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Microfabricated electrochemical cell-based biosensors for analysis of living cells in vitro.

Wang J, Wu C, Hu N, Zhou J, Du L, Wang P - Biosensors (Basel) (2012)

Bottom Line: When combined with improved biosensor design and advanced measurement systems, the on-line biochemical analysis of living cells in vitro has been applied for biological mechanism study, drug screening and even environmental monitoring.In recent decades, new types of miniaturized electrochemical biosensor are emerging with the development of microfabrication technology.Driven by the need for high throughput and multi-parameter detection proposed by biomedicine, the development trends of electrochemical cell-based biosensors are also introduced, including newly developed integrated biosensors, and the application of nanotechnology and microfluidic technology.

View Article: PubMed Central - PubMed

Affiliation: Biosensor National Special Lab, Key Lab for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zheda Road No. 38, Zhejiang University, Hangzhou 310027, China. wangjun-47@163.com.

ABSTRACT
Cellular biochemical parameters can be used to reveal the physiological and functional information of various cells. Due to demonstrated high accuracy and non-invasiveness, electrochemical detection methods have been used for cell-based investigation. When combined with improved biosensor design and advanced measurement systems, the on-line biochemical analysis of living cells in vitro has been applied for biological mechanism study, drug screening and even environmental monitoring. In recent decades, new types of miniaturized electrochemical biosensor are emerging with the development of microfabrication technology. This review aims to give an overview of the microfabricated electrochemical cell-based biosensors, such as microelectrode arrays (MEA), the electric cell-substrate impedance sensing (ECIS) technique, and the light addressable potentiometric sensor (LAPS). The details in their working principles, measurement systems, and applications in cell monitoring are covered. Driven by the need for high throughput and multi-parameter detection proposed by biomedicine, the development trends of electrochemical cell-based biosensors are also introduced, including newly developed integrated biosensors, and the application of nanotechnology and microfluidic technology.

No MeSH data available.


Related in: MedlinePlus

Conformal probes. (a) cMEA#1 is a 3 × 20 rectangular array well suited for electrophysiological investigations of the pyramidal and granular cells of hippocampus; (b) Using cMEA#2, monosynaptic input/output (IO) curves were recorded in CA1 in response to SchC stimulation; (c) Monosynaptic and disynaptic responses were recorded from DG and CA3 respectively by stimulating (perforant path) PP with external electrodes; (d) The cMEA#4 was designed to record the monosynaptic response of the DG, disynaptic response in the CA3 area and trisynaptic response in the CA1 when the PP is stimulated. (Reprinted from [55]. © 2006, with permission from Elsevier).
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biosensors-02-00127-f011: Conformal probes. (a) cMEA#1 is a 3 × 20 rectangular array well suited for electrophysiological investigations of the pyramidal and granular cells of hippocampus; (b) Using cMEA#2, monosynaptic input/output (IO) curves were recorded in CA1 in response to SchC stimulation; (c) Monosynaptic and disynaptic responses were recorded from DG and CA3 respectively by stimulating (perforant path) PP with external electrodes; (d) The cMEA#4 was designed to record the monosynaptic response of the DG, disynaptic response in the CA3 area and trisynaptic response in the CA1 when the PP is stimulated. (Reprinted from [55]. © 2006, with permission from Elsevier).

Mentions: Compared with the dissociated cultures, slices hold the closer characteristics to that of the intact tissues. Hippocampal slice is the optimal object to study excitatory and inhibitory transmission as well as synaptic plasticity. Microelectrodes are designed in a tissue-conformal and partially high-density distribution for specific stimulation and recording experiments in acute hippocampal slices [55]. Four custom-designed planar MEAs (cMEAs) in different configurations which conform in design to the slice cytoarchitecture are well suited for specific electrophysiological applications (Figure 11). The high-density provides high spatial resolution for selective stimulation of afferent pathways (Schaffer collaterals; medial versus lateral perforant path) and recording of the corresponding responses. It also enables current source density (CSD) analysis of laminar profiles obtained through sequential stimulation along the CA1 pyramidal tree.


