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Detection of cardiac biomarkers using single polyaniline nanowire-based conductometric biosensors.

Lee I, Luo X, Huang J, Cui XT, Yun M - Biosensors (Basel) (2012)

Bottom Line: The single PANI nanowire-based biosensors displayed linear sensing profiles for concentrations ranging from hundreds (fg/mL) to tens (ng/mL).This single PANI nanowire-based biosensor demonstrated superior biosensing reliability with the feasibility of label free detection and improved processing cost efficiency due to good biocompatibility of PANI to monoclonal antibodies (mAbs).Therefore, this development of single PANI nanowire-based biosensors can be applied to other biosensors for cancer or other diseases.

View Article: PubMed Central - PubMed

Affiliation: Department of Electrical and Computer Engineering, University of Pittsburgh, Pittsburgh, PA 15261, USA. inl8@pitt.edu.

ABSTRACT
The detection of myoglobin (Myo), cardiac troponin I (cTnI), creatine kinase-MB (CK-MB), and b-type natriuretic peptide (BNP) plays a vital role in diagnosing cardiovascular diseases. Here we present single site-specific polyaniline (PANI) nanowire biosensors that can detect cardiac biomarkers such as Myo, cTnI, CK-MB, and BNP with ultra-high sensitivity and good specificity. Using single PANI nanowire-based biosensors integrated with microfluidic channels, very low concentrations of Myo (100 pg/mL), cTnI (250 fg/mL), CK-MB (150 fg/mL), and BNP (50 fg/mL) were detected. The single PANI nanowire-based biosensors displayed linear sensing profiles for concentrations ranging from hundreds (fg/mL) to tens (ng/mL). In addition, devices showed a fast (few minutes) response satisfying respective reference conditions for Myo, cTnI, CK-MB, and BNP diagnosis of heart failure and for determining the stage of the disease. This single PANI nanowire-based biosensor demonstrated superior biosensing reliability with the feasibility of label free detection and improved processing cost efficiency due to good biocompatibility of PANI to monoclonal antibodies (mAbs). Therefore, this development of single PANI nanowire-based biosensors can be applied to other biosensors for cancer or other diseases.

No MeSH data available.


Related in: MedlinePlus

Single PANI nanowire biosensor chips integrated with microfluidic channel present the lowest detection of cardiac biomarkers. (a) Detection of Myo (a: PBS, b: 100 ng/mL BSA, and c: 100 pg/mL Myo); (b) Detection of cTnI (a: PBS, b: 10 ng/mL BSA, c: 5 fg/mL cTnI, d: 250 fg/mL cTnI, e: 20 pg/mL cTnI); (c) Detection of CK-MB (a: PBS, b: 10 ng/mL BSA, c: 150 fg/mL CK-MB); (d) Detection of BNP (a: PBS, b: 100 ng/mL BSA, c: 50 fg/mL BNP, and d: 1 pg/mL BNP).
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biosensors-02-00205-f003: Single PANI nanowire biosensor chips integrated with microfluidic channel present the lowest detection of cardiac biomarkers. (a) Detection of Myo (a: PBS, b: 100 ng/mL BSA, and c: 100 pg/mL Myo); (b) Detection of cTnI (a: PBS, b: 10 ng/mL BSA, c: 5 fg/mL cTnI, d: 250 fg/mL cTnI, e: 20 pg/mL cTnI); (c) Detection of CK-MB (a: PBS, b: 10 ng/mL BSA, c: 150 fg/mL CK-MB); (d) Detection of BNP (a: PBS, b: 100 ng/mL BSA, c: 50 fg/mL BNP, and d: 1 pg/mL BNP).

Mentions: The detection of Myo, cTnI, CK-MB, and BNP on the single PANI nanowire biosensors was carried out by monitoring the change of conductance in the nanowires as shown in Figure 3. The integration of the microfluidic channel assists accurate and reliable biosensing by directing the flow of the solution only onto the active area of the single PANI nanowire and minimizing the damage of the nanowire with a slow flow rate. In addition, the lowest detections of Myo, cTnI, CK-MB, and BNP could be obtained at 100 pg/mL, 250 fg/mL, 150 fg/mL, and 50 fg/mL as demonstrated in Figure 3(a–d), respectively.


