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Microfabricated electrochemical cell-based biosensors for analysis of living cells in vitro.

Wang J, Wu C, Hu N, Zhou J, Du L, Wang P - Biosensors (Basel) (2012)

Bottom Line: When combined with improved biosensor design and advanced measurement systems, the on-line biochemical analysis of living cells in vitro has been applied for biological mechanism study, drug screening and even environmental monitoring.In recent decades, new types of miniaturized electrochemical biosensor are emerging with the development of microfabrication technology.Driven by the need for high throughput and multi-parameter detection proposed by biomedicine, the development trends of electrochemical cell-based biosensors are also introduced, including newly developed integrated biosensors, and the application of nanotechnology and microfluidic technology.

View Article: PubMed Central - PubMed

Affiliation: Biosensor National Special Lab, Key Lab for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zheda Road No. 38, Zhejiang University, Hangzhou 310027, China. wangjun-47@163.com.

ABSTRACT
Cellular biochemical parameters can be used to reveal the physiological and functional information of various cells. Due to demonstrated high accuracy and non-invasiveness, electrochemical detection methods have been used for cell-based investigation. When combined with improved biosensor design and advanced measurement systems, the on-line biochemical analysis of living cells in vitro has been applied for biological mechanism study, drug screening and even environmental monitoring. In recent decades, new types of miniaturized electrochemical biosensor are emerging with the development of microfabrication technology. This review aims to give an overview of the microfabricated electrochemical cell-based biosensors, such as microelectrode arrays (MEA), the electric cell-substrate impedance sensing (ECIS) technique, and the light addressable potentiometric sensor (LAPS). The details in their working principles, measurement systems, and applications in cell monitoring are covered. Driven by the need for high throughput and multi-parameter detection proposed by biomedicine, the development trends of electrochemical cell-based biosensors are also introduced, including newly developed integrated biosensors, and the application of nanotechnology and microfluidic technology.

No MeSH data available.


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Electrophysiological signal changes of olfactory cells after stimulation of acetic acid and butanedione. (Reprinted from [75]. © 2010, with permission from Elsevier).
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biosensors-02-00127-f014: Electrophysiological signal changes of olfactory cells after stimulation of acetic acid and butanedione. (Reprinted from [75]. © 2010, with permission from Elsevier).

Mentions: MEA is also a stable platform for detecting electrophysiological activities of olfactory and gustatory system. The biological olfactory system has high sensitivity and specificity to discriminate different odors. In the work of Liu et al. [75], the stripped olfactory epithelium was fixed on the surface of a 36-channel MEA. Then, the extracellular potentials of the olfactory receptor neurons in the epithelium were recorded after stimulation of odorants such as acetic acid and butanedione. The olfactory cells generated firing with different modes (Figure 14). Through time-domain and frequency-domain analysis, the firing characteristics were found to be different from that of spontaneous potentials. The detection principle of gustatory cell-based MEA was similar. When exposed to substances with different tastes, such as sodium chloride, quinine and sodium glutamate, the recorded potentials represented different firing modes according to the power spectrum analysis. The multi-channel signal analysis has potentials in revealing some spatial and temporal information of early olfactory/gustatory sensing for bioelectronic nose/tongue.


Microfabricated electrochemical cell-based biosensors for analysis of living cells in vitro.

Wang J, Wu C, Hu N, Zhou J, Du L, Wang P - Biosensors (Basel) (2012)

Electrophysiological signal changes of olfactory cells after stimulation of acetic acid and butanedione. (Reprinted from [75]. © 2010, with permission from Elsevier).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263572&req=5

biosensors-02-00127-f014: Electrophysiological signal changes of olfactory cells after stimulation of acetic acid and butanedione. (Reprinted from [75]. © 2010, with permission from Elsevier).
Mentions: MEA is also a stable platform for detecting electrophysiological activities of olfactory and gustatory system. The biological olfactory system has high sensitivity and specificity to discriminate different odors. In the work of Liu et al. [75], the stripped olfactory epithelium was fixed on the surface of a 36-channel MEA. Then, the extracellular potentials of the olfactory receptor neurons in the epithelium were recorded after stimulation of odorants such as acetic acid and butanedione. The olfactory cells generated firing with different modes (Figure 14). Through time-domain and frequency-domain analysis, the firing characteristics were found to be different from that of spontaneous potentials. The detection principle of gustatory cell-based MEA was similar. When exposed to substances with different tastes, such as sodium chloride, quinine and sodium glutamate, the recorded potentials represented different firing modes according to the power spectrum analysis. The multi-channel signal analysis has potentials in revealing some spatial and temporal information of early olfactory/gustatory sensing for bioelectronic nose/tongue.

Bottom Line: When combined with improved biosensor design and advanced measurement systems, the on-line biochemical analysis of living cells in vitro has been applied for biological mechanism study, drug screening and even environmental monitoring.In recent decades, new types of miniaturized electrochemical biosensor are emerging with the development of microfabrication technology.Driven by the need for high throughput and multi-parameter detection proposed by biomedicine, the development trends of electrochemical cell-based biosensors are also introduced, including newly developed integrated biosensors, and the application of nanotechnology and microfluidic technology.

View Article: PubMed Central - PubMed

Affiliation: Biosensor National Special Lab, Key Lab for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zheda Road No. 38, Zhejiang University, Hangzhou 310027, China. wangjun-47@163.com.

ABSTRACT
Cellular biochemical parameters can be used to reveal the physiological and functional information of various cells. Due to demonstrated high accuracy and non-invasiveness, electrochemical detection methods have been used for cell-based investigation. When combined with improved biosensor design and advanced measurement systems, the on-line biochemical analysis of living cells in vitro has been applied for biological mechanism study, drug screening and even environmental monitoring. In recent decades, new types of miniaturized electrochemical biosensor are emerging with the development of microfabrication technology. This review aims to give an overview of the microfabricated electrochemical cell-based biosensors, such as microelectrode arrays (MEA), the electric cell-substrate impedance sensing (ECIS) technique, and the light addressable potentiometric sensor (LAPS). The details in their working principles, measurement systems, and applications in cell monitoring are covered. Driven by the need for high throughput and multi-parameter detection proposed by biomedicine, the development trends of electrochemical cell-based biosensors are also introduced, including newly developed integrated biosensors, and the application of nanotechnology and microfluidic technology.

No MeSH data available.


Related in: MedlinePlus