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Microfabricated electrochemical cell-based biosensors for analysis of living cells in vitro.

Wang J, Wu C, Hu N, Zhou J, Du L, Wang P - Biosensors (Basel) (2012)

Bottom Line: When combined with improved biosensor design and advanced measurement systems, the on-line biochemical analysis of living cells in vitro has been applied for biological mechanism study, drug screening and even environmental monitoring.In recent decades, new types of miniaturized electrochemical biosensor are emerging with the development of microfabrication technology.Driven by the need for high throughput and multi-parameter detection proposed by biomedicine, the development trends of electrochemical cell-based biosensors are also introduced, including newly developed integrated biosensors, and the application of nanotechnology and microfluidic technology.

View Article: PubMed Central - PubMed

Affiliation: Biosensor National Special Lab, Key Lab for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zheda Road No. 38, Zhejiang University, Hangzhou 310027, China. wangjun-47@163.com.

ABSTRACT
Cellular biochemical parameters can be used to reveal the physiological and functional information of various cells. Due to demonstrated high accuracy and non-invasiveness, electrochemical detection methods have been used for cell-based investigation. When combined with improved biosensor design and advanced measurement systems, the on-line biochemical analysis of living cells in vitro has been applied for biological mechanism study, drug screening and even environmental monitoring. In recent decades, new types of miniaturized electrochemical biosensor are emerging with the development of microfabrication technology. This review aims to give an overview of the microfabricated electrochemical cell-based biosensors, such as microelectrode arrays (MEA), the electric cell-substrate impedance sensing (ECIS) technique, and the light addressable potentiometric sensor (LAPS). The details in their working principles, measurement systems, and applications in cell monitoring are covered. Driven by the need for high throughput and multi-parameter detection proposed by biomedicine, the development trends of electrochemical cell-based biosensors are also introduced, including newly developed integrated biosensors, and the application of nanotechnology and microfluidic technology.

No MeSH data available.


Related in: MedlinePlus

Cellular metabolism detection under drug stimulation. (a) Original data shows the change of pH corresponding to the photocurrent; (b) The extracellular acidification rate under the blank (1), glucose (2) and clostridium difficile toxin B (3). (Reprinted from [111]. © 2012, with permission from World Scientific Publishing Co.)
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biosensors-02-00127-f022: Cellular metabolism detection under drug stimulation. (a) Original data shows the change of pH corresponding to the photocurrent; (b) The extracellular acidification rate under the blank (1), glucose (2) and clostridium difficile toxin B (3). (Reprinted from [111]. © 2012, with permission from World Scientific Publishing Co.)

Mentions: In the work of Hu et al. [111], the metabolic activities of MCF-7 cells were monitored by the secretion of the cellular acidic metabolites. The extracellular acidification rate was one of the most significant parameters which indicated cellular metabolic rate. Therefore, the extracellular acidification was monitored to determine the performance of the microphysiometer. Firstly, the extracellular acidification rate was recorded in the presence of cell culture medium alone as basal extracellular acidification rate. Subsequently, glucose and clostridium difficile toxin B was injected respectively into the sensor unit, and the change of extracellular acidification was monitored. Figure 22 displays the cell responses in the stage of blank (1), glucose (2), and clostridium difficile toxin B (3). In the absence of drug, the cells secreted the acidic metabolites in the native state. Meanwhile, the glucose induced a sharp increase in the extracellular acidification rate of MCF-7 cells, with a maximum about 138% after 40 min. Then, clostridium difficile toxin B was injected into the culture medium and induced a 40% decrease of the basal extracellular acidification rate after 70 min.


Microfabricated electrochemical cell-based biosensors for analysis of living cells in vitro.

Wang J, Wu C, Hu N, Zhou J, Du L, Wang P - Biosensors (Basel) (2012)

Cellular metabolism detection under drug stimulation. (a) Original data shows the change of pH corresponding to the photocurrent; (b) The extracellular acidification rate under the blank (1), glucose (2) and clostridium difficile toxin B (3). (Reprinted from [111]. © 2012, with permission from World Scientific Publishing Co.)
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263572&req=5

biosensors-02-00127-f022: Cellular metabolism detection under drug stimulation. (a) Original data shows the change of pH corresponding to the photocurrent; (b) The extracellular acidification rate under the blank (1), glucose (2) and clostridium difficile toxin B (3). (Reprinted from [111]. © 2012, with permission from World Scientific Publishing Co.)
Mentions: In the work of Hu et al. [111], the metabolic activities of MCF-7 cells were monitored by the secretion of the cellular acidic metabolites. The extracellular acidification rate was one of the most significant parameters which indicated cellular metabolic rate. Therefore, the extracellular acidification was monitored to determine the performance of the microphysiometer. Firstly, the extracellular acidification rate was recorded in the presence of cell culture medium alone as basal extracellular acidification rate. Subsequently, glucose and clostridium difficile toxin B was injected respectively into the sensor unit, and the change of extracellular acidification was monitored. Figure 22 displays the cell responses in the stage of blank (1), glucose (2), and clostridium difficile toxin B (3). In the absence of drug, the cells secreted the acidic metabolites in the native state. Meanwhile, the glucose induced a sharp increase in the extracellular acidification rate of MCF-7 cells, with a maximum about 138% after 40 min. Then, clostridium difficile toxin B was injected into the culture medium and induced a 40% decrease of the basal extracellular acidification rate after 70 min.

Bottom Line: When combined with improved biosensor design and advanced measurement systems, the on-line biochemical analysis of living cells in vitro has been applied for biological mechanism study, drug screening and even environmental monitoring.In recent decades, new types of miniaturized electrochemical biosensor are emerging with the development of microfabrication technology.Driven by the need for high throughput and multi-parameter detection proposed by biomedicine, the development trends of electrochemical cell-based biosensors are also introduced, including newly developed integrated biosensors, and the application of nanotechnology and microfluidic technology.

View Article: PubMed Central - PubMed

Affiliation: Biosensor National Special Lab, Key Lab for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zheda Road No. 38, Zhejiang University, Hangzhou 310027, China. wangjun-47@163.com.

ABSTRACT
Cellular biochemical parameters can be used to reveal the physiological and functional information of various cells. Due to demonstrated high accuracy and non-invasiveness, electrochemical detection methods have been used for cell-based investigation. When combined with improved biosensor design and advanced measurement systems, the on-line biochemical analysis of living cells in vitro has been applied for biological mechanism study, drug screening and even environmental monitoring. In recent decades, new types of miniaturized electrochemical biosensor are emerging with the development of microfabrication technology. This review aims to give an overview of the microfabricated electrochemical cell-based biosensors, such as microelectrode arrays (MEA), the electric cell-substrate impedance sensing (ECIS) technique, and the light addressable potentiometric sensor (LAPS). The details in their working principles, measurement systems, and applications in cell monitoring are covered. Driven by the need for high throughput and multi-parameter detection proposed by biomedicine, the development trends of electrochemical cell-based biosensors are also introduced, including newly developed integrated biosensors, and the application of nanotechnology and microfluidic technology.

No MeSH data available.


Related in: MedlinePlus