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Detection of cardiac biomarkers using single polyaniline nanowire-based conductometric biosensors.

Lee I, Luo X, Huang J, Cui XT, Yun M - Biosensors (Basel) (2012)

Bottom Line: The single PANI nanowire-based biosensors displayed linear sensing profiles for concentrations ranging from hundreds (fg/mL) to tens (ng/mL).This single PANI nanowire-based biosensor demonstrated superior biosensing reliability with the feasibility of label free detection and improved processing cost efficiency due to good biocompatibility of PANI to monoclonal antibodies (mAbs).Therefore, this development of single PANI nanowire-based biosensors can be applied to other biosensors for cancer or other diseases.

View Article: PubMed Central - PubMed

Affiliation: Department of Electrical and Computer Engineering, University of Pittsburgh, Pittsburgh, PA 15261, USA. inl8@pitt.edu.

ABSTRACT
The detection of myoglobin (Myo), cardiac troponin I (cTnI), creatine kinase-MB (CK-MB), and b-type natriuretic peptide (BNP) plays a vital role in diagnosing cardiovascular diseases. Here we present single site-specific polyaniline (PANI) nanowire biosensors that can detect cardiac biomarkers such as Myo, cTnI, CK-MB, and BNP with ultra-high sensitivity and good specificity. Using single PANI nanowire-based biosensors integrated with microfluidic channels, very low concentrations of Myo (100 pg/mL), cTnI (250 fg/mL), CK-MB (150 fg/mL), and BNP (50 fg/mL) were detected. The single PANI nanowire-based biosensors displayed linear sensing profiles for concentrations ranging from hundreds (fg/mL) to tens (ng/mL). In addition, devices showed a fast (few minutes) response satisfying respective reference conditions for Myo, cTnI, CK-MB, and BNP diagnosis of heart failure and for determining the stage of the disease. This single PANI nanowire-based biosensor demonstrated superior biosensing reliability with the feasibility of label free detection and improved processing cost efficiency due to good biocompatibility of PANI to monoclonal antibodies (mAbs). Therefore, this development of single PANI nanowire-based biosensors can be applied to other biosensors for cancer or other diseases.

No MeSH data available.


Related in: MedlinePlus

An illustration and the experimental set-up of the single polyaniline (PANI) nanowire biosensor to detect cardiac biomarkers. (a) The experimental setup; the microfluidic channel is adhered on the nanowire biosensor and the nanowire biosensor chip is mounted on a probe station connected to the semiconductor analyzer and syringe pump with inlet and outlet; (b) The conductance change in the single PANI nanowire-based biosensor is monitored. The injection of PBS (mark a), BSA (mark b), and cardiac biomarker (mark c) shows the different changes of conductance.
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biosensors-02-00205-f001: An illustration and the experimental set-up of the single polyaniline (PANI) nanowire biosensor to detect cardiac biomarkers. (a) The experimental setup; the microfluidic channel is adhered on the nanowire biosensor and the nanowire biosensor chip is mounted on a probe station connected to the semiconductor analyzer and syringe pump with inlet and outlet; (b) The conductance change in the single PANI nanowire-based biosensor is monitored. The injection of PBS (mark a), BSA (mark b), and cardiac biomarker (mark c) shows the different changes of conductance.

Mentions: A microfluidic channel was fabricated using polydimethylsiloxane (PDMS, Sylgard 184, Dow Corning Corp.) and negative photoresist (SU-8 2050, MicroChem Corp.). A designed mold of the microfluidic channel was lithographically patterned and developed on a Si/SiO2 wafer with spin-coated SU-8 2050 of 100 µm thickness. The fabricated microfluidic channels are 700 µm in width, 100 µm in height, and 4 mm in length and these dimensions are determined by the diameters of fluidic tube and syringe needle. The prepared PDMS was poured on the mold of the microfluidic channel and cured in an oven at 80 °C for 45 min. The fabricated PDMS microfluidic channel was adhered to a nanowire biosensor chip after O2 plasma treatment (250 mT, 30 W, 30 s) as shown in the inset of Figure 1(a). The single PANI nanowire biosensor integrated with the microfluidic channel was tested by infusing PBS, BSA, or target solutions using a syringe pump with the flow rate of 0.03 mL/min.


