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Opening Study on the Development of a New Biosensor for Metal Toxicity Based on Pseudomonas fluorescens Pyoverdine.

Chiadò A, Varani L, Bosco F, Marmo L - Biosensors (Basel) (2013)

Bottom Line: To date, different kinds of biosensing elements have been used effectively for environmental monitoring.Each of these variables has been shown to influence the synthesis of siderophore: for instance, the lower the temperature, the higher the production of pyoverdine.Moreover, the concentration of pyoverdine produced in the presence of metals has been compared with the maximum allowable concentrations indicated in international regulations (e.g., 98/83/EC), and a correlation that could be useful to build a colorimetric biosensor has been observed.

View Article: PubMed Central - PubMed

Affiliation: Department of Applied Science and Technology, Politecnico di Torino, Corso Duca degli Abruzzi, 24, 10129, Torino, Italy. alessandro.chiado@polito.it.

ABSTRACT
To date, different kinds of biosensing elements have been used effectively for environmental monitoring. Microbial cells seem to be well-suited for this task: they are cheap, adaptable to variable field conditions and give a measurable response to a broad number of chemicals. Among different pollutants, heavy metals are still a major problem for the environment. A reasonable starting point for the selection of a biorecognition element to develop a biosensor for metals could be that of a microorganism that exhibits good mechanisms to cope with metals. Pseudomonads are characterized by the secretion of siderophores (e.g., pyoverdine), low-molecular weight compounds that chelate Fe3+ during iron starvation. Pyoverdine is easily detected by colorimetric assay, and it is suitable for simple online measurements. In this work, in order to evaluate pyoverdine as a biorecognition element for metal detection, the influence of metal ions (Fe3+, Cu2+, Zn2+), but also of temperature, pH and nutrients, on microbial growth and pyoverdine regulation has been studied in P. fluorescens. Each of these variables has been shown to influence the synthesis of siderophore: for instance, the lower the temperature, the higher the production of pyoverdine. Moreover, the concentration of pyoverdine produced in the presence of metals has been compared with the maximum allowable concentrations indicated in international regulations (e.g., 98/83/EC), and a correlation that could be useful to build a colorimetric biosensor has been observed.

No MeSH data available.


Related in: MedlinePlus

(a) Influence of different C sources on biomass growth and pH behavior; (b) influence of different C sources on pyoverdine production. OD, optical density.
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biosensors-03-00385-f001: (a) Influence of different C sources on biomass growth and pH behavior; (b) influence of different C sources on pyoverdine production. OD, optical density.

Mentions: The pyoverdine production in P. fluorescens seems to be influenced by different carbon sources [23]. Cultures with glucose and succinic acid, by far the most commonly used to culture this strain, were set up to establish the best C source for the pyoverdine synthesis. Both types of culture showed similar growth behavior (OD620) (Figure 1(a)), with the siderophore content increasing along with the biomass concentration, from the early exponential growth phase until the culture entered the stationary one. Nevertheless, succinic acid produced more pyoverdine (nearly double) than glucose did (Figure 1(b)), even though the C/N ratio was unchanged (17.6 for glucose and 17.9 for succinic acid).


Opening Study on the Development of a New Biosensor for Metal Toxicity Based on Pseudomonas fluorescens Pyoverdine.

Chiadò A, Varani L, Bosco F, Marmo L - Biosensors (Basel) (2013)

(a) Influence of different C sources on biomass growth and pH behavior; (b) influence of different C sources on pyoverdine production. OD, optical density.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263567&req=5

biosensors-03-00385-f001: (a) Influence of different C sources on biomass growth and pH behavior; (b) influence of different C sources on pyoverdine production. OD, optical density.
Mentions: The pyoverdine production in P. fluorescens seems to be influenced by different carbon sources [23]. Cultures with glucose and succinic acid, by far the most commonly used to culture this strain, were set up to establish the best C source for the pyoverdine synthesis. Both types of culture showed similar growth behavior (OD620) (Figure 1(a)), with the siderophore content increasing along with the biomass concentration, from the early exponential growth phase until the culture entered the stationary one. Nevertheless, succinic acid produced more pyoverdine (nearly double) than glucose did (Figure 1(b)), even though the C/N ratio was unchanged (17.6 for glucose and 17.9 for succinic acid).

Bottom Line: To date, different kinds of biosensing elements have been used effectively for environmental monitoring.Each of these variables has been shown to influence the synthesis of siderophore: for instance, the lower the temperature, the higher the production of pyoverdine.Moreover, the concentration of pyoverdine produced in the presence of metals has been compared with the maximum allowable concentrations indicated in international regulations (e.g., 98/83/EC), and a correlation that could be useful to build a colorimetric biosensor has been observed.

View Article: PubMed Central - PubMed

Affiliation: Department of Applied Science and Technology, Politecnico di Torino, Corso Duca degli Abruzzi, 24, 10129, Torino, Italy. alessandro.chiado@polito.it.

ABSTRACT
To date, different kinds of biosensing elements have been used effectively for environmental monitoring. Microbial cells seem to be well-suited for this task: they are cheap, adaptable to variable field conditions and give a measurable response to a broad number of chemicals. Among different pollutants, heavy metals are still a major problem for the environment. A reasonable starting point for the selection of a biorecognition element to develop a biosensor for metals could be that of a microorganism that exhibits good mechanisms to cope with metals. Pseudomonads are characterized by the secretion of siderophores (e.g., pyoverdine), low-molecular weight compounds that chelate Fe3+ during iron starvation. Pyoverdine is easily detected by colorimetric assay, and it is suitable for simple online measurements. In this work, in order to evaluate pyoverdine as a biorecognition element for metal detection, the influence of metal ions (Fe3+, Cu2+, Zn2+), but also of temperature, pH and nutrients, on microbial growth and pyoverdine regulation has been studied in P. fluorescens. Each of these variables has been shown to influence the synthesis of siderophore: for instance, the lower the temperature, the higher the production of pyoverdine. Moreover, the concentration of pyoverdine produced in the presence of metals has been compared with the maximum allowable concentrations indicated in international regulations (e.g., 98/83/EC), and a correlation that could be useful to build a colorimetric biosensor has been observed.

No MeSH data available.


Related in: MedlinePlus