Detection of cardiac biomarkers using single polyaniline nanowire-based conductometric biosensors.

Lee I, Luo X, Huang J, Cui XT, Yun M - Biosensors (Basel) (2012)

Single PANI nanowire biosensor chips integrated with microfluidic channel present the lowest detection of cardiac biomarkers. (a) Detection of Myo (a: PBS, b: 100 ng/mL BSA, and c: 100 pg/mL Myo); (b) Detection of cTnI (a: PBS, b: 10 ng/mL BSA, c: 5 fg/mL cTnI, d: 250 fg/mL cTnI, e: 20 pg/mL cTnI); (c) Detection of CK-MB (a: PBS, b: 10 ng/mL BSA, c: 150 fg/mL CK-MB); (d) Detection of BNP (a: PBS, b: 100 ng/mL BSA, c: 50 fg/mL BNP, and d: 1 pg/mL BNP).
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4263570&req=5

biosensors-02-00205-f003: Single PANI nanowire biosensor chips integrated with microfluidic channel present the lowest detection of cardiac biomarkers. (a) Detection of Myo (a: PBS, b: 100 ng/mL BSA, and c: 100 pg/mL Myo); (b) Detection of cTnI (a: PBS, b: 10 ng/mL BSA, c: 5 fg/mL cTnI, d: 250 fg/mL cTnI, e: 20 pg/mL cTnI); (c) Detection of CK-MB (a: PBS, b: 10 ng/mL BSA, c: 150 fg/mL CK-MB); (d) Detection of BNP (a: PBS, b: 100 ng/mL BSA, c: 50 fg/mL BNP, and d: 1 pg/mL BNP).
Mentions: The detection of Myo, cTnI, CK-MB, and BNP on the single PANI nanowire biosensors was carried out by monitoring the change of conductance in the nanowires as shown in Figure 3. The integration of the microfluidic channel assists accurate and reliable biosensing by directing the flow of the solution only onto the active area of the single PANI nanowire and minimizing the damage of the nanowire with a slow flow rate. In addition, the lowest detections of Myo, cTnI, CK-MB, and BNP could be obtained at 100 pg/mL, 250 fg/mL, 150 fg/mL, and 50 fg/mL as demonstrated in Figure 3(a–d), respectively.

Bottom Line: The single PANI nanowire-based biosensors displayed linear sensing profiles for concentrations ranging from hundreds (fg/mL) to tens (ng/mL).This single PANI nanowire-based biosensor demonstrated superior biosensing reliability with the feasibility of label free detection and improved processing cost efficiency due to good biocompatibility of PANI to monoclonal antibodies (mAbs).Therefore, this development of single PANI nanowire-based biosensors can be applied to other biosensors for cancer or other diseases.

View Article: PubMed Central - PubMed

Affiliation: Department of Electrical and Computer Engineering, University of Pittsburgh, Pittsburgh, PA 15261, USA. inl8@pitt.edu.

ABSTRACT
The detection of myoglobin (Myo), cardiac troponin I (cTnI), creatine kinase-MB (CK-MB), and b-type natriuretic peptide (BNP) plays a vital role in diagnosing cardiovascular diseases. Here we present single site-specific polyaniline (PANI) nanowire biosensors that can detect cardiac biomarkers such as Myo, cTnI, CK-MB, and BNP with ultra-high sensitivity and good specificity. Using single PANI nanowire-based biosensors integrated with microfluidic channels, very low concentrations of Myo (100 pg/mL), cTnI (250 fg/mL), CK-MB (150 fg/mL), and BNP (50 fg/mL) were detected. The single PANI nanowire-based biosensors displayed linear sensing profiles for concentrations ranging from hundreds (fg/mL) to tens (ng/mL). In addition, devices showed a fast (few minutes) response satisfying respective reference conditions for Myo, cTnI, CK-MB, and BNP diagnosis of heart failure and for determining the stage of the disease. This single PANI nanowire-based biosensor demonstrated superior biosensing reliability with the feasibility of label free detection and improved processing cost efficiency due to good biocompatibility of PANI to monoclonal antibodies (mAbs). Therefore, this development of single PANI nanowire-based biosensors can be applied to other biosensors for cancer or other diseases.

No MeSH data available.


Related in: MedlinePlus