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Sensitive detection of capsaicinoids using a surface plasmon resonance sensor with anti-homovanillic Acid polyclonal antibodies.

Nakamura S, Yatabe R, Onodera T, Toko K - Biosensors (Basel) (2013)

Bottom Line: An indirect competitive assay was carried out to detect capsaicinoids using SPR sensor chips on which different capsaicin analogues were immobilized.For the sensor chip on which 4-hydroxy-3-methoxybenzylamine hydrochloride was immobilized, a detection limit of 150 ppb was achieved.We found that the incubation time was not required and the detection can be completed in five minutes.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Information Science and Electrical Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan. nakamura@belab.ed.kyushu-u.ac.jp.

ABSTRACT
Recently, highly functional biosensors have been developed in preparation for possible large-scale terrorist attacks using chemical warfare agents. Practically applicable sensors are required to have various abilities, such as high portability and operability, the capability of performing rapid and continuous measurement, as well as high sensitivity and selectivity. We developed the detection method of capsaicinoids, the main component of some lachrymators, using a surface plasmon resonance (SPR) immunosensor as an on-site detection sensor. Homovanillic acid, which has a vanillyl group similar to capsaicinoids such as capsaicin and dihydrocapsaicin, was bound to Concholepas concholepas hemocyanin (CCH) for use as an immunogen to generate polyclonal antibodies. An indirect competitive assay was carried out to detect capsaicinoids using SPR sensor chips on which different capsaicin analogues were immobilized. For the sensor chip on which 4-hydroxy-3-methoxybenzylamine hydrochloride was immobilized, a detection limit of 150 ppb was achieved. We found that the incubation time was not required and the detection can be completed in five minutes.

No MeSH data available.


Surface plasmon resonance (SPR) sensorgrams of indirect competitive assay on HMB chip using 25 ppm anti-HVA polyclonal antibodies for detection of 1 ppm capsaicinoids.
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biosensors-03-00374-f002: Surface plasmon resonance (SPR) sensorgrams of indirect competitive assay on HMB chip using 25 ppm anti-HVA polyclonal antibodies for detection of 1 ppm capsaicinoids.

Mentions: Figure 2 shows a sensorgram obtained by this measurement on HMB chip. The sensor response sharply changes immediately after the start of the injection and immediately before the end of the injection because of the bulk effect (change in the refractive index of the solvent) caused by the injection of an ethanol-containing solution, which is not related to the binding of the antibody to the sensor surface. To adjust the effect of ethanol among the solutions with different capsaicinoids concentrations, ethanol was added to the reference antibody solution in accordance with the dilution rate of capsaicinoids so that each solution contained an equivalent concentration of ethanol. When a low-concentration target is subjected to indirect competitive assay, the concentration of antibodies in the solution becomes relatively high to target substances. The concentration may decrease the change in the bound percentage and therefore decrease the sensitivity. Hence, it is desirable to decrease the concentration of the antibody solution to as low as possible. For this reason, the final concentration of the antibody solution was set to 25 ppm, at which the sensor response of approximately 100 RU was obtained.


Sensitive detection of capsaicinoids using a surface plasmon resonance sensor with anti-homovanillic Acid polyclonal antibodies.

Nakamura S, Yatabe R, Onodera T, Toko K - Biosensors (Basel) (2013)

Surface plasmon resonance (SPR) sensorgrams of indirect competitive assay on HMB chip using 25 ppm anti-HVA polyclonal antibodies for detection of 1 ppm capsaicinoids.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263568&req=5

biosensors-03-00374-f002: Surface plasmon resonance (SPR) sensorgrams of indirect competitive assay on HMB chip using 25 ppm anti-HVA polyclonal antibodies for detection of 1 ppm capsaicinoids.
Mentions: Figure 2 shows a sensorgram obtained by this measurement on HMB chip. The sensor response sharply changes immediately after the start of the injection and immediately before the end of the injection because of the bulk effect (change in the refractive index of the solvent) caused by the injection of an ethanol-containing solution, which is not related to the binding of the antibody to the sensor surface. To adjust the effect of ethanol among the solutions with different capsaicinoids concentrations, ethanol was added to the reference antibody solution in accordance with the dilution rate of capsaicinoids so that each solution contained an equivalent concentration of ethanol. When a low-concentration target is subjected to indirect competitive assay, the concentration of antibodies in the solution becomes relatively high to target substances. The concentration may decrease the change in the bound percentage and therefore decrease the sensitivity. Hence, it is desirable to decrease the concentration of the antibody solution to as low as possible. For this reason, the final concentration of the antibody solution was set to 25 ppm, at which the sensor response of approximately 100 RU was obtained.

Bottom Line: An indirect competitive assay was carried out to detect capsaicinoids using SPR sensor chips on which different capsaicin analogues were immobilized.For the sensor chip on which 4-hydroxy-3-methoxybenzylamine hydrochloride was immobilized, a detection limit of 150 ppb was achieved.We found that the incubation time was not required and the detection can be completed in five minutes.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Information Science and Electrical Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan. nakamura@belab.ed.kyushu-u.ac.jp.

ABSTRACT
Recently, highly functional biosensors have been developed in preparation for possible large-scale terrorist attacks using chemical warfare agents. Practically applicable sensors are required to have various abilities, such as high portability and operability, the capability of performing rapid and continuous measurement, as well as high sensitivity and selectivity. We developed the detection method of capsaicinoids, the main component of some lachrymators, using a surface plasmon resonance (SPR) immunosensor as an on-site detection sensor. Homovanillic acid, which has a vanillyl group similar to capsaicinoids such as capsaicin and dihydrocapsaicin, was bound to Concholepas concholepas hemocyanin (CCH) for use as an immunogen to generate polyclonal antibodies. An indirect competitive assay was carried out to detect capsaicinoids using SPR sensor chips on which different capsaicin analogues were immobilized. For the sensor chip on which 4-hydroxy-3-methoxybenzylamine hydrochloride was immobilized, a detection limit of 150 ppb was achieved. We found that the incubation time was not required and the detection can be completed in five minutes.

No MeSH data available.