Limits...
A low-cost, high-performance system for fluorescence lateral flow assays.

Lee LG, Nordman ES, Johnson MD, Oldham MF - Biosensors (Basel) (2013)

Bottom Line: A wide range of values for the ratio of signal to nonspecific binding was found, from 50 for R-phycoerythrin (R-PE) to 0.15 for Brilliant Violet 605.Fluorescence detection with R-PE and absorbance detection with colloidal gold were directly compared in lateral flow using biotinylated bovine serum albumen (BSA) as the analyte.We believe our inexpensive yet high-performance platform will be useful for providing quantitative and sensitive detection in a point-of-care setting.

View Article: PubMed Central - PubMed

Affiliation: Song Diagnostic Research LLC, 1 Megans Lane, Woodside, CA 94062, USA. Linda@songdx.net.

ABSTRACT
We demonstrate a fluorescence lateral flow system that has excellent sensitivity and wide dynamic range. The illumination system utilizes an LED, plastic lenses and plastic and colored glass filters for the excitation and emission light. Images are collected on an iPhone 4. Several fluorescent dyes with long Stokes shifts were evaluated for their signal and nonspecific binding in lateral flow. A wide range of values for the ratio of signal to nonspecific binding was found, from 50 for R-phycoerythrin (R-PE) to 0.15 for Brilliant Violet 605. The long Stokes shift of R-PE allowed the use of inexpensive plastic filters rather than costly interference filters to block the LED light. Fluorescence detection with R-PE and absorbance detection with colloidal gold were directly compared in lateral flow using biotinylated bovine serum albumen (BSA) as the analyte. Fluorescence provided linear data over a range of 0.4-4,000 ng/mL with a 1,000-fold signal change while colloidal gold provided non-linear data over a range of 16-4,000 ng/mL with a 10-fold signal change. A comparison using human chorionic gonadotropin (hCG) as the analyte showed a similar advantage in the fluorescent system. We believe our inexpensive yet high-performance platform will be useful for providing quantitative and sensitive detection in a point-of-care setting.

No MeSH data available.


Fluorescence system concept and estimated cost. Functions include LFIA detection, analysis and communications. Cost of smartphone not included.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4263565&req=5

biosensors-03-00360-f005: Fluorescence system concept and estimated cost. Functions include LFIA detection, analysis and communications. Cost of smartphone not included.

Mentions: Our ultimate goal is to create a manufacturable reader system that would be an accessory to a smartphone. Shown in Figure 5 is an early concept of such a reader; we have estimated the final cost based on the component parts.


A low-cost, high-performance system for fluorescence lateral flow assays.

Lee LG, Nordman ES, Johnson MD, Oldham MF - Biosensors (Basel) (2013)

Fluorescence system concept and estimated cost. Functions include LFIA detection, analysis and communications. Cost of smartphone not included.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263565&req=5

biosensors-03-00360-f005: Fluorescence system concept and estimated cost. Functions include LFIA detection, analysis and communications. Cost of smartphone not included.
Mentions: Our ultimate goal is to create a manufacturable reader system that would be an accessory to a smartphone. Shown in Figure 5 is an early concept of such a reader; we have estimated the final cost based on the component parts.

Bottom Line: A wide range of values for the ratio of signal to nonspecific binding was found, from 50 for R-phycoerythrin (R-PE) to 0.15 for Brilliant Violet 605.Fluorescence detection with R-PE and absorbance detection with colloidal gold were directly compared in lateral flow using biotinylated bovine serum albumen (BSA) as the analyte.We believe our inexpensive yet high-performance platform will be useful for providing quantitative and sensitive detection in a point-of-care setting.

View Article: PubMed Central - PubMed

Affiliation: Song Diagnostic Research LLC, 1 Megans Lane, Woodside, CA 94062, USA. Linda@songdx.net.

ABSTRACT
We demonstrate a fluorescence lateral flow system that has excellent sensitivity and wide dynamic range. The illumination system utilizes an LED, plastic lenses and plastic and colored glass filters for the excitation and emission light. Images are collected on an iPhone 4. Several fluorescent dyes with long Stokes shifts were evaluated for their signal and nonspecific binding in lateral flow. A wide range of values for the ratio of signal to nonspecific binding was found, from 50 for R-phycoerythrin (R-PE) to 0.15 for Brilliant Violet 605. The long Stokes shift of R-PE allowed the use of inexpensive plastic filters rather than costly interference filters to block the LED light. Fluorescence detection with R-PE and absorbance detection with colloidal gold were directly compared in lateral flow using biotinylated bovine serum albumen (BSA) as the analyte. Fluorescence provided linear data over a range of 0.4-4,000 ng/mL with a 1,000-fold signal change while colloidal gold provided non-linear data over a range of 16-4,000 ng/mL with a 10-fold signal change. A comparison using human chorionic gonadotropin (hCG) as the analyte showed a similar advantage in the fluorescent system. We believe our inexpensive yet high-performance platform will be useful for providing quantitative and sensitive detection in a point-of-care setting.

No MeSH data available.