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Sensitive detection of capsaicinoids using a surface plasmon resonance sensor with anti-homovanillic Acid polyclonal antibodies.

Nakamura S, Yatabe R, Onodera T, Toko K - Biosensors (Basel) (2013)

Bottom Line: An indirect competitive assay was carried out to detect capsaicinoids using SPR sensor chips on which different capsaicin analogues were immobilized.For the sensor chip on which 4-hydroxy-3-methoxybenzylamine hydrochloride was immobilized, a detection limit of 150 ppb was achieved.We found that the incubation time was not required and the detection can be completed in five minutes.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Information Science and Electrical Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan. nakamura@belab.ed.kyushu-u.ac.jp.

ABSTRACT
Recently, highly functional biosensors have been developed in preparation for possible large-scale terrorist attacks using chemical warfare agents. Practically applicable sensors are required to have various abilities, such as high portability and operability, the capability of performing rapid and continuous measurement, as well as high sensitivity and selectivity. We developed the detection method of capsaicinoids, the main component of some lachrymators, using a surface plasmon resonance (SPR) immunosensor as an on-site detection sensor. Homovanillic acid, which has a vanillyl group similar to capsaicinoids such as capsaicin and dihydrocapsaicin, was bound to Concholepas concholepas hemocyanin (CCH) for use as an immunogen to generate polyclonal antibodies. An indirect competitive assay was carried out to detect capsaicinoids using SPR sensor chips on which different capsaicin analogues were immobilized. For the sensor chip on which 4-hydroxy-3-methoxybenzylamine hydrochloride was immobilized, a detection limit of 150 ppb was achieved. We found that the incubation time was not required and the detection can be completed in five minutes.

No MeSH data available.


Response characteristic to capsaicinoids on HMB modified chip.
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biosensors-03-00374-f003: Response characteristic to capsaicinoids on HMB modified chip.

Mentions: We constructed a calibration curve by carrying out an experiment on capsaicinoid detection using the sensor chip on which HMB was immobilized. Figure 3(a) shows the average with standard deviation of the three measurements. As shown in the figure, the bound percentage decreased with increasing capsaicinoid concentration. This is because the binding of the antibodies to the chip surface was inhibited by capsaicinoids, meaning that this sensor chip successfully detected capsaicinoids. The detection limit was 150 ppb in this measurement.


Sensitive detection of capsaicinoids using a surface plasmon resonance sensor with anti-homovanillic Acid polyclonal antibodies.

Nakamura S, Yatabe R, Onodera T, Toko K - Biosensors (Basel) (2013)

Response characteristic to capsaicinoids on HMB modified chip.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263568&req=5

biosensors-03-00374-f003: Response characteristic to capsaicinoids on HMB modified chip.
Mentions: We constructed a calibration curve by carrying out an experiment on capsaicinoid detection using the sensor chip on which HMB was immobilized. Figure 3(a) shows the average with standard deviation of the three measurements. As shown in the figure, the bound percentage decreased with increasing capsaicinoid concentration. This is because the binding of the antibodies to the chip surface was inhibited by capsaicinoids, meaning that this sensor chip successfully detected capsaicinoids. The detection limit was 150 ppb in this measurement.

Bottom Line: An indirect competitive assay was carried out to detect capsaicinoids using SPR sensor chips on which different capsaicin analogues were immobilized.For the sensor chip on which 4-hydroxy-3-methoxybenzylamine hydrochloride was immobilized, a detection limit of 150 ppb was achieved.We found that the incubation time was not required and the detection can be completed in five minutes.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Information Science and Electrical Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan. nakamura@belab.ed.kyushu-u.ac.jp.

ABSTRACT
Recently, highly functional biosensors have been developed in preparation for possible large-scale terrorist attacks using chemical warfare agents. Practically applicable sensors are required to have various abilities, such as high portability and operability, the capability of performing rapid and continuous measurement, as well as high sensitivity and selectivity. We developed the detection method of capsaicinoids, the main component of some lachrymators, using a surface plasmon resonance (SPR) immunosensor as an on-site detection sensor. Homovanillic acid, which has a vanillyl group similar to capsaicinoids such as capsaicin and dihydrocapsaicin, was bound to Concholepas concholepas hemocyanin (CCH) for use as an immunogen to generate polyclonal antibodies. An indirect competitive assay was carried out to detect capsaicinoids using SPR sensor chips on which different capsaicin analogues were immobilized. For the sensor chip on which 4-hydroxy-3-methoxybenzylamine hydrochloride was immobilized, a detection limit of 150 ppb was achieved. We found that the incubation time was not required and the detection can be completed in five minutes.

No MeSH data available.