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Detection of cardiac biomarkers using single polyaniline nanowire-based conductometric biosensors.

Lee I, Luo X, Huang J, Cui XT, Yun M - Biosensors (Basel) (2012)

Bottom Line: The single PANI nanowire-based biosensors displayed linear sensing profiles for concentrations ranging from hundreds (fg/mL) to tens (ng/mL).This single PANI nanowire-based biosensor demonstrated superior biosensing reliability with the feasibility of label free detection and improved processing cost efficiency due to good biocompatibility of PANI to monoclonal antibodies (mAbs).Therefore, this development of single PANI nanowire-based biosensors can be applied to other biosensors for cancer or other diseases.

View Article: PubMed Central - PubMed

Affiliation: Department of Electrical and Computer Engineering, University of Pittsburgh, Pittsburgh, PA 15261, USA. inl8@pitt.edu.

ABSTRACT
The detection of myoglobin (Myo), cardiac troponin I (cTnI), creatine kinase-MB (CK-MB), and b-type natriuretic peptide (BNP) plays a vital role in diagnosing cardiovascular diseases. Here we present single site-specific polyaniline (PANI) nanowire biosensors that can detect cardiac biomarkers such as Myo, cTnI, CK-MB, and BNP with ultra-high sensitivity and good specificity. Using single PANI nanowire-based biosensors integrated with microfluidic channels, very low concentrations of Myo (100 pg/mL), cTnI (250 fg/mL), CK-MB (150 fg/mL), and BNP (50 fg/mL) were detected. The single PANI nanowire-based biosensors displayed linear sensing profiles for concentrations ranging from hundreds (fg/mL) to tens (ng/mL). In addition, devices showed a fast (few minutes) response satisfying respective reference conditions for Myo, cTnI, CK-MB, and BNP diagnosis of heart failure and for determining the stage of the disease. This single PANI nanowire-based biosensor demonstrated superior biosensing reliability with the feasibility of label free detection and improved processing cost efficiency due to good biocompatibility of PANI to monoclonal antibodies (mAbs). Therefore, this development of single PANI nanowire-based biosensors can be applied to other biosensors for cancer or other diseases.

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Scanning electron microscopy (SEM) images of single PANI nanowires. (a) before the surface functionalization and (b) after the surface functionalization with cTnI mAbs. The two SEM images were taken at the same location of the nanowire.
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biosensors-02-00205-f002: Scanning electron microscopy (SEM) images of single PANI nanowires. (a) before the surface functionalization and (b) after the surface functionalization with cTnI mAbs. The two SEM images were taken at the same location of the nanowire.

Mentions: The same single PANI nanowires were compared by scanning electron microscopy (SEM) before and after the surface immobilization of mAbs to observe the change of nanowire surfaces as shown in Figure 2(a,b). In the SEM images, the observed difference of PANI nanowire surface distinguishes the functionalized nanowire from the non-functionalized nanowire. Before the functionalization, the single nanowire has a smooth surface and uniform dimension with a width of 100 nm as shown in Figure 2(a). In contrast, the surface of the functionalized single PANI nanowire shows a rough morphology with attached particles of 10–30 nm in diameter in Figure 2(b).


Detection of cardiac biomarkers using single polyaniline nanowire-based conductometric biosensors.

Lee I, Luo X, Huang J, Cui XT, Yun M - Biosensors (Basel) (2012)

Scanning electron microscopy (SEM) images of single PANI nanowires. (a) before the surface functionalization and (b) after the surface functionalization with cTnI mAbs. The two SEM images were taken at the same location of the nanowire.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263570&req=5

biosensors-02-00205-f002: Scanning electron microscopy (SEM) images of single PANI nanowires. (a) before the surface functionalization and (b) after the surface functionalization with cTnI mAbs. The two SEM images were taken at the same location of the nanowire.
Mentions: The same single PANI nanowires were compared by scanning electron microscopy (SEM) before and after the surface immobilization of mAbs to observe the change of nanowire surfaces as shown in Figure 2(a,b). In the SEM images, the observed difference of PANI nanowire surface distinguishes the functionalized nanowire from the non-functionalized nanowire. Before the functionalization, the single nanowire has a smooth surface and uniform dimension with a width of 100 nm as shown in Figure 2(a). In contrast, the surface of the functionalized single PANI nanowire shows a rough morphology with attached particles of 10–30 nm in diameter in Figure 2(b).

Bottom Line: The single PANI nanowire-based biosensors displayed linear sensing profiles for concentrations ranging from hundreds (fg/mL) to tens (ng/mL).This single PANI nanowire-based biosensor demonstrated superior biosensing reliability with the feasibility of label free detection and improved processing cost efficiency due to good biocompatibility of PANI to monoclonal antibodies (mAbs).Therefore, this development of single PANI nanowire-based biosensors can be applied to other biosensors for cancer or other diseases.

View Article: PubMed Central - PubMed

Affiliation: Department of Electrical and Computer Engineering, University of Pittsburgh, Pittsburgh, PA 15261, USA. inl8@pitt.edu.

ABSTRACT
The detection of myoglobin (Myo), cardiac troponin I (cTnI), creatine kinase-MB (CK-MB), and b-type natriuretic peptide (BNP) plays a vital role in diagnosing cardiovascular diseases. Here we present single site-specific polyaniline (PANI) nanowire biosensors that can detect cardiac biomarkers such as Myo, cTnI, CK-MB, and BNP with ultra-high sensitivity and good specificity. Using single PANI nanowire-based biosensors integrated with microfluidic channels, very low concentrations of Myo (100 pg/mL), cTnI (250 fg/mL), CK-MB (150 fg/mL), and BNP (50 fg/mL) were detected. The single PANI nanowire-based biosensors displayed linear sensing profiles for concentrations ranging from hundreds (fg/mL) to tens (ng/mL). In addition, devices showed a fast (few minutes) response satisfying respective reference conditions for Myo, cTnI, CK-MB, and BNP diagnosis of heart failure and for determining the stage of the disease. This single PANI nanowire-based biosensor demonstrated superior biosensing reliability with the feasibility of label free detection and improved processing cost efficiency due to good biocompatibility of PANI to monoclonal antibodies (mAbs). Therefore, this development of single PANI nanowire-based biosensors can be applied to other biosensors for cancer or other diseases.

No MeSH data available.


Related in: MedlinePlus