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Effect of toxic components on microbial fuel cell-polarization curves and estimation of the type of toxic inhibition.

Stein NE, Hamelers HV, van Straten G, Keesman KJ - Biosensors (Basel) (2012)

Bottom Line: In this study, polarization curves were made under non-toxic conditions and under toxic conditions after the addition of various concentrations of nickel, bentazon, sodiumdodecyl sulfate and potassium ferricyanide.For each of the toxic components, the value of the kinetic inhibition constant Ki was also estimated from the experimental data.The value of Ki indicates the sensitivity of the sensor for a specific component and thus can be used for the selection of the biosensor for a toxic component.

View Article: PubMed Central - PubMed

Affiliation: Wetsus, Centre of Excellence for Sustainable Water Technology, P.O. Box 1113, 8900CC Leeuwarden, The Netherlands. nienke.stein@tno.nl.

ABSTRACT
Polarization curves are of paramount importance for the detection of toxic components in microbial fuel cell (MFC) based biosensors. In this study, polarization curves were made under non-toxic conditions and under toxic conditions after the addition of various concentrations of nickel, bentazon, sodiumdodecyl sulfate and potassium ferricyanide. The experimental polarization curves show that toxic components have an effect on the electrochemically active bacteria in the cell. (Extended) Butler Volmer Monod (BVM) models were used to describe the polarization curves of the MFC under nontoxic and toxic conditions. It was possible to properly fit the (extended) BVM models using linear regression techniques to the polarization curves and to distinguish between different types of kinetic inhibitions. For each of the toxic components, the value of the kinetic inhibition constant Ki was also estimated from the experimental data. The value of Ki indicates the sensitivity of the sensor for a specific component and thus can be used for the selection of the biosensor for a toxic component.

No MeSH data available.


Related in: MedlinePlus

Effect of chemical components on the current (a) nickel. (b) sodium dodecyl sulfate (SDS). (c) bentazon. (d) potassium ferricyanide.
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biosensors-02-00255-f001: Effect of chemical components on the current (a) nickel. (b) sodium dodecyl sulfate (SDS). (c) bentazon. (d) potassium ferricyanide.

Mentions: As a first toxic component, nickel was dosed at three concentrations: 10, 20 and 30 mg/L. Data are shown in Figure 1(a). Comparing the polarization curve when nickel is present with the curve under non-toxic conditions, the curve shifts down when nickel is present in the sensor. The higher the concentration, the larger the shift. Hence, there seems to be a dose-response relationship of current change to nickel concentration. This was already found from experiments in the MFC-based biosensor at constant anode potential [5], but it is thus also found from the polarization curves.


Effect of toxic components on microbial fuel cell-polarization curves and estimation of the type of toxic inhibition.

Stein NE, Hamelers HV, van Straten G, Keesman KJ - Biosensors (Basel) (2012)

Effect of chemical components on the current (a) nickel. (b) sodium dodecyl sulfate (SDS). (c) bentazon. (d) potassium ferricyanide.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263553&req=5

biosensors-02-00255-f001: Effect of chemical components on the current (a) nickel. (b) sodium dodecyl sulfate (SDS). (c) bentazon. (d) potassium ferricyanide.
Mentions: As a first toxic component, nickel was dosed at three concentrations: 10, 20 and 30 mg/L. Data are shown in Figure 1(a). Comparing the polarization curve when nickel is present with the curve under non-toxic conditions, the curve shifts down when nickel is present in the sensor. The higher the concentration, the larger the shift. Hence, there seems to be a dose-response relationship of current change to nickel concentration. This was already found from experiments in the MFC-based biosensor at constant anode potential [5], but it is thus also found from the polarization curves.

Bottom Line: In this study, polarization curves were made under non-toxic conditions and under toxic conditions after the addition of various concentrations of nickel, bentazon, sodiumdodecyl sulfate and potassium ferricyanide.For each of the toxic components, the value of the kinetic inhibition constant Ki was also estimated from the experimental data.The value of Ki indicates the sensitivity of the sensor for a specific component and thus can be used for the selection of the biosensor for a toxic component.

View Article: PubMed Central - PubMed

Affiliation: Wetsus, Centre of Excellence for Sustainable Water Technology, P.O. Box 1113, 8900CC Leeuwarden, The Netherlands. nienke.stein@tno.nl.

ABSTRACT
Polarization curves are of paramount importance for the detection of toxic components in microbial fuel cell (MFC) based biosensors. In this study, polarization curves were made under non-toxic conditions and under toxic conditions after the addition of various concentrations of nickel, bentazon, sodiumdodecyl sulfate and potassium ferricyanide. The experimental polarization curves show that toxic components have an effect on the electrochemically active bacteria in the cell. (Extended) Butler Volmer Monod (BVM) models were used to describe the polarization curves of the MFC under nontoxic and toxic conditions. It was possible to properly fit the (extended) BVM models using linear regression techniques to the polarization curves and to distinguish between different types of kinetic inhibitions. For each of the toxic components, the value of the kinetic inhibition constant Ki was also estimated from the experimental data. The value of Ki indicates the sensitivity of the sensor for a specific component and thus can be used for the selection of the biosensor for a toxic component.

No MeSH data available.


Related in: MedlinePlus