Limits...
Water-soluble electrospun nanofibers as a method for on-chip reagent storage.

Dai M, Jin S, Nugen SR - Biosensors (Basel) (2012)

Bottom Line: This work demonstrates the ability to electrospin reagents into water-soluble nanofibers resulting in a stable on-chip enzyme storage format.Immediately following electrospinning, the activity peak for the HRP decreased by approximately 20%.The ability to store enzymes and other reagents on-chip in a rapidly dispersible format could reduce the assay steps required of an operator to perform.

View Article: PubMed Central - PubMed

Affiliation: University of Massachusetts, 102 Holdsworth Way, Amherst, MA 01003, USA. mdai@foodsci.umass.edu.

ABSTRACT
This work demonstrates the ability to electrospin reagents into water-soluble nanofibers resulting in a stable on-chip enzyme storage format. Polyvinylpyrrolidone (PVP) nanofibers were spun with incorporation of the enzyme horseradish peroxidase (HRP). Scanning electron microscopy (SEM) of the spun nanofibers was used to confirm the non-woven structure which had an average diameter of 155 ± 34 nm. The HRP containing fibers were tested for their change in activity following electrospinning and during storage. A colorimetric assay was used to characterize the activity of HRP by reaction with the nanofiber mats in a microtiter plate and monitoring the change in absorption over time. Immediately following electrospinning, the activity peak for the HRP decreased by approximately 20%. After a storage study over 280 days, 40% of the activity remained. In addition to activity, the fibers were observed to solubilize in the microfluidic chamber. The chromogenic 3,3',5,5'-tetramethylbenzidine solution reacted immediately with the fibers as they passed through a microfluidic channel. The ability to store enzymes and other reagents on-chip in a rapidly dispersible format could reduce the assay steps required of an operator to perform.

No MeSH data available.


The scanning electron microscopy (SEM) image of polyvinylpyrrolidone (PVP) nanofibers electrospun from 15 wt% PVP, 5 wt% wt sucrose in 0.1mg/mL horseradish peroxidase (HRP) water solution at a gap distance of 12cm with an applied voltage of 20kV.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4263557&req=5

biosensors-02-00388-f001: The scanning electron microscopy (SEM) image of polyvinylpyrrolidone (PVP) nanofibers electrospun from 15 wt% PVP, 5 wt% wt sucrose in 0.1mg/mL horseradish peroxidase (HRP) water solution at a gap distance of 12cm with an applied voltage of 20kV.

Mentions: Following electrospinning for 1 h, nanofiber mats of approximately 2 cm diameter were removed from the copper collection plate. The individual PVP fibers containing sucrose and HRP had an average diameter of 155 ± 34 nm (Figure 1). The addition of up to 10% (wt/v) sucrose and 1% (wt/v) protein did not have an effect on the morphology of the nanofibers.


Water-soluble electrospun nanofibers as a method for on-chip reagent storage.

Dai M, Jin S, Nugen SR - Biosensors (Basel) (2012)

The scanning electron microscopy (SEM) image of polyvinylpyrrolidone (PVP) nanofibers electrospun from 15 wt% PVP, 5 wt% wt sucrose in 0.1mg/mL horseradish peroxidase (HRP) water solution at a gap distance of 12cm with an applied voltage of 20kV.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263557&req=5

biosensors-02-00388-f001: The scanning electron microscopy (SEM) image of polyvinylpyrrolidone (PVP) nanofibers electrospun from 15 wt% PVP, 5 wt% wt sucrose in 0.1mg/mL horseradish peroxidase (HRP) water solution at a gap distance of 12cm with an applied voltage of 20kV.
Mentions: Following electrospinning for 1 h, nanofiber mats of approximately 2 cm diameter were removed from the copper collection plate. The individual PVP fibers containing sucrose and HRP had an average diameter of 155 ± 34 nm (Figure 1). The addition of up to 10% (wt/v) sucrose and 1% (wt/v) protein did not have an effect on the morphology of the nanofibers.

Bottom Line: This work demonstrates the ability to electrospin reagents into water-soluble nanofibers resulting in a stable on-chip enzyme storage format.Immediately following electrospinning, the activity peak for the HRP decreased by approximately 20%.The ability to store enzymes and other reagents on-chip in a rapidly dispersible format could reduce the assay steps required of an operator to perform.

View Article: PubMed Central - PubMed

Affiliation: University of Massachusetts, 102 Holdsworth Way, Amherst, MA 01003, USA. mdai@foodsci.umass.edu.

ABSTRACT
This work demonstrates the ability to electrospin reagents into water-soluble nanofibers resulting in a stable on-chip enzyme storage format. Polyvinylpyrrolidone (PVP) nanofibers were spun with incorporation of the enzyme horseradish peroxidase (HRP). Scanning electron microscopy (SEM) of the spun nanofibers was used to confirm the non-woven structure which had an average diameter of 155 ± 34 nm. The HRP containing fibers were tested for their change in activity following electrospinning and during storage. A colorimetric assay was used to characterize the activity of HRP by reaction with the nanofiber mats in a microtiter plate and monitoring the change in absorption over time. Immediately following electrospinning, the activity peak for the HRP decreased by approximately 20%. After a storage study over 280 days, 40% of the activity remained. In addition to activity, the fibers were observed to solubilize in the microfluidic chamber. The chromogenic 3,3',5,5'-tetramethylbenzidine solution reacted immediately with the fibers as they passed through a microfluidic channel. The ability to store enzymes and other reagents on-chip in a rapidly dispersible format could reduce the assay steps required of an operator to perform.

No MeSH data available.