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A New Approach for Detection Improvement of the Creutzfeldt-Jakob Disorder through a Specific Surface Chemistry Applied onto Titration Well.

Mille C, Debarnot D, Zorzi W, Moualij BE, Quadrio I, Perret-Liaudet A, Coudreuse A, Legeay G, Poncin-Epaillard F - Biosensors (Basel) (2012)

Bottom Line: It is achieved thanks to the association of plasma chemistry and coating with different amphiphilic molecules bearing either ionic charges and/or long hydrocarbon chains.The treated support by 3-butenylamine hydrochloride improves the signal detection of recombinant protein, while surface modification with the 3,7-dimethylocta-2,6-dien-1-diamine (geranylamine) enhances the sensitivity of the native protein.Beside the surface chemistry effect, these different results are associated with protein conformation.

View Article: PubMed Central - PubMed

Affiliation: Université, UMR Université du Maine, CNRS n°6283, Institut des Molécules et Matériaux du Mans, Département Polymères, Colloïdes et Interfaces, av. O. Messiaen, 72085 Le Mans, France. caroline.mille.etu@univ-lemans.fr.

ABSTRACT
This work illustrates the enhancement of the sensitivity of the ELISA titration for recombinant human and native prion proteins, while reducing other non-specific adsorptions that could increase the background signal and lead to a low sensitivity and false positives. It is achieved thanks to the association of plasma chemistry and coating with different amphiphilic molecules bearing either ionic charges and/or long hydrocarbon chains. The treated support by 3-butenylamine hydrochloride improves the signal detection of recombinant protein, while surface modification with the 3,7-dimethylocta-2,6-dien-1-diamine (geranylamine) enhances the sensitivity of the native protein. Beside the surface chemistry effect, these different results are associated with protein conformation.

No MeSH data available.


Related in: MedlinePlus

Dependence of the detection of PrPrechum protein on its concentration and the well inner-surface nature ((capture antibody) = 10 µg∙mL–1, (detection antibody) = 1 µg∙mL–1).
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biosensors-02-00433-f001: Dependence of the detection of PrPrechum protein on its concentration and the well inner-surface nature ((capture antibody) = 10 µg∙mL–1, (detection antibody) = 1 µg∙mL–1).

Mentions: Figure 1 shows the evolution of the optical density obtained with virgin or coated (T1, T2 and T3) PP wells according to a range of increasing concentrations of recombinant prion protein in the presence of the Saf32 capture antibody (10 µg∙mL–1) and the 4F7biot biotinylated detection antibody (1 µg∙mL–1).


A New Approach for Detection Improvement of the Creutzfeldt-Jakob Disorder through a Specific Surface Chemistry Applied onto Titration Well.

Mille C, Debarnot D, Zorzi W, Moualij BE, Quadrio I, Perret-Liaudet A, Coudreuse A, Legeay G, Poncin-Epaillard F - Biosensors (Basel) (2012)

Dependence of the detection of PrPrechum protein on its concentration and the well inner-surface nature ((capture antibody) = 10 µg∙mL–1, (detection antibody) = 1 µg∙mL–1).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263562&req=5

biosensors-02-00433-f001: Dependence of the detection of PrPrechum protein on its concentration and the well inner-surface nature ((capture antibody) = 10 µg∙mL–1, (detection antibody) = 1 µg∙mL–1).
Mentions: Figure 1 shows the evolution of the optical density obtained with virgin or coated (T1, T2 and T3) PP wells according to a range of increasing concentrations of recombinant prion protein in the presence of the Saf32 capture antibody (10 µg∙mL–1) and the 4F7biot biotinylated detection antibody (1 µg∙mL–1).

Bottom Line: It is achieved thanks to the association of plasma chemistry and coating with different amphiphilic molecules bearing either ionic charges and/or long hydrocarbon chains.The treated support by 3-butenylamine hydrochloride improves the signal detection of recombinant protein, while surface modification with the 3,7-dimethylocta-2,6-dien-1-diamine (geranylamine) enhances the sensitivity of the native protein.Beside the surface chemistry effect, these different results are associated with protein conformation.

View Article: PubMed Central - PubMed

Affiliation: Université, UMR Université du Maine, CNRS n°6283, Institut des Molécules et Matériaux du Mans, Département Polymères, Colloïdes et Interfaces, av. O. Messiaen, 72085 Le Mans, France. caroline.mille.etu@univ-lemans.fr.

ABSTRACT
This work illustrates the enhancement of the sensitivity of the ELISA titration for recombinant human and native prion proteins, while reducing other non-specific adsorptions that could increase the background signal and lead to a low sensitivity and false positives. It is achieved thanks to the association of plasma chemistry and coating with different amphiphilic molecules bearing either ionic charges and/or long hydrocarbon chains. The treated support by 3-butenylamine hydrochloride improves the signal detection of recombinant protein, while surface modification with the 3,7-dimethylocta-2,6-dien-1-diamine (geranylamine) enhances the sensitivity of the native protein. Beside the surface chemistry effect, these different results are associated with protein conformation.

No MeSH data available.


Related in: MedlinePlus