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Thiol- and biotin-labeled probes for oligonucleotide quartz crystal microbalance biosensors of microalga alexandrium minutum.

Lazerges M, Perrot H, Rabehagasoa N, Compère C - Biosensors (Basel) (2012)

Bottom Line: Two quartz crystal microbalance oligonucleotide biosensors of a toxic microalga gene sequence (Alexandrium Minutum) have been designed.The frequency of the set up is stable to within a few hertz, corresponding to the nanogram scale, for three hour experiments.DNA recognition by the two biosensors is efficient and selective.

View Article: PubMed Central - PubMed

Affiliation: Centre National de la Recherche Scientifique (CNRS), Unité Propre de Recherche (UPR) 15, Laboratoire Interfaces et Systèmes Electrochimiques, 4 place Jussieu, Paris 75252, France. mathieu.lazerges@parisdescartes.fr.

ABSTRACT
Two quartz crystal microbalance oligonucleotide biosensors of a toxic microalga gene sequence (Alexandrium Minutum) have been designed. Grafting on a gold surface of 20-base thiol- or biotin-labeled probe, and selective hybridization with the complementary 20-base target, have been monitored in situ with a 27 MHz quartz crystal microbalance under controlled hydrodynamic conditions. The frequency of the set up is stable to within a few hertz, corresponding to the nanogram scale, for three hour experiments. DNA recognition by the two biosensors is efficient and selective. Hybridization kinetic curves indicate that the biosensor designed with the thiol-labeled probe is more sensitive, and that the biosensor designed with the biotin-labeled probe has a shorter time response and a higher hybridization efficiency.

No MeSH data available.


Microbalance frequency changes during avidin grafting, biotin-labeled probe (P3) grafting and selective complementary target (T2) recognition.
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biosensors-02-00245-f005: Microbalance frequency changes during avidin grafting, biotin-labeled probe (P3) grafting and selective complementary target (T2) recognition.

Mentions: The frequency was not recorded during formation of the self-assembled dithiopropionic acid and EDC ester activation, due to the low molecular weight of these compounds, being too feeble for detection with a 27 MHz quartz crystal microbalance sensitivity. Frequency changes during avidin grafting, biotin-labeled probe (P3) grafting and complementary target (T2) recognition, are presented in Figure 5.


Thiol- and biotin-labeled probes for oligonucleotide quartz crystal microbalance biosensors of microalga alexandrium minutum.

Lazerges M, Perrot H, Rabehagasoa N, Compère C - Biosensors (Basel) (2012)

Microbalance frequency changes during avidin grafting, biotin-labeled probe (P3) grafting and selective complementary target (T2) recognition.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263548&req=5

biosensors-02-00245-f005: Microbalance frequency changes during avidin grafting, biotin-labeled probe (P3) grafting and selective complementary target (T2) recognition.
Mentions: The frequency was not recorded during formation of the self-assembled dithiopropionic acid and EDC ester activation, due to the low molecular weight of these compounds, being too feeble for detection with a 27 MHz quartz crystal microbalance sensitivity. Frequency changes during avidin grafting, biotin-labeled probe (P3) grafting and complementary target (T2) recognition, are presented in Figure 5.

Bottom Line: Two quartz crystal microbalance oligonucleotide biosensors of a toxic microalga gene sequence (Alexandrium Minutum) have been designed.The frequency of the set up is stable to within a few hertz, corresponding to the nanogram scale, for three hour experiments.DNA recognition by the two biosensors is efficient and selective.

View Article: PubMed Central - PubMed

Affiliation: Centre National de la Recherche Scientifique (CNRS), Unité Propre de Recherche (UPR) 15, Laboratoire Interfaces et Systèmes Electrochimiques, 4 place Jussieu, Paris 75252, France. mathieu.lazerges@parisdescartes.fr.

ABSTRACT
Two quartz crystal microbalance oligonucleotide biosensors of a toxic microalga gene sequence (Alexandrium Minutum) have been designed. Grafting on a gold surface of 20-base thiol- or biotin-labeled probe, and selective hybridization with the complementary 20-base target, have been monitored in situ with a 27 MHz quartz crystal microbalance under controlled hydrodynamic conditions. The frequency of the set up is stable to within a few hertz, corresponding to the nanogram scale, for three hour experiments. DNA recognition by the two biosensors is efficient and selective. Hybridization kinetic curves indicate that the biosensor designed with the thiol-labeled probe is more sensitive, and that the biosensor designed with the biotin-labeled probe has a shorter time response and a higher hybridization efficiency.

No MeSH data available.