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Synthesis of a functionalized polypyrrole coated electrotextile for use in biosensors.

McGraw SK, Alocilja E, Senecal A, Senecal K - Biosensors (Basel) (2012)

Bottom Line: The effects of dopant inclusion and post-polymerization wash steps were also analyzed.The initial results show a nonwoven fiber matrix can be successfully coated in a conductive, functionalized polymer while still maintaining surface area and fiber durability.The immobilized avidin was then successfully used to capture biotin.

View Article: PubMed Central - PubMed

Affiliation: Biosystems and Agricultural Engineering, Michigan State University, 524 S. Shaw Lane, 115 Farrall Hall, East Lansing, MI 48824, USA. andre.g.senecal.civ@mail.mil.

ABSTRACT
An electrotextile with a biosensing focus composed of conductive polymer coated microfibers that contain functional attachment sites for biorecognition elements was developed. Experiments were conducted to select a compound with a pendant functional group for inclusion in the polymer, a fiber platform, and polymerization solvent. The effects of dopant inclusion and post-polymerization wash steps were also analyzed. Finally, the successful attachment of avidin, which was then used to capture biotin, to the electrotextile was achieved. The initial results show a nonwoven fiber matrix can be successfully coated in a conductive, functionalized polymer while still maintaining surface area and fiber durability. A polypropylene fiber platform with a conductive polypyrrole coating using iron (III) chloride as an oxidant, water as a solvent, and 5-sulfosalicylic acid as a dopant exhibited the best coating consistency, material durability, and lowest resistance. Biological attachment of avidin was achieved on the fibers through the inclusion of a carboxyl functional group via 3-thiopheneacetic acid in the monomer. The immobilized avidin was then successfully used to capture biotin. This was confirmed through the use of fluorescent quantum dots and confocal microscopy. A preliminary electrochemical experiment using avidin for biotin detection was conducted. This technology will be extremely useful in the formation of electrotextiles for use in biosensor systems.

No MeSH data available.


Average response over a period of 12 min (after a 3 min equilibrium time) for the electrochemical detection with varying biotin solution concentrations of 0.5 µM (green, triangle), 5 µM (purple, x), 50 µM (blue, diamond), and 500 µM (orange, circle). The system response increases as the concentration of biotin increases.
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biosensors-02-00465-f007: Average response over a period of 12 min (after a 3 min equilibrium time) for the electrochemical detection with varying biotin solution concentrations of 0.5 µM (green, triangle), 5 µM (purple, x), 50 µM (blue, diamond), and 500 µM (orange, circle). The system response increases as the concentration of biotin increases.

Mentions: Preliminary experiments were conducted taking multiple measurements using the fiber membranes as electrodes to determine if a biological recognition signal can be observed. Triplicate measurements were taken using the conductive fiber membrane electrodes to establish the resistance values for a control sample (0.1 M PB) and biotin solutions at concentrations of 0.5, 5, 50, and 500 µM. A time of 3 min was determined to be necessary to reach system equilibrium. The responses for each concentration at each time point were averaged and the value after the initial 3 min equilibrium time can be seen in Figure 7.


Synthesis of a functionalized polypyrrole coated electrotextile for use in biosensors.

McGraw SK, Alocilja E, Senecal A, Senecal K - Biosensors (Basel) (2012)

Average response over a period of 12 min (after a 3 min equilibrium time) for the electrochemical detection with varying biotin solution concentrations of 0.5 µM (green, triangle), 5 µM (purple, x), 50 µM (blue, diamond), and 500 µM (orange, circle). The system response increases as the concentration of biotin increases.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263555&req=5

biosensors-02-00465-f007: Average response over a period of 12 min (after a 3 min equilibrium time) for the electrochemical detection with varying biotin solution concentrations of 0.5 µM (green, triangle), 5 µM (purple, x), 50 µM (blue, diamond), and 500 µM (orange, circle). The system response increases as the concentration of biotin increases.
Mentions: Preliminary experiments were conducted taking multiple measurements using the fiber membranes as electrodes to determine if a biological recognition signal can be observed. Triplicate measurements were taken using the conductive fiber membrane electrodes to establish the resistance values for a control sample (0.1 M PB) and biotin solutions at concentrations of 0.5, 5, 50, and 500 µM. A time of 3 min was determined to be necessary to reach system equilibrium. The responses for each concentration at each time point were averaged and the value after the initial 3 min equilibrium time can be seen in Figure 7.

Bottom Line: The effects of dopant inclusion and post-polymerization wash steps were also analyzed.The initial results show a nonwoven fiber matrix can be successfully coated in a conductive, functionalized polymer while still maintaining surface area and fiber durability.The immobilized avidin was then successfully used to capture biotin.

View Article: PubMed Central - PubMed

Affiliation: Biosystems and Agricultural Engineering, Michigan State University, 524 S. Shaw Lane, 115 Farrall Hall, East Lansing, MI 48824, USA. andre.g.senecal.civ@mail.mil.

ABSTRACT
An electrotextile with a biosensing focus composed of conductive polymer coated microfibers that contain functional attachment sites for biorecognition elements was developed. Experiments were conducted to select a compound with a pendant functional group for inclusion in the polymer, a fiber platform, and polymerization solvent. The effects of dopant inclusion and post-polymerization wash steps were also analyzed. Finally, the successful attachment of avidin, which was then used to capture biotin, to the electrotextile was achieved. The initial results show a nonwoven fiber matrix can be successfully coated in a conductive, functionalized polymer while still maintaining surface area and fiber durability. A polypropylene fiber platform with a conductive polypyrrole coating using iron (III) chloride as an oxidant, water as a solvent, and 5-sulfosalicylic acid as a dopant exhibited the best coating consistency, material durability, and lowest resistance. Biological attachment of avidin was achieved on the fibers through the inclusion of a carboxyl functional group via 3-thiopheneacetic acid in the monomer. The immobilized avidin was then successfully used to capture biotin. This was confirmed through the use of fluorescent quantum dots and confocal microscopy. A preliminary electrochemical experiment using avidin for biotin detection was conducted. This technology will be extremely useful in the formation of electrotextiles for use in biosensor systems.

No MeSH data available.