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Thiol- and biotin-labeled probes for oligonucleotide quartz crystal microbalance biosensors of microalga alexandrium minutum.

Lazerges M, Perrot H, Rabehagasoa N, Compère C - Biosensors (Basel) (2012)

Bottom Line: Two quartz crystal microbalance oligonucleotide biosensors of a toxic microalga gene sequence (Alexandrium Minutum) have been designed.The frequency of the set up is stable to within a few hertz, corresponding to the nanogram scale, for three hour experiments.DNA recognition by the two biosensors is efficient and selective.

View Article: PubMed Central - PubMed

Affiliation: Centre National de la Recherche Scientifique (CNRS), Unité Propre de Recherche (UPR) 15, Laboratoire Interfaces et Systèmes Electrochimiques, 4 place Jussieu, Paris 75252, France. mathieu.lazerges@parisdescartes.fr.

ABSTRACT
Two quartz crystal microbalance oligonucleotide biosensors of a toxic microalga gene sequence (Alexandrium Minutum) have been designed. Grafting on a gold surface of 20-base thiol- or biotin-labeled probe, and selective hybridization with the complementary 20-base target, have been monitored in situ with a 27 MHz quartz crystal microbalance under controlled hydrodynamic conditions. The frequency of the set up is stable to within a few hertz, corresponding to the nanogram scale, for three hour experiments. DNA recognition by the two biosensors is efficient and selective. Hybridization kinetic curves indicate that the biosensor designed with the thiol-labeled probe is more sensitive, and that the biosensor designed with the biotin-labeled probe has a shorter time response and a higher hybridization efficiency.

No MeSH data available.


Hybridization colorimetric test on nylon membranes of Alexandrium Minutum complementary probes P1 (non labeled), P2 (thiol-labeled), P3 (biotin-labeled) and non complementary DNA R (random) with T1 complementary target (phosphatase-labeled).
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biosensors-02-00245-f001: Hybridization colorimetric test on nylon membranes of Alexandrium Minutum complementary probes P1 (non labeled), P2 (thiol-labeled), P3 (biotin-labeled) and non complementary DNA R (random) with T1 complementary target (phosphatase-labeled).

Mentions: Hybridization experiments are first performed on nylon membranes in order to check hybridization of thiol- and biotin-labeled probes. These tests are performed with random probe (R), and Alexandrium Minutum probes P1, P2 (thiol-labeled) and P3 (biotin-labeled). Hybridization nylon membrane colorimetric tests with phosphatase-labeled complementary target (T1) are presented in Figure 1.


Thiol- and biotin-labeled probes for oligonucleotide quartz crystal microbalance biosensors of microalga alexandrium minutum.

Lazerges M, Perrot H, Rabehagasoa N, Compère C - Biosensors (Basel) (2012)

Hybridization colorimetric test on nylon membranes of Alexandrium Minutum complementary probes P1 (non labeled), P2 (thiol-labeled), P3 (biotin-labeled) and non complementary DNA R (random) with T1 complementary target (phosphatase-labeled).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263548&req=5

biosensors-02-00245-f001: Hybridization colorimetric test on nylon membranes of Alexandrium Minutum complementary probes P1 (non labeled), P2 (thiol-labeled), P3 (biotin-labeled) and non complementary DNA R (random) with T1 complementary target (phosphatase-labeled).
Mentions: Hybridization experiments are first performed on nylon membranes in order to check hybridization of thiol- and biotin-labeled probes. These tests are performed with random probe (R), and Alexandrium Minutum probes P1, P2 (thiol-labeled) and P3 (biotin-labeled). Hybridization nylon membrane colorimetric tests with phosphatase-labeled complementary target (T1) are presented in Figure 1.

Bottom Line: Two quartz crystal microbalance oligonucleotide biosensors of a toxic microalga gene sequence (Alexandrium Minutum) have been designed.The frequency of the set up is stable to within a few hertz, corresponding to the nanogram scale, for three hour experiments.DNA recognition by the two biosensors is efficient and selective.

View Article: PubMed Central - PubMed

Affiliation: Centre National de la Recherche Scientifique (CNRS), Unité Propre de Recherche (UPR) 15, Laboratoire Interfaces et Systèmes Electrochimiques, 4 place Jussieu, Paris 75252, France. mathieu.lazerges@parisdescartes.fr.

ABSTRACT
Two quartz crystal microbalance oligonucleotide biosensors of a toxic microalga gene sequence (Alexandrium Minutum) have been designed. Grafting on a gold surface of 20-base thiol- or biotin-labeled probe, and selective hybridization with the complementary 20-base target, have been monitored in situ with a 27 MHz quartz crystal microbalance under controlled hydrodynamic conditions. The frequency of the set up is stable to within a few hertz, corresponding to the nanogram scale, for three hour experiments. DNA recognition by the two biosensors is efficient and selective. Hybridization kinetic curves indicate that the biosensor designed with the thiol-labeled probe is more sensitive, and that the biosensor designed with the biotin-labeled probe has a shorter time response and a higher hybridization efficiency.

No MeSH data available.