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Sensitive detection of capsaicinoids using a surface plasmon resonance sensor with anti-homovanillic Acid polyclonal antibodies.

Nakamura S, Yatabe R, Onodera T, Toko K - Biosensors (Basel) (2013)

Bottom Line: An indirect competitive assay was carried out to detect capsaicinoids using SPR sensor chips on which different capsaicin analogues were immobilized.For the sensor chip on which 4-hydroxy-3-methoxybenzylamine hydrochloride was immobilized, a detection limit of 150 ppb was achieved.We found that the incubation time was not required and the detection can be completed in five minutes.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Information Science and Electrical Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan. nakamura@belab.ed.kyushu-u.ac.jp.

ABSTRACT
Recently, highly functional biosensors have been developed in preparation for possible large-scale terrorist attacks using chemical warfare agents. Practically applicable sensors are required to have various abilities, such as high portability and operability, the capability of performing rapid and continuous measurement, as well as high sensitivity and selectivity. We developed the detection method of capsaicinoids, the main component of some lachrymators, using a surface plasmon resonance (SPR) immunosensor as an on-site detection sensor. Homovanillic acid, which has a vanillyl group similar to capsaicinoids such as capsaicin and dihydrocapsaicin, was bound to Concholepas concholepas hemocyanin (CCH) for use as an immunogen to generate polyclonal antibodies. An indirect competitive assay was carried out to detect capsaicinoids using SPR sensor chips on which different capsaicin analogues were immobilized. For the sensor chip on which 4-hydroxy-3-methoxybenzylamine hydrochloride was immobilized, a detection limit of 150 ppb was achieved. We found that the incubation time was not required and the detection can be completed in five minutes.

No MeSH data available.


Structures of capsaicin and capsaicin-analogues.
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Related In: Results  -  Collection

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biosensors-03-00374-f001: Structures of capsaicin and capsaicin-analogues.

Mentions: Three sensor chips with different capsaicin analogues immobilized were prepared to detect capsaicinoids. Figure 1 shows the chemical structures of capsaicin and its analogues. The capsaicin analogues have a vanillyl group similar to capsaicin. It is known that oligo (ethylene glycol)-terminated SAM has a property which prevents nonspecific adsorptions of proteins [11,15,19]. When 100 ppm antibody solution was injected into the sensor chips, binding of the antibody to the three sensor chips was observed.


Sensitive detection of capsaicinoids using a surface plasmon resonance sensor with anti-homovanillic Acid polyclonal antibodies.

Nakamura S, Yatabe R, Onodera T, Toko K - Biosensors (Basel) (2013)

Structures of capsaicin and capsaicin-analogues.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263568&req=5

biosensors-03-00374-f001: Structures of capsaicin and capsaicin-analogues.
Mentions: Three sensor chips with different capsaicin analogues immobilized were prepared to detect capsaicinoids. Figure 1 shows the chemical structures of capsaicin and its analogues. The capsaicin analogues have a vanillyl group similar to capsaicin. It is known that oligo (ethylene glycol)-terminated SAM has a property which prevents nonspecific adsorptions of proteins [11,15,19]. When 100 ppm antibody solution was injected into the sensor chips, binding of the antibody to the three sensor chips was observed.

Bottom Line: An indirect competitive assay was carried out to detect capsaicinoids using SPR sensor chips on which different capsaicin analogues were immobilized.For the sensor chip on which 4-hydroxy-3-methoxybenzylamine hydrochloride was immobilized, a detection limit of 150 ppb was achieved.We found that the incubation time was not required and the detection can be completed in five minutes.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Information Science and Electrical Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan. nakamura@belab.ed.kyushu-u.ac.jp.

ABSTRACT
Recently, highly functional biosensors have been developed in preparation for possible large-scale terrorist attacks using chemical warfare agents. Practically applicable sensors are required to have various abilities, such as high portability and operability, the capability of performing rapid and continuous measurement, as well as high sensitivity and selectivity. We developed the detection method of capsaicinoids, the main component of some lachrymators, using a surface plasmon resonance (SPR) immunosensor as an on-site detection sensor. Homovanillic acid, which has a vanillyl group similar to capsaicinoids such as capsaicin and dihydrocapsaicin, was bound to Concholepas concholepas hemocyanin (CCH) for use as an immunogen to generate polyclonal antibodies. An indirect competitive assay was carried out to detect capsaicinoids using SPR sensor chips on which different capsaicin analogues were immobilized. For the sensor chip on which 4-hydroxy-3-methoxybenzylamine hydrochloride was immobilized, a detection limit of 150 ppb was achieved. We found that the incubation time was not required and the detection can be completed in five minutes.

No MeSH data available.