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Synthesis of a functionalized polypyrrole coated electrotextile for use in biosensors.

McGraw SK, Alocilja E, Senecal A, Senecal K - Biosensors (Basel) (2012)

Bottom Line: The effects of dopant inclusion and post-polymerization wash steps were also analyzed.The initial results show a nonwoven fiber matrix can be successfully coated in a conductive, functionalized polymer while still maintaining surface area and fiber durability.The immobilized avidin was then successfully used to capture biotin.

View Article: PubMed Central - PubMed

Affiliation: Biosystems and Agricultural Engineering, Michigan State University, 524 S. Shaw Lane, 115 Farrall Hall, East Lansing, MI 48824, USA. andre.g.senecal.civ@mail.mil.

ABSTRACT
An electrotextile with a biosensing focus composed of conductive polymer coated microfibers that contain functional attachment sites for biorecognition elements was developed. Experiments were conducted to select a compound with a pendant functional group for inclusion in the polymer, a fiber platform, and polymerization solvent. The effects of dopant inclusion and post-polymerization wash steps were also analyzed. Finally, the successful attachment of avidin, which was then used to capture biotin, to the electrotextile was achieved. The initial results show a nonwoven fiber matrix can be successfully coated in a conductive, functionalized polymer while still maintaining surface area and fiber durability. A polypropylene fiber platform with a conductive polypyrrole coating using iron (III) chloride as an oxidant, water as a solvent, and 5-sulfosalicylic acid as a dopant exhibited the best coating consistency, material durability, and lowest resistance. Biological attachment of avidin was achieved on the fibers through the inclusion of a carboxyl functional group via 3-thiopheneacetic acid in the monomer. The immobilized avidin was then successfully used to capture biotin. This was confirmed through the use of fluorescent quantum dots and confocal microscopy. A preliminary electrochemical experiment using avidin for biotin detection was conducted. This technology will be extremely useful in the formation of electrotextiles for use in biosensor systems.

No MeSH data available.


The structure of poly(pyrrole-3TAA).
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Related In: Results  -  Collection

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biosensors-02-00465-f008: The structure of poly(pyrrole-3TAA).

Mentions: The chemical structure of the poly(pyrrole-3TAA) copolymer can be seen in Scheme 1. Previous work has been done exploring the polymerization of pyrrole with additional molecules added to create a co-monomer in order to build biological receptor sites into the polymer. These include biotin [17], benzophenone [18], pyrrole-3-carboxylic acid (3-COOH) [19] and 3-thiopheneacetic acid (3TAA) [20]. The structure is the same as that published in Vaddiraju et al. [20], however because the deposition method is aqueous instead of oCVD there are differences in the coating thicknesses, morphologies, and conductivities. The addition of an organic acid dopant will also affect these parameters.


Synthesis of a functionalized polypyrrole coated electrotextile for use in biosensors.

McGraw SK, Alocilja E, Senecal A, Senecal K - Biosensors (Basel) (2012)

The structure of poly(pyrrole-3TAA).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263555&req=5

biosensors-02-00465-f008: The structure of poly(pyrrole-3TAA).
Mentions: The chemical structure of the poly(pyrrole-3TAA) copolymer can be seen in Scheme 1. Previous work has been done exploring the polymerization of pyrrole with additional molecules added to create a co-monomer in order to build biological receptor sites into the polymer. These include biotin [17], benzophenone [18], pyrrole-3-carboxylic acid (3-COOH) [19] and 3-thiopheneacetic acid (3TAA) [20]. The structure is the same as that published in Vaddiraju et al. [20], however because the deposition method is aqueous instead of oCVD there are differences in the coating thicknesses, morphologies, and conductivities. The addition of an organic acid dopant will also affect these parameters.

Bottom Line: The effects of dopant inclusion and post-polymerization wash steps were also analyzed.The initial results show a nonwoven fiber matrix can be successfully coated in a conductive, functionalized polymer while still maintaining surface area and fiber durability.The immobilized avidin was then successfully used to capture biotin.

View Article: PubMed Central - PubMed

Affiliation: Biosystems and Agricultural Engineering, Michigan State University, 524 S. Shaw Lane, 115 Farrall Hall, East Lansing, MI 48824, USA. andre.g.senecal.civ@mail.mil.

ABSTRACT
An electrotextile with a biosensing focus composed of conductive polymer coated microfibers that contain functional attachment sites for biorecognition elements was developed. Experiments were conducted to select a compound with a pendant functional group for inclusion in the polymer, a fiber platform, and polymerization solvent. The effects of dopant inclusion and post-polymerization wash steps were also analyzed. Finally, the successful attachment of avidin, which was then used to capture biotin, to the electrotextile was achieved. The initial results show a nonwoven fiber matrix can be successfully coated in a conductive, functionalized polymer while still maintaining surface area and fiber durability. A polypropylene fiber platform with a conductive polypyrrole coating using iron (III) chloride as an oxidant, water as a solvent, and 5-sulfosalicylic acid as a dopant exhibited the best coating consistency, material durability, and lowest resistance. Biological attachment of avidin was achieved on the fibers through the inclusion of a carboxyl functional group via 3-thiopheneacetic acid in the monomer. The immobilized avidin was then successfully used to capture biotin. This was confirmed through the use of fluorescent quantum dots and confocal microscopy. A preliminary electrochemical experiment using avidin for biotin detection was conducted. This technology will be extremely useful in the formation of electrotextiles for use in biosensor systems.

No MeSH data available.