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A New Approach for Detection Improvement of the Creutzfeldt-Jakob Disorder through a Specific Surface Chemistry Applied onto Titration Well.

Mille C, Debarnot D, Zorzi W, Moualij BE, Quadrio I, Perret-Liaudet A, Coudreuse A, Legeay G, Poncin-Epaillard F - Biosensors (Basel) (2012)

Bottom Line: It is achieved thanks to the association of plasma chemistry and coating with different amphiphilic molecules bearing either ionic charges and/or long hydrocarbon chains.The treated support by 3-butenylamine hydrochloride improves the signal detection of recombinant protein, while surface modification with the 3,7-dimethylocta-2,6-dien-1-diamine (geranylamine) enhances the sensitivity of the native protein.Beside the surface chemistry effect, these different results are associated with protein conformation.

View Article: PubMed Central - PubMed

Affiliation: Université, UMR Université du Maine, CNRS n°6283, Institut des Molécules et Matériaux du Mans, Département Polymères, Colloïdes et Interfaces, av. O. Messiaen, 72085 Le Mans, France. caroline.mille.etu@univ-lemans.fr.

ABSTRACT
This work illustrates the enhancement of the sensitivity of the ELISA titration for recombinant human and native prion proteins, while reducing other non-specific adsorptions that could increase the background signal and lead to a low sensitivity and false positives. It is achieved thanks to the association of plasma chemistry and coating with different amphiphilic molecules bearing either ionic charges and/or long hydrocarbon chains. The treated support by 3-butenylamine hydrochloride improves the signal detection of recombinant protein, while surface modification with the 3,7-dimethylocta-2,6-dien-1-diamine (geranylamine) enhances the sensitivity of the native protein. Beside the surface chemistry effect, these different results are associated with protein conformation.

No MeSH data available.


Evaluation of non-specific associations for each surface treatment during PrPrechum ELISA titration.
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Related In: Results  -  Collection

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biosensors-02-00433-f006: Evaluation of non-specific associations for each surface treatment during PrPrechum ELISA titration.

Mentions: Figure 6 shows the values of optical density for PrPrechum titration recorded under different experimental conditions, where the antigen, capture and detection antibodies were each in turn removed from the protocol.


A New Approach for Detection Improvement of the Creutzfeldt-Jakob Disorder through a Specific Surface Chemistry Applied onto Titration Well.

Mille C, Debarnot D, Zorzi W, Moualij BE, Quadrio I, Perret-Liaudet A, Coudreuse A, Legeay G, Poncin-Epaillard F - Biosensors (Basel) (2012)

Evaluation of non-specific associations for each surface treatment during PrPrechum ELISA titration.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263562&req=5

biosensors-02-00433-f006: Evaluation of non-specific associations for each surface treatment during PrPrechum ELISA titration.
Mentions: Figure 6 shows the values of optical density for PrPrechum titration recorded under different experimental conditions, where the antigen, capture and detection antibodies were each in turn removed from the protocol.

Bottom Line: It is achieved thanks to the association of plasma chemistry and coating with different amphiphilic molecules bearing either ionic charges and/or long hydrocarbon chains.The treated support by 3-butenylamine hydrochloride improves the signal detection of recombinant protein, while surface modification with the 3,7-dimethylocta-2,6-dien-1-diamine (geranylamine) enhances the sensitivity of the native protein.Beside the surface chemistry effect, these different results are associated with protein conformation.

View Article: PubMed Central - PubMed

Affiliation: Université, UMR Université du Maine, CNRS n°6283, Institut des Molécules et Matériaux du Mans, Département Polymères, Colloïdes et Interfaces, av. O. Messiaen, 72085 Le Mans, France. caroline.mille.etu@univ-lemans.fr.

ABSTRACT
This work illustrates the enhancement of the sensitivity of the ELISA titration for recombinant human and native prion proteins, while reducing other non-specific adsorptions that could increase the background signal and lead to a low sensitivity and false positives. It is achieved thanks to the association of plasma chemistry and coating with different amphiphilic molecules bearing either ionic charges and/or long hydrocarbon chains. The treated support by 3-butenylamine hydrochloride improves the signal detection of recombinant protein, while surface modification with the 3,7-dimethylocta-2,6-dien-1-diamine (geranylamine) enhances the sensitivity of the native protein. Beside the surface chemistry effect, these different results are associated with protein conformation.

No MeSH data available.