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Electrochemical biosensor for rapid and sensitive detection of magnetically extracted bacterial pathogens.

Setterington EB, Alocilja EC - Biosensors (Basel) (2012)

Bottom Line: Cyclic voltammetry is combined with immunomagnetic separation in a rapid method requiring approximately 1 h for presumptive positive/negative results.The presence of target cells significantly inhibits current flow between the electrically active c/sNPs and SPCE.This method has the potential to be adapted for a wide variety of target organisms and sample matrices, and to become a fully portable system for routine monitoring or emergency detection of bacterial pathogens.

View Article: PubMed Central - PubMed

Affiliation: Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USA. ebs@msu.edu.

ABSTRACT
Biological defense and security applications demand rapid, sensitive detection of bacterial pathogens. This work presents a novel qualitative electrochemical detection technique which is applied to two representative bacterial pathogens, Bacillus cereus (as a surrogate for B. anthracis) and Escherichia coli O157:H7, resulting in detection limits of 40 CFU/mL and 6 CFU/mL, respectively, from pure culture. Cyclic voltammetry is combined with immunomagnetic separation in a rapid method requiring approximately 1 h for presumptive positive/negative results. An immunofunctionalized magnetic/polyaniline core/shell nano-particle (c/sNP) is employed to extract target cells from the sample solution and magnetically position them on a screen-printed carbon electrode (SPCE) sensor. The presence of target cells significantly inhibits current flow between the electrically active c/sNPs and SPCE. This method has the potential to be adapted for a wide variety of target organisms and sample matrices, and to become a fully portable system for routine monitoring or emergency detection of bacterial pathogens.

No MeSH data available.


Related in: MedlinePlus

Schematic of the SPCE sensor: (a) top view; (b) cross-sectional view.
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biosensors-02-00015-f001: Schematic of the SPCE sensor: (a) top view; (b) cross-sectional view.

Mentions: Cyclic voltammetric measurements were performed with a 263A potentiostat/galvanostat (Princeton Applied Research, MA, USA) connected to a personal computer. Data collection and analysis was controlled with the PowerSuite electrochemical software operating system (Princeton Applied Research, MA, USA). Screen-printed carbon electrode (SPCE) sensors (Gwent Inc., UK) are shown in Figure 1. The sensor is composed of two electrodes: the inner carbon/graphite working electrode, having a diameter of 4 mm, and the outer silver/silver chloride counter/reference electrode. A 200 μL capacity sample well is defined by an insulating foam. Every SPCE sensor was rinsed with sterile DI water and allowed to dry before test solution was applied. Sensors were disposed of after a single use.


Electrochemical biosensor for rapid and sensitive detection of magnetically extracted bacterial pathogens.

Setterington EB, Alocilja EC - Biosensors (Basel) (2012)

Schematic of the SPCE sensor: (a) top view; (b) cross-sectional view.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263547&req=5

biosensors-02-00015-f001: Schematic of the SPCE sensor: (a) top view; (b) cross-sectional view.
Mentions: Cyclic voltammetric measurements were performed with a 263A potentiostat/galvanostat (Princeton Applied Research, MA, USA) connected to a personal computer. Data collection and analysis was controlled with the PowerSuite electrochemical software operating system (Princeton Applied Research, MA, USA). Screen-printed carbon electrode (SPCE) sensors (Gwent Inc., UK) are shown in Figure 1. The sensor is composed of two electrodes: the inner carbon/graphite working electrode, having a diameter of 4 mm, and the outer silver/silver chloride counter/reference electrode. A 200 μL capacity sample well is defined by an insulating foam. Every SPCE sensor was rinsed with sterile DI water and allowed to dry before test solution was applied. Sensors were disposed of after a single use.

Bottom Line: Cyclic voltammetry is combined with immunomagnetic separation in a rapid method requiring approximately 1 h for presumptive positive/negative results.The presence of target cells significantly inhibits current flow between the electrically active c/sNPs and SPCE.This method has the potential to be adapted for a wide variety of target organisms and sample matrices, and to become a fully portable system for routine monitoring or emergency detection of bacterial pathogens.

View Article: PubMed Central - PubMed

Affiliation: Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USA. ebs@msu.edu.

ABSTRACT
Biological defense and security applications demand rapid, sensitive detection of bacterial pathogens. This work presents a novel qualitative electrochemical detection technique which is applied to two representative bacterial pathogens, Bacillus cereus (as a surrogate for B. anthracis) and Escherichia coli O157:H7, resulting in detection limits of 40 CFU/mL and 6 CFU/mL, respectively, from pure culture. Cyclic voltammetry is combined with immunomagnetic separation in a rapid method requiring approximately 1 h for presumptive positive/negative results. An immunofunctionalized magnetic/polyaniline core/shell nano-particle (c/sNP) is employed to extract target cells from the sample solution and magnetically position them on a screen-printed carbon electrode (SPCE) sensor. The presence of target cells significantly inhibits current flow between the electrically active c/sNPs and SPCE. This method has the potential to be adapted for a wide variety of target organisms and sample matrices, and to become a fully portable system for routine monitoring or emergency detection of bacterial pathogens.

No MeSH data available.


Related in: MedlinePlus