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Synthesis of a functionalized polypyrrole coated electrotextile for use in biosensors.

McGraw SK, Alocilja E, Senecal A, Senecal K - Biosensors (Basel) (2012)

Bottom Line: The effects of dopant inclusion and post-polymerization wash steps were also analyzed.The initial results show a nonwoven fiber matrix can be successfully coated in a conductive, functionalized polymer while still maintaining surface area and fiber durability.The immobilized avidin was then successfully used to capture biotin.

View Article: PubMed Central - PubMed

Affiliation: Biosystems and Agricultural Engineering, Michigan State University, 524 S. Shaw Lane, 115 Farrall Hall, East Lansing, MI 48824, USA. andre.g.senecal.civ@mail.mil.

ABSTRACT
An electrotextile with a biosensing focus composed of conductive polymer coated microfibers that contain functional attachment sites for biorecognition elements was developed. Experiments were conducted to select a compound with a pendant functional group for inclusion in the polymer, a fiber platform, and polymerization solvent. The effects of dopant inclusion and post-polymerization wash steps were also analyzed. Finally, the successful attachment of avidin, which was then used to capture biotin, to the electrotextile was achieved. The initial results show a nonwoven fiber matrix can be successfully coated in a conductive, functionalized polymer while still maintaining surface area and fiber durability. A polypropylene fiber platform with a conductive polypyrrole coating using iron (III) chloride as an oxidant, water as a solvent, and 5-sulfosalicylic acid as a dopant exhibited the best coating consistency, material durability, and lowest resistance. Biological attachment of avidin was achieved on the fibers through the inclusion of a carboxyl functional group via 3-thiopheneacetic acid in the monomer. The immobilized avidin was then successfully used to capture biotin. This was confirmed through the use of fluorescent quantum dots and confocal microscopy. A preliminary electrochemical experiment using avidin for biotin detection was conducted. This technology will be extremely useful in the formation of electrotextiles for use in biosensor systems.

No MeSH data available.


False colored confocal images of fibers with biotinylated quantum dot attachment. (A) Fiber reflectance. (B) Bound biotinylated quantum dots. (C) Composite image. All at 4,000× with lasers at 405 nm.
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biosensors-02-00465-f006: False colored confocal images of fibers with biotinylated quantum dot attachment. (A) Fiber reflectance. (B) Bound biotinylated quantum dots. (C) Composite image. All at 4,000× with lasers at 405 nm.

Mentions: Generating a conductive polymer coating onto the fiber membranes has two purposes. The first is to make the fibers capable of conducting an electrical signal through the fibrous platform and the second is to provide attachment sites for biorecognition elements on these fibrous surfaces. Confocal microscopy was used to determine if FITC labeled avidin was covalently bound to the polymer coating. Qdot labeled biotin was then used to indicate if the surface bound avidin had maintained its capture ability. Figure 5 and Figure 6 confirm the presence of functional groups for bio-recognition attachment in the polymer. Figure 5 shows a coated fiber that has FITC-labeled avidin attached to it. Figure 6 shows the same fiber sample, after the addition of Qdot labeled biotin. The avidin has bound to the polymer coating and then conjugated with the biotin. This indicates that a biorecognition element, avidin, can be successfully bound to the polymer coating using covalent attachment chemistry and can be used to perform capture.


Synthesis of a functionalized polypyrrole coated electrotextile for use in biosensors.

McGraw SK, Alocilja E, Senecal A, Senecal K - Biosensors (Basel) (2012)

False colored confocal images of fibers with biotinylated quantum dot attachment. (A) Fiber reflectance. (B) Bound biotinylated quantum dots. (C) Composite image. All at 4,000× with lasers at 405 nm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263555&req=5

biosensors-02-00465-f006: False colored confocal images of fibers with biotinylated quantum dot attachment. (A) Fiber reflectance. (B) Bound biotinylated quantum dots. (C) Composite image. All at 4,000× with lasers at 405 nm.
Mentions: Generating a conductive polymer coating onto the fiber membranes has two purposes. The first is to make the fibers capable of conducting an electrical signal through the fibrous platform and the second is to provide attachment sites for biorecognition elements on these fibrous surfaces. Confocal microscopy was used to determine if FITC labeled avidin was covalently bound to the polymer coating. Qdot labeled biotin was then used to indicate if the surface bound avidin had maintained its capture ability. Figure 5 and Figure 6 confirm the presence of functional groups for bio-recognition attachment in the polymer. Figure 5 shows a coated fiber that has FITC-labeled avidin attached to it. Figure 6 shows the same fiber sample, after the addition of Qdot labeled biotin. The avidin has bound to the polymer coating and then conjugated with the biotin. This indicates that a biorecognition element, avidin, can be successfully bound to the polymer coating using covalent attachment chemistry and can be used to perform capture.

Bottom Line: The effects of dopant inclusion and post-polymerization wash steps were also analyzed.The initial results show a nonwoven fiber matrix can be successfully coated in a conductive, functionalized polymer while still maintaining surface area and fiber durability.The immobilized avidin was then successfully used to capture biotin.

View Article: PubMed Central - PubMed

Affiliation: Biosystems and Agricultural Engineering, Michigan State University, 524 S. Shaw Lane, 115 Farrall Hall, East Lansing, MI 48824, USA. andre.g.senecal.civ@mail.mil.

ABSTRACT
An electrotextile with a biosensing focus composed of conductive polymer coated microfibers that contain functional attachment sites for biorecognition elements was developed. Experiments were conducted to select a compound with a pendant functional group for inclusion in the polymer, a fiber platform, and polymerization solvent. The effects of dopant inclusion and post-polymerization wash steps were also analyzed. Finally, the successful attachment of avidin, which was then used to capture biotin, to the electrotextile was achieved. The initial results show a nonwoven fiber matrix can be successfully coated in a conductive, functionalized polymer while still maintaining surface area and fiber durability. A polypropylene fiber platform with a conductive polypyrrole coating using iron (III) chloride as an oxidant, water as a solvent, and 5-sulfosalicylic acid as a dopant exhibited the best coating consistency, material durability, and lowest resistance. Biological attachment of avidin was achieved on the fibers through the inclusion of a carboxyl functional group via 3-thiopheneacetic acid in the monomer. The immobilized avidin was then successfully used to capture biotin. This was confirmed through the use of fluorescent quantum dots and confocal microscopy. A preliminary electrochemical experiment using avidin for biotin detection was conducted. This technology will be extremely useful in the formation of electrotextiles for use in biosensor systems.

No MeSH data available.