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Using complementary acoustic and optical techniques for quantitative monitoring of biomolecular adsorption at interfaces.

Konradi R, Textor M, Reimhult E - Biosensors (Basel) (2012)

Bottom Line: In this tutorial review, different optical and acoustic evanescent techniques are used to illustrate how an understanding of the transducer principle of each technique can be exploited for further interpretation of hydrated and extended polymer and biological films.The case studies deal with representative examples of adsorption of protein films, polymer brushes and lipid membranes, and describe e.g., how to deal with strongly vs. weakly hydrated films, large conformational changes and ordered layers of biomolecules.The presented systems and methods are compared to other representative examples from the increasing literature on the subject.

View Article: PubMed Central - PubMed

Affiliation: BASF SE, Advanced Materials and Systems Research, D-67056 Ludwigshafen, Germany. rupert.konradi@basf.com.

ABSTRACT
The great wealth of different surface sensitive techniques used in biosensing, most of which claim to measure adsorbed mass, can at first glance look unnecessary. However, with each technique relying on a different transducer principle there is something to be gained from a comparison. In this tutorial review, different optical and acoustic evanescent techniques are used to illustrate how an understanding of the transducer principle of each technique can be exploited for further interpretation of hydrated and extended polymer and biological films. Some of the most commonly used surface sensitive biosensor techniques (quartz crystal microbalance, optical waveguide spectroscopy and surface plasmon resonance) are briefly described and five case studies are presented to illustrate how different biosensing techniques can and often should be combined. The case studies deal with representative examples of adsorption of protein films, polymer brushes and lipid membranes, and describe e.g., how to deal with strongly vs. weakly hydrated films, large conformational changes and ordered layers of biomolecules. The presented systems and methods are compared to other representative examples from the increasing literature on the subject.

No MeSH data available.


Poly(L-lysine)-graft-poly(2-methyl-2-oxazoline).
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biosensors-02-00341-f013: Poly(L-lysine)-graft-poly(2-methyl-2-oxazoline).

Mentions: For the first case study we will investigate ultrathin hydrophilic polymer adlayers with the aim of relating molecular architecture, water content and resistance to protein adsorption. Important properties to characterize towards this aim are the layer thickness and hydration, for which a combination of evanescent optical sensing and acoustic sensing is highly suited. As an example, we employed OWLS and QCM-D to study the surface self-assembly and subsequent exposure to human serum of graft copolymers consisting of a polycationic poly(L-lysine) (PLL) backbone and poly(2-methyl-2-oxazoline) (PMOXA) side-chains (PLL-g-PMOXA, see Scheme 1).


Using complementary acoustic and optical techniques for quantitative monitoring of biomolecular adsorption at interfaces.

Konradi R, Textor M, Reimhult E - Biosensors (Basel) (2012)

Poly(L-lysine)-graft-poly(2-methyl-2-oxazoline).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263558&req=5

biosensors-02-00341-f013: Poly(L-lysine)-graft-poly(2-methyl-2-oxazoline).
Mentions: For the first case study we will investigate ultrathin hydrophilic polymer adlayers with the aim of relating molecular architecture, water content and resistance to protein adsorption. Important properties to characterize towards this aim are the layer thickness and hydration, for which a combination of evanescent optical sensing and acoustic sensing is highly suited. As an example, we employed OWLS and QCM-D to study the surface self-assembly and subsequent exposure to human serum of graft copolymers consisting of a polycationic poly(L-lysine) (PLL) backbone and poly(2-methyl-2-oxazoline) (PMOXA) side-chains (PLL-g-PMOXA, see Scheme 1).

Bottom Line: In this tutorial review, different optical and acoustic evanescent techniques are used to illustrate how an understanding of the transducer principle of each technique can be exploited for further interpretation of hydrated and extended polymer and biological films.The case studies deal with representative examples of adsorption of protein films, polymer brushes and lipid membranes, and describe e.g., how to deal with strongly vs. weakly hydrated films, large conformational changes and ordered layers of biomolecules.The presented systems and methods are compared to other representative examples from the increasing literature on the subject.

View Article: PubMed Central - PubMed

Affiliation: BASF SE, Advanced Materials and Systems Research, D-67056 Ludwigshafen, Germany. rupert.konradi@basf.com.

ABSTRACT
The great wealth of different surface sensitive techniques used in biosensing, most of which claim to measure adsorbed mass, can at first glance look unnecessary. However, with each technique relying on a different transducer principle there is something to be gained from a comparison. In this tutorial review, different optical and acoustic evanescent techniques are used to illustrate how an understanding of the transducer principle of each technique can be exploited for further interpretation of hydrated and extended polymer and biological films. Some of the most commonly used surface sensitive biosensor techniques (quartz crystal microbalance, optical waveguide spectroscopy and surface plasmon resonance) are briefly described and five case studies are presented to illustrate how different biosensing techniques can and often should be combined. The case studies deal with representative examples of adsorption of protein films, polymer brushes and lipid membranes, and describe e.g., how to deal with strongly vs. weakly hydrated films, large conformational changes and ordered layers of biomolecules. The presented systems and methods are compared to other representative examples from the increasing literature on the subject.

No MeSH data available.