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Synthesis of a functionalized polypyrrole coated electrotextile for use in biosensors.

McGraw SK, Alocilja E, Senecal A, Senecal K - Biosensors (Basel) (2012)

Bottom Line: The effects of dopant inclusion and post-polymerization wash steps were also analyzed.The initial results show a nonwoven fiber matrix can be successfully coated in a conductive, functionalized polymer while still maintaining surface area and fiber durability.The immobilized avidin was then successfully used to capture biotin.

View Article: PubMed Central - PubMed

Affiliation: Biosystems and Agricultural Engineering, Michigan State University, 524 S. Shaw Lane, 115 Farrall Hall, East Lansing, MI 48824, USA. andre.g.senecal.civ@mail.mil.

ABSTRACT
An electrotextile with a biosensing focus composed of conductive polymer coated microfibers that contain functional attachment sites for biorecognition elements was developed. Experiments were conducted to select a compound with a pendant functional group for inclusion in the polymer, a fiber platform, and polymerization solvent. The effects of dopant inclusion and post-polymerization wash steps were also analyzed. Finally, the successful attachment of avidin, which was then used to capture biotin, to the electrotextile was achieved. The initial results show a nonwoven fiber matrix can be successfully coated in a conductive, functionalized polymer while still maintaining surface area and fiber durability. A polypropylene fiber platform with a conductive polypyrrole coating using iron (III) chloride as an oxidant, water as a solvent, and 5-sulfosalicylic acid as a dopant exhibited the best coating consistency, material durability, and lowest resistance. Biological attachment of avidin was achieved on the fibers through the inclusion of a carboxyl functional group via 3-thiopheneacetic acid in the monomer. The immobilized avidin was then successfully used to capture biotin. This was confirmed through the use of fluorescent quantum dots and confocal microscopy. A preliminary electrochemical experiment using avidin for biotin detection was conducted. This technology will be extremely useful in the formation of electrotextiles for use in biosensor systems.

No MeSH data available.


SEM images of nylon 6 fibers coated in doped polypyrrole with 3 post-polymerization treatments. (A) No rinse. (B) DI water wash. (C) DI water wash and sonication. All at 500×.
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biosensors-02-00465-f003: SEM images of nylon 6 fibers coated in doped polypyrrole with 3 post-polymerization treatments. (A) No rinse. (B) DI water wash. (C) DI water wash and sonication. All at 500×.

Mentions: SEM analysis, shown in Figure 3, shows heavy, clustered polymer coatings along the fibers from each sample, with the sample that was not washed appearing to have a slightly heavier surface coating. The addition of a rinse step and sonication did not result in a significant loss of polymer coating from the fiber discs, however those samples did show better porosity between the individual fibers in the SEM images. The lack of a change in resistance and SEM images indicated that the polymer had bound to the nylon microfiber lattice. The larger clusters of polymer, where the polymer was attached to itself as opposed to the fiber surface, had weaker bonds, was removable and did not significantly change the resistance. Washing of the fibers post-polymerization was added to the protocol to allow for the removal of weakly bound excess, resulting in better fiber porosity and ensuring that the biorecognition elements would have access to the lower layers of the fiber mat.


Synthesis of a functionalized polypyrrole coated electrotextile for use in biosensors.

McGraw SK, Alocilja E, Senecal A, Senecal K - Biosensors (Basel) (2012)

SEM images of nylon 6 fibers coated in doped polypyrrole with 3 post-polymerization treatments. (A) No rinse. (B) DI water wash. (C) DI water wash and sonication. All at 500×.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263555&req=5

biosensors-02-00465-f003: SEM images of nylon 6 fibers coated in doped polypyrrole with 3 post-polymerization treatments. (A) No rinse. (B) DI water wash. (C) DI water wash and sonication. All at 500×.
Mentions: SEM analysis, shown in Figure 3, shows heavy, clustered polymer coatings along the fibers from each sample, with the sample that was not washed appearing to have a slightly heavier surface coating. The addition of a rinse step and sonication did not result in a significant loss of polymer coating from the fiber discs, however those samples did show better porosity between the individual fibers in the SEM images. The lack of a change in resistance and SEM images indicated that the polymer had bound to the nylon microfiber lattice. The larger clusters of polymer, where the polymer was attached to itself as opposed to the fiber surface, had weaker bonds, was removable and did not significantly change the resistance. Washing of the fibers post-polymerization was added to the protocol to allow for the removal of weakly bound excess, resulting in better fiber porosity and ensuring that the biorecognition elements would have access to the lower layers of the fiber mat.

Bottom Line: The effects of dopant inclusion and post-polymerization wash steps were also analyzed.The initial results show a nonwoven fiber matrix can be successfully coated in a conductive, functionalized polymer while still maintaining surface area and fiber durability.The immobilized avidin was then successfully used to capture biotin.

View Article: PubMed Central - PubMed

Affiliation: Biosystems and Agricultural Engineering, Michigan State University, 524 S. Shaw Lane, 115 Farrall Hall, East Lansing, MI 48824, USA. andre.g.senecal.civ@mail.mil.

ABSTRACT
An electrotextile with a biosensing focus composed of conductive polymer coated microfibers that contain functional attachment sites for biorecognition elements was developed. Experiments were conducted to select a compound with a pendant functional group for inclusion in the polymer, a fiber platform, and polymerization solvent. The effects of dopant inclusion and post-polymerization wash steps were also analyzed. Finally, the successful attachment of avidin, which was then used to capture biotin, to the electrotextile was achieved. The initial results show a nonwoven fiber matrix can be successfully coated in a conductive, functionalized polymer while still maintaining surface area and fiber durability. A polypropylene fiber platform with a conductive polypyrrole coating using iron (III) chloride as an oxidant, water as a solvent, and 5-sulfosalicylic acid as a dopant exhibited the best coating consistency, material durability, and lowest resistance. Biological attachment of avidin was achieved on the fibers through the inclusion of a carboxyl functional group via 3-thiopheneacetic acid in the monomer. The immobilized avidin was then successfully used to capture biotin. This was confirmed through the use of fluorescent quantum dots and confocal microscopy. A preliminary electrochemical experiment using avidin for biotin detection was conducted. This technology will be extremely useful in the formation of electrotextiles for use in biosensor systems.

No MeSH data available.