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Microfabricated electrochemical cell-based biosensors for analysis of living cells in vitro.

Wang J, Wu C, Hu N, Zhou J, Du L, Wang P - Biosensors (Basel) (2012)

Bottom Line: When combined with improved biosensor design and advanced measurement systems, the on-line biochemical analysis of living cells in vitro has been applied for biological mechanism study, drug screening and even environmental monitoring.In recent decades, new types of miniaturized electrochemical biosensor are emerging with the development of microfabrication technology.Driven by the need for high throughput and multi-parameter detection proposed by biomedicine, the development trends of electrochemical cell-based biosensors are also introduced, including newly developed integrated biosensors, and the application of nanotechnology and microfluidic technology.

View Article: PubMed Central - PubMed

Affiliation: Biosensor National Special Lab, Key Lab for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zheda Road No. 38, Zhejiang University, Hangzhou 310027, China. wangjun-47@163.com.

ABSTRACT
Cellular biochemical parameters can be used to reveal the physiological and functional information of various cells. Due to demonstrated high accuracy and non-invasiveness, electrochemical detection methods have been used for cell-based investigation. When combined with improved biosensor design and advanced measurement systems, the on-line biochemical analysis of living cells in vitro has been applied for biological mechanism study, drug screening and even environmental monitoring. In recent decades, new types of miniaturized electrochemical biosensor are emerging with the development of microfabrication technology. This review aims to give an overview of the microfabricated electrochemical cell-based biosensors, such as microelectrode arrays (MEA), the electric cell-substrate impedance sensing (ECIS) technique, and the light addressable potentiometric sensor (LAPS). The details in their working principles, measurement systems, and applications in cell monitoring are covered. Driven by the need for high throughput and multi-parameter detection proposed by biomedicine, the development trends of electrochemical cell-based biosensors are also introduced, including newly developed integrated biosensors, and the application of nanotechnology and microfluidic technology.

No MeSH data available.


Related in: MedlinePlus

ECAR of HEK293 cells under stimulation of BK. (a) ECAR measurements were monitored. BK (white circles) stimulates ECAR, whereas the BK B1 receptor agonist des-Agr9-BK (dark circles) does not. Cells were exposed to perfusate-containing drug during the time span encompassed by the gray box; (b) ECAR stimulated by 10−6 M of BK in various buffers, including Ham’s F12 medium, without and with 10−5 M MIA, a balanced salt solution containing NaCl or TMA substituted mM per mM for sodium. * P < 0.05 vs. BK alone; zP < 0.01 vs. BK in balanced salt solution with NaCl; (c) Effects of BK B2 (HOE-140) and BK B1 (des-Arg10-HOE-140) receptor antagonists on BK-stimulated ECAR. Antagonists (10−5 M) were added 30 min prior to addition of BK. All experiments were performed at least 4 times. zP < 0.01 vs. BK alone. Error bars in (b) and (c) represent the S.E.M. (Reprinted from [115]. © 2009, with permission from Elsevier).
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biosensors-02-00127-f019: ECAR of HEK293 cells under stimulation of BK. (a) ECAR measurements were monitored. BK (white circles) stimulates ECAR, whereas the BK B1 receptor agonist des-Agr9-BK (dark circles) does not. Cells were exposed to perfusate-containing drug during the time span encompassed by the gray box; (b) ECAR stimulated by 10−6 M of BK in various buffers, including Ham’s F12 medium, without and with 10−5 M MIA, a balanced salt solution containing NaCl or TMA substituted mM per mM for sodium. * P < 0.05 vs. BK alone; zP < 0.01 vs. BK in balanced salt solution with NaCl; (c) Effects of BK B2 (HOE-140) and BK B1 (des-Arg10-HOE-140) receptor antagonists on BK-stimulated ECAR. Antagonists (10−5 M) were added 30 min prior to addition of BK. All experiments were performed at least 4 times. zP < 0.01 vs. BK alone. Error bars in (b) and (c) represent the S.E.M. (Reprinted from [115]. © 2009, with permission from Elsevier).

Mentions: ECAR of CHO cells expressing orphan GPCRs was measured [116]. When treated with synthetic compounds, such as bioactive peptides, it was found that CHO cells expressing an orphan GPCR, FM-3 were responsible to neuromedin U in the microphysiometric assay. It was also discovered that bradykinin (BK) activated extracellular signal-regulated protein kinase 1 and 2 (ERK) in the human embryonic kidney (HEK) 293 cells [115]. ECAR was a reflection of the Na+/H+ exchange (NHE). When combined with intracellular Ca2+ concentration from fluorescent measurements, and ERK activation assessment through western blotting with a phosphor-specific ERK antibody, it was found that signals obtained by exposure of HEK 293 cells to BK, were blocked by HOE-140 (B2 receptor antagonist) but not by des-Arg10-HOE-140 (B1 receptor antagonist) (Figure 19). Thus it was concluded that HEK 293 cells express endogenous functional BK B2 receptors.