Detection of cardiac biomarkers using single polyaniline nanowire-based conductometric biosensors.

Lee I, Luo X, Huang J, Cui XT, Yun M - Biosensors (Basel) (2012)

An illustration and the experimental set-up of the single polyaniline (PANI) nanowire biosensor to detect cardiac biomarkers. (a) The experimental setup; the microfluidic channel is adhered on the nanowire biosensor and the nanowire biosensor chip is mounted on a probe station connected to the semiconductor analyzer and syringe pump with inlet and outlet; (b) The conductance change in the single PANI nanowire-based biosensor is monitored. The injection of PBS (mark a), BSA (mark b), and cardiac biomarker (mark c) shows the different changes of conductance.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263570&req=5

biosensors-02-00205-f001: An illustration and the experimental set-up of the single polyaniline (PANI) nanowire biosensor to detect cardiac biomarkers. (a) The experimental setup; the microfluidic channel is adhered on the nanowire biosensor and the nanowire biosensor chip is mounted on a probe station connected to the semiconductor analyzer and syringe pump with inlet and outlet; (b) The conductance change in the single PANI nanowire-based biosensor is monitored. The injection of PBS (mark a), BSA (mark b), and cardiac biomarker (mark c) shows the different changes of conductance.
Mentions: A microfluidic channel was fabricated using polydimethylsiloxane (PDMS, Sylgard 184, Dow Corning Corp.) and negative photoresist (SU-8 2050, MicroChem Corp.). A designed mold of the microfluidic channel was lithographically patterned and developed on a Si/SiO2 wafer with spin-coated SU-8 2050 of 100 µm thickness. The fabricated microfluidic channels are 700 µm in width, 100 µm in height, and 4 mm in length and these dimensions are determined by the diameters of fluidic tube and syringe needle. The prepared PDMS was poured on the mold of the microfluidic channel and cured in an oven at 80 °C for 45 min. The fabricated PDMS microfluidic channel was adhered to a nanowire biosensor chip after O2 plasma treatment (250 mT, 30 W, 30 s) as shown in the inset of Figure 1(a). The single PANI nanowire biosensor integrated with the microfluidic channel was tested by infusing PBS, BSA, or target solutions using a syringe pump with the flow rate of 0.03 mL/min.

Bottom Line: The single PANI nanowire-based biosensors displayed linear sensing profiles for concentrations ranging from hundreds (fg/mL) to tens (ng/mL).This single PANI nanowire-based biosensor demonstrated superior biosensing reliability with the feasibility of label free detection and improved processing cost efficiency due to good biocompatibility of PANI to monoclonal antibodies (mAbs).Therefore, this development of single PANI nanowire-based biosensors can be applied to other biosensors for cancer or other diseases.

View Article: PubMed Central - PubMed

Affiliation: Department of Electrical and Computer Engineering, University of Pittsburgh, Pittsburgh, PA 15261, USA. inl8@pitt.edu.

ABSTRACT
The detection of myoglobin (Myo), cardiac troponin I (cTnI), creatine kinase-MB (CK-MB), and b-type natriuretic peptide (BNP) plays a vital role in diagnosing cardiovascular diseases. Here we present single site-specific polyaniline (PANI) nanowire biosensors that can detect cardiac biomarkers such as Myo, cTnI, CK-MB, and BNP with ultra-high sensitivity and good specificity. Using single PANI nanowire-based biosensors integrated with microfluidic channels, very low concentrations of Myo (100 pg/mL), cTnI (250 fg/mL), CK-MB (150 fg/mL), and BNP (50 fg/mL) were detected. The single PANI nanowire-based biosensors displayed linear sensing profiles for concentrations ranging from hundreds (fg/mL) to tens (ng/mL). In addition, devices showed a fast (few minutes) response satisfying respective reference conditions for Myo, cTnI, CK-MB, and BNP diagnosis of heart failure and for determining the stage of the disease. This single PANI nanowire-based biosensor demonstrated superior biosensing reliability with the feasibility of label free detection and improved processing cost efficiency due to good biocompatibility of PANI to monoclonal antibodies (mAbs). Therefore, this development of single PANI nanowire-based biosensors can be applied to other biosensors for cancer or other diseases.

No MeSH data available.


Related in: MedlinePlus