.com.

ABSTRACT
Cellular biochemical parameters can be used to reveal the physiological and functional information of various cells. Due to demonstrated high accuracy and non-invasiveness, electrochemical detection methods have been used for cell-based investigation. When combined with improved biosensor design and advanced measurement systems, the on-line biochemical analysis of living cells in vitro has been applied for biological mechanism study, drug screening and even environmental monitoring. In recent decades, new types of miniaturized electrochemical biosensor are emerging with the development of microfabrication technology. This review aims to give an overview of the microfabricated electrochemical cell-based biosensors, such as microelectrode arrays (MEA), the electric cell-substrate impedance sensing (ECIS) technique, and the light addressable potentiometric sensor (LAPS). The details in their working principles, measurement systems, and applications in cell monitoring are covered. Driven by the need for high throughput and multi-parameter detection proposed by biomedicine, the development trends of electrochemical cell-based biosensors are also introduced, including newly developed integrated biosensors, and the application of nanotechnology and microfluidic technology.

No MeSH data available.


Related in: MedlinePlus

Multianalyte microphysiometer studies based on LAPS. (a) The schematic drawing of the multiparameter-LAPS system to different extracellular ions (H+, K+, and Ca2+); (b) FFT analysis of signals from three sensitive membranes with three light sources; (c) Simultaneous analyzing of H+, K+, Ca2+ by multi-LAPS. (Reprinted from [29]. © 2001, with permission from Elsevier).
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biosensors-02-00127-f023: Multianalyte microphysiometer studies based on LAPS. (a) The schematic drawing of the multiparameter-LAPS system to different extracellular ions (H+, K+, and Ca2+); (b) FFT analysis of signals from three sensitive membranes with three light sources; (c) Simultaneous analyzing of H+, K+, Ca2+ by multi-LAPS. (Reprinted from [29]. © 2001, with permission from Elsevier).

Mentions: The LAPS surface was deposited with different sensitive membranes by the silicon microfabrication technique and the PVC membrane technique. The different sensitive membranes (H+, K+, Ca2+) are illuminated in parallel with light sources at different frequencies (3 kHz for K+, 3.5 kHz for Ca2+, 4 kHz for H+) (Figure 23(a)). The amplitude of each frequency component might be measured online by software FFT analysis (Figure 23(b)). Dilantin, i.e., phenytoin sodium, a sort of anti-epilepsy drug and anti-arrhythmia drug, had significant effects as a tranquilizer, hypnotic and anti-seizure agent. It was proved that dilantin had membrane stabilizing action on neural cells because it reduced the permeability of pericellular membrane ions (Na+, Ca2+), inhibited Na+ and Ca2+ influx and staved K+ efflux. Thus, the refractory period were prolonged, pericellular membrane stabilized and excitability decreased (Figure 23(c)).


Microfabricated electrochemical cell-based biosensors for analysis of living cells in vitro.

Wang J, Wu C, Hu N, Zhou J, Du L, Wang P - Biosensors (Basel) (2012)

Multianalyte microphysiometer studies based on LAPS. (a) The schematic drawing of the multiparameter-LAPS system to different extracellular ions (H+, K+, and Ca2+); (b) FFT analysis of signals from three sensitive membranes with three light sources; (c) Simultaneous analyzing of H+, K+, Ca2+ by multi-LAPS. (Reprinted from [29]. © 2001, with permission from Elsevier).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263572&req=5

biosensors-02-00127-f023: Multianalyte microphysiometer studies based on LAPS. (a) The schematic drawing of the multiparameter-LAPS system to different extracellular ions (H+, K+, and Ca2+); (b) FFT analysis of signals from three sensitive membranes with three light sources; (c) Simultaneous analyzing of H+, K+, Ca2+ by multi-LAPS. (Reprinted from [29]. © 2001, with permission from Elsevier).
Mentions: The LAPS surface was deposited with different sensitive membranes by the silicon microfabrication technique and the PVC membrane technique. The different sensitive membranes (H+, K+, Ca2+) are illuminated in parallel with light sources at different frequencies (3 kHz for K+, 3.5 kHz for Ca2+, 4 kHz for H+) (Figure 23(a)). The amplitude of each frequency component might be measured online by software FFT analysis (Figure 23(b)). Dilantin, i.e., phenytoin sodium, a sort of anti-epilepsy drug and anti-arrhythmia drug, had significant effects as a tranquilizer, hypnotic and anti-seizure agent. It was proved that dilantin had membrane stabilizing action on neural cells because it reduced the permeability of pericellular membrane ions (Na+, Ca2+), inhibited Na+ and Ca2+ influx and staved K+ efflux. Thus, the refractory period were prolonged, pericellular membrane stabilized and excitability decreased (Figure 23(c)).

Bottom Line: When combined with improved biosensor design and advanced measurement systems, the on-line biochemical analysis of living cells in vitro has been applied for biological mechanism study, drug screening and even environmental monitoring.In recent decades, new types of miniaturized electrochemical biosensor are emerging with the development of microfabrication technology.Driven by the need for high throughput and multi-parameter detection proposed by biomedicine, the development trends of electrochemical cell-based biosensors are also introduced, including newly developed integrated biosensors, and the application of nanotechnology and microfluidic technology.

View Article: PubMed Central - PubMed

Affiliation: Biosensor National Special Lab, Key Lab for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zheda Road No. 38, Zhejiang University, Hangzhou 310027, China. wangjun-47@163.com.

ABSTRACT
Cellular biochemical parameters can be used to reveal the physiological and functional information of various cells. Due to demonstrated high accuracy and non-invasiveness, electrochemical detection methods have been used for cell-based investigation. When combined with improved biosensor design and advanced measurement systems, the on-line biochemical analysis of living cells in vitro has been applied for biological mechanism study, drug screening and even environmental monitoring. In recent decades, new types of miniaturized electrochemical biosensor are emerging with the development of microfabrication technology. This review aims to give an overview of the microfabricated electrochemical cell-based biosensors, such as microelectrode arrays (MEA), the electric cell-substrate impedance sensing (ECIS) technique, and the light addressable potentiometric sensor (LAPS). The details in their working principles, measurement systems, and applications in cell monitoring are covered. Driven by the need for high throughput and multi-parameter detection proposed by biomedicine, the development trends of electrochemical cell-based biosensors are also introduced, including newly developed integrated biosensors, and the application of nanotechnology and microfluidic technology.

No MeSH data available.


Related in: MedlinePlus