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Microfabricated electrochemical cell-based biosensors for analysis of living cells in vitro.

Wang J, Wu C, Hu N, Zhou J, Du L, Wang P - Biosensors (Basel) (2012)

Bottom Line: When combined with improved biosensor design and advanced measurement systems, the on-line biochemical analysis of living cells in vitro has been applied for biological mechanism study, drug screening and even environmental monitoring.In recent decades, new types of miniaturized electrochemical biosensor are emerging with the development of microfabrication technology.Driven by the need for high throughput and multi-parameter detection proposed by biomedicine, the development trends of electrochemical cell-based biosensors are also introduced, including newly developed integrated biosensors, and the application of nanotechnology and microfluidic technology.

View Article: PubMed Central - PubMed

Affiliation: Biosensor National Special Lab, Key Lab for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zheda Road No. 38, Zhejiang University, Hangzhou 310027, China. wangjun-47@163.com.

ABSTRACT
Cellular biochemical parameters can be used to reveal the physiological and functional information of various cells. Due to demonstrated high accuracy and non-invasiveness, electrochemical detection methods have been used for cell-based investigation. When combined with improved biosensor design and advanced measurement systems, the on-line biochemical analysis of living cells in vitro has been applied for biological mechanism study, drug screening and even environmental monitoring. In recent decades, new types of miniaturized electrochemical biosensor are emerging with the development of microfabrication technology. This review aims to give an overview of the microfabricated electrochemical cell-based biosensors, such as microelectrode arrays (MEA), the electric cell-substrate impedance sensing (ECIS) technique, and the light addressable potentiometric sensor (LAPS). The details in their working principles, measurement systems, and applications in cell monitoring are covered. Driven by the need for high throughput and multi-parameter detection proposed by biomedicine, the development trends of electrochemical cell-based biosensors are also introduced, including newly developed integrated biosensors, and the application of nanotechnology and microfluidic technology.

No MeSH data available.


Related in: MedlinePlus

(a) The equivalent circuit of metal-electrolyte interface; (b) The equivalent circuit of the signal pathway in MEA system. Vin: the intracellular potential; CM: the capacity of the cellular membrane; IM: the current source of the cellular membrane; A: the junction between the cell and electrode; Rseal: the sealing resistance between the cell and chip; Rinput: the equivalent of the input of preamplifier; Vout: the output potential of the microelectrode; Vreference: the grounded bulk media potential.
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biosensors-02-00127-f001: (a) The equivalent circuit of metal-electrolyte interface; (b) The equivalent circuit of the signal pathway in MEA system. Vin: the intracellular potential; CM: the capacity of the cellular membrane; IM: the current source of the cellular membrane; A: the junction between the cell and electrode; Rseal: the sealing resistance between the cell and chip; Rinput: the equivalent of the input of preamplifier; Vout: the output potential of the microelectrode; Vreference: the grounded bulk media potential.

Mentions: The equivalent circuit of metal-electrolyte interface can be explained with the Randles model, as shown in Figure 1(a). In the circuit, an interfacial capacitance (CI) is in parallel with charge transfer resistance (Rt) and diffusion related Warburg element (RW and CW). The spreading resistance (RS) represents the effect of current spreading from the localized electrode to a distant counter electrode.


Microfabricated electrochemical cell-based biosensors for analysis of living cells in vitro.

Wang J, Wu C, Hu N, Zhou J, Du L, Wang P - Biosensors (Basel) (2012)

(a) The equivalent circuit of metal-electrolyte interface; (b) The equivalent circuit of the signal pathway in MEA system. Vin: the intracellular potential; CM: the capacity of the cellular membrane; IM: the current source of the cellular membrane; A: the junction between the cell and electrode; Rseal: the sealing resistance between the cell and chip; Rinput: the equivalent of the input of preamplifier; Vout: the output potential of the microelectrode; Vreference: the grounded bulk media potential.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263572&req=5

biosensors-02-00127-f001: (a) The equivalent circuit of metal-electrolyte interface; (b) The equivalent circuit of the signal pathway in MEA system. Vin: the intracellular potential; CM: the capacity of the cellular membrane; IM: the current source of the cellular membrane; A: the junction between the cell and electrode; Rseal: the sealing resistance between the cell and chip; Rinput: the equivalent of the input of preamplifier; Vout: the output potential of the microelectrode; Vreference: the grounded bulk media potential.
Mentions: The equivalent circuit of metal-electrolyte interface can be explained with the Randles model, as shown in Figure 1(a). In the circuit, an interfacial capacitance (CI) is in parallel with charge transfer resistance (Rt) and diffusion related Warburg element (RW and CW). The spreading resistance (RS) represents the effect of current spreading from the localized electrode to a distant counter electrode.

Bottom Line: When combined with improved biosensor design and advanced measurement systems, the on-line biochemical analysis of living cells in vitro has been applied for biological mechanism study, drug screening and even environmental monitoring.In recent decades, new types of miniaturized electrochemical biosensor are emerging with the development of microfabrication technology.Driven by the need for high throughput and multi-parameter detection proposed by biomedicine, the development trends of electrochemical cell-based biosensors are also introduced, including newly developed integrated biosensors, and the application of nanotechnology and microfluidic technology.

View Article: PubMed Central - PubMed

Affiliation: Biosensor National Special Lab, Key Lab for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zheda Road No. 38, Zhejiang University, Hangzhou 310027, China. wangjun-47@163.com.

ABSTRACT
Cellular biochemical parameters can be used to reveal the physiological and functional information of various cells. Due to demonstrated high accuracy and non-invasiveness, electrochemical detection methods have been used for cell-based investigation. When combined with improved biosensor design and advanced measurement systems, the on-line biochemical analysis of living cells in vitro has been applied for biological mechanism study, drug screening and even environmental monitoring. In recent decades, new types of miniaturized electrochemical biosensor are emerging with the development of microfabrication technology. This review aims to give an overview of the microfabricated electrochemical cell-based biosensors, such as microelectrode arrays (MEA), the electric cell-substrate impedance sensing (ECIS) technique, and the light addressable potentiometric sensor (LAPS). The details in their working principles, measurement systems, and applications in cell monitoring are covered. Driven by the need for high throughput and multi-parameter detection proposed by biomedicine, the development trends of electrochemical cell-based biosensors are also introduced, including newly developed integrated biosensors, and the application of nanotechnology and microfluidic technology.

No MeSH data available.


Related in: MedlinePlus