Microfabricated electrochemical cell-based biosensors for analysis of living cells in vitro.

Wang J, Wu C, Hu N, Zhou J, Du L, Wang P - Biosensors (Basel) (2012)

ECAR of HEK293 cells under stimulation of BK. (a) ECAR measurements were monitored. BK (white circles) stimulates ECAR, whereas the BK B1 receptor agonist des-Agr9-BK (dark circles) does not. Cells were exposed to perfusate-containing drug during the time span encompassed by the gray box; (b) ECAR stimulated by 10−6 M of BK in various buffers, including Ham’s F12 medium, without and with 10−5 M MIA, a balanced salt solution containing NaCl or TMA substituted mM per mM for sodium. * P < 0.05 vs. BK alone; zP < 0.01 vs. BK in balanced salt solution with NaCl; (c) Effects of BK B2 (HOE-140) and BK B1 (des-Arg10-HOE-140) receptor antagonists on BK-stimulated ECAR. Antagonists (10−5 M) were added 30 min prior to addition of BK. All experiments were performed at least 4 times. zP < 0.01 vs. BK alone. Error bars in (b) and (c) represent the S.E.M. (Reprinted from [115]. © 2009, with permission from Elsevier).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263572&req=5

biosensors-02-00127-f019: ECAR of HEK293 cells under stimulation of BK. (a) ECAR measurements were monitored. BK (white circles) stimulates ECAR, whereas the BK B1 receptor agonist des-Agr9-BK (dark circles) does not. Cells were exposed to perfusate-containing drug during the time span encompassed by the gray box; (b) ECAR stimulated by 10−6 M of BK in various buffers, including Ham’s F12 medium, without and with 10−5 M MIA, a balanced salt solution containing NaCl or TMA substituted mM per mM for sodium. * P < 0.05 vs. BK alone; zP < 0.01 vs. BK in balanced salt solution with NaCl; (c) Effects of BK B2 (HOE-140) and BK B1 (des-Arg10-HOE-140) receptor antagonists on BK-stimulated ECAR. Antagonists (10−5 M) were added 30 min prior to addition of BK. All experiments were performed at least 4 times. zP < 0.01 vs. BK alone. Error bars in (b) and (c) represent the S.E.M. (Reprinted from [115]. © 2009, with permission from Elsevier).
Mentions: ECAR of CHO cells expressing orphan GPCRs was measured [116]. When treated with synthetic compounds, such as bioactive peptides, it was found that CHO cells expressing an orphan GPCR, FM-3 were responsible to neuromedin U in the microphysiometric assay. It was also discovered that bradykinin (BK) activated extracellular signal-regulated protein kinase 1 and 2 (ERK) in the human embryonic kidney (HEK) 293 cells [115]. ECAR was a reflection of the Na+/H+ exchange (NHE). When combined with intracellular Ca2+ concentration from fluorescent measurements, and ERK activation assessment through western blotting with a phosphor-specific ERK antibody, it was found that signals obtained by exposure of HEK 293 cells to BK, were blocked by HOE-140 (B2 receptor antagonist) but not by des-Arg10-HOE-140 (B1 receptor antagonist) (Figure 19). Thus it was concluded that HEK 293 cells express endogenous functional BK B2 receptors.

Bottom Line: When combined with improved biosensor design and advanced measurement systems, the on-line biochemical analysis of living cells in vitro has been applied for biological mechanism study, drug screening and even environmental monitoring.In recent decades, new types of miniaturized electrochemical biosensor are emerging with the development of microfabrication technology.Driven by the need for high throughput and multi-parameter detection proposed by biomedicine, the development trends of electrochemical cell-based biosensors are also introduced, including newly developed integrated biosensors, and the application of nanotechnology and microfluidic technology.

View Article: PubMed Central - PubMed

Affiliation: Biosensor National Special Lab, Key Lab for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zheda Road No. 38, Zhejiang University, Hangzhou 310027, China. wangjun-47@163.com.

ABSTRACT
Cellular biochemical parameters can be used to reveal the physiological and functional information of various cells. Due to demonstrated high accuracy and non-invasiveness, electrochemical detection methods have been used for cell-based investigation. When combined with improved biosensor design and advanced measurement systems, the on-line biochemical analysis of living cells in vitro has been applied for biological mechanism study, drug screening and even environmental monitoring. In recent decades, new types of miniaturized electrochemical biosensor are emerging with the development of microfabrication technology. This review aims to give an overview of the microfabricated electrochemical cell-based biosensors, such as microelectrode arrays (MEA), the electric cell-substrate impedance sensing (ECIS) technique, and the light addressable potentiometric sensor (LAPS). The details in their working principles, measurement systems, and applications in cell monitoring are covered. Driven by the need for high throughput and multi-parameter detection proposed by biomedicine, the development trends of electrochemical cell-based biosensors are also introduced, including newly developed integrated biosensors, and the application of nanotechnology and microfluidic technology.

No MeSH data available.


Related in: MedlinePlus