Microfabricated electrochemical cell-based biosensors for analysis of living cells in vitro.

Wang J, Wu C, Hu N, Zhou J, Du L, Wang P - Biosensors (Basel) (2012)

Conformal probes. (a) cMEA#1 is a 3 × 20 rectangular array well suited for electrophysiological investigations of the pyramidal and granular cells of hippocampus; (b) Using cMEA#2, monosynaptic input/output (IO) curves were recorded in CA1 in response to SchC stimulation; (c) Monosynaptic and disynaptic responses were recorded from DG and CA3 respectively by stimulating (perforant path) PP with external electrodes; (d) The cMEA#4 was designed to record the monosynaptic response of the DG, disynaptic response in the CA3 area and trisynaptic response in the CA1 when the PP is stimulated. (Reprinted from [55]. © 2006, with permission from Elsevier).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263572&req=5

biosensors-02-00127-f011: Conformal probes. (a) cMEA#1 is a 3 × 20 rectangular array well suited for electrophysiological investigations of the pyramidal and granular cells of hippocampus; (b) Using cMEA#2, monosynaptic input/output (IO) curves were recorded in CA1 in response to SchC stimulation; (c) Monosynaptic and disynaptic responses were recorded from DG and CA3 respectively by stimulating (perforant path) PP with external electrodes; (d) The cMEA#4 was designed to record the monosynaptic response of the DG, disynaptic response in the CA3 area and trisynaptic response in the CA1 when the PP is stimulated. (Reprinted from [55]. © 2006, with permission from Elsevier).
Mentions: Compared with the dissociated cultures, slices hold the closer characteristics to that of the intact tissues. Hippocampal slice is the optimal object to study excitatory and inhibitory transmission as well as synaptic plasticity. Microelectrodes are designed in a tissue-conformal and partially high-density distribution for specific stimulation and recording experiments in acute hippocampal slices [55]. Four custom-designed planar MEAs (cMEAs) in different configurations which conform in design to the slice cytoarchitecture are well suited for specific electrophysiological applications (Figure 11). The high-density provides high spatial resolution for selective stimulation of afferent pathways (Schaffer collaterals; medial versus lateral perforant path) and recording of the corresponding responses. It also enables current source density (CSD) analysis of laminar profiles obtained through sequential stimulation along the CA1 pyramidal tree.

Bottom Line: When combined with improved biosensor design and advanced measurement systems, the on-line biochemical analysis of living cells in vitro has been applied for biological mechanism study, drug screening and even environmental monitoring.In recent decades, new types of miniaturized electrochemical biosensor are emerging with the development of microfabrication technology.Driven by the need for high throughput and multi-parameter detection proposed by biomedicine, the development trends of electrochemical cell-based biosensors are also introduced, including newly developed integrated biosensors, and the application of nanotechnology and microfluidic technology.

View Article: PubMed Central - PubMed

Affiliation: Biosensor National Special Lab, Key Lab for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zheda Road No. 38, Zhejiang University, Hangzhou 310027, China. wangjun-47@163.com.

ABSTRACT
Cellular biochemical parameters can be used to reveal the physiological and functional information of various cells. Due to demonstrated high accuracy and non-invasiveness, electrochemical detection methods have been used for cell-based investigation. When combined with improved biosensor design and advanced measurement systems, the on-line biochemical analysis of living cells in vitro has been applied for biological mechanism study, drug screening and even environmental monitoring. In recent decades, new types of miniaturized electrochemical biosensor are emerging with the development of microfabrication technology. This review aims to give an overview of the microfabricated electrochemical cell-based biosensors, such as microelectrode arrays (MEA), the electric cell-substrate impedance sensing (ECIS) technique, and the light addressable potentiometric sensor (LAPS). The details in their working principles, measurement systems, and applications in cell monitoring are covered. Driven by the need for high throughput and multi-parameter detection proposed by biomedicine, the development trends of electrochemical cell-based biosensors are also introduced, including newly developed integrated biosensors, and the application of nanotechnology and microfluidic technology.

No MeSH data available.


Related in: MedlinePlus