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Enzyme-gelatin electrochemical biosensors: scaling down.

De Wael K, De Belder S, Pilehvar S, Van Steenberge G, Herrebout W, Heering HA - Biosensors (Basel) (2012)

Bottom Line: By spincoating, highly uniform sub micrometer layers of biocompatible matrices can be constructed.A full electrochemical study and characterization of the modified surfaces has been carried out.It was clear that in the case of catalase, gluteraldehyde addition was needed to prevent leaking of the catalase from the gelatin matrix.

View Article: PubMed Central - PubMed

Affiliation: Environmental Analysis, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium. Karolien.DeWael@ua.ac.be.

ABSTRACT
In this article we investigate the possibility of scaling down enzyme-gelatin modified electrodes by spin coating the enzyme-gelatin layer. Special attention is given to the electrochemical behavior of the selected enzymes inside the gelatin matrix. A glassy carbon electrode was used as a substrate to immobilize, in the first instance, horse heart cytochrome c (HHC) in a gelatin matrix. Both a drop dried and a spin coated layer was prepared. On scaling down, a transition from diffusion controlled reactions towards adsorption controlled reactions is observed. Compared to a drop dried electrode, a spin coated electrode showed a more stable electrochemical behavior. Next to HHC, we also incorporated catalase in a spin coated gelatin matrix immobilized on a glassy carbon electrode. By spincoating, highly uniform sub micrometer layers of biocompatible matrices can be constructed. A full electrochemical study and characterization of the modified surfaces has been carried out. It was clear that in the case of catalase, gluteraldehyde addition was needed to prevent leaking of the catalase from the gelatin matrix.

No MeSH data available.


(a,b) Scanning Electron Microscope (SEM) image of a Gel/C electrode; (c,d) SEM image of a Cat/Gel/C electrode; (e,f) SEM image of a Cat/Gel/C electrode which was soaked in a buffer solution for 24 h.
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biosensors-02-00101-f007: (a,b) Scanning Electron Microscope (SEM) image of a Gel/C electrode; (c,d) SEM image of a Cat/Gel/C electrode; (e,f) SEM image of a Cat/Gel/C electrode which was soaked in a buffer solution for 24 h.

Mentions: Figure 7 represents the SEM images of the surface of a glassy carbon electrode with pure gelatin (a,b) and catalase entrapped gelatin films (c,d). A significant difference between the surface structure of pure gelatin and Cat/gelatin is observed. In addition, the gelatin film structure seems to be very uniform and regularly flat. In contrast with pure gelatin, as can be seen in Figure 7(c,d), catalase immobilized gelatin layers have an asymmetric and uneven structure pattern which illustrates successful catalase immobilization in a gelatin network. Moreover, this structure change suggests that the interaction between gelatin film and catalase occurs and influences the morphology of the films [28,29].


Enzyme-gelatin electrochemical biosensors: scaling down.

De Wael K, De Belder S, Pilehvar S, Van Steenberge G, Herrebout W, Heering HA - Biosensors (Basel) (2012)

(a,b) Scanning Electron Microscope (SEM) image of a Gel/C electrode; (c,d) SEM image of a Cat/Gel/C electrode; (e,f) SEM image of a Cat/Gel/C electrode which was soaked in a buffer solution for 24 h.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263544&req=5

biosensors-02-00101-f007: (a,b) Scanning Electron Microscope (SEM) image of a Gel/C electrode; (c,d) SEM image of a Cat/Gel/C electrode; (e,f) SEM image of a Cat/Gel/C electrode which was soaked in a buffer solution for 24 h.
Mentions: Figure 7 represents the SEM images of the surface of a glassy carbon electrode with pure gelatin (a,b) and catalase entrapped gelatin films (c,d). A significant difference between the surface structure of pure gelatin and Cat/gelatin is observed. In addition, the gelatin film structure seems to be very uniform and regularly flat. In contrast with pure gelatin, as can be seen in Figure 7(c,d), catalase immobilized gelatin layers have an asymmetric and uneven structure pattern which illustrates successful catalase immobilization in a gelatin network. Moreover, this structure change suggests that the interaction between gelatin film and catalase occurs and influences the morphology of the films [28,29].

Bottom Line: By spincoating, highly uniform sub micrometer layers of biocompatible matrices can be constructed.A full electrochemical study and characterization of the modified surfaces has been carried out.It was clear that in the case of catalase, gluteraldehyde addition was needed to prevent leaking of the catalase from the gelatin matrix.

View Article: PubMed Central - PubMed

Affiliation: Environmental Analysis, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium. Karolien.DeWael@ua.ac.be.

ABSTRACT
In this article we investigate the possibility of scaling down enzyme-gelatin modified electrodes by spin coating the enzyme-gelatin layer. Special attention is given to the electrochemical behavior of the selected enzymes inside the gelatin matrix. A glassy carbon electrode was used as a substrate to immobilize, in the first instance, horse heart cytochrome c (HHC) in a gelatin matrix. Both a drop dried and a spin coated layer was prepared. On scaling down, a transition from diffusion controlled reactions towards adsorption controlled reactions is observed. Compared to a drop dried electrode, a spin coated electrode showed a more stable electrochemical behavior. Next to HHC, we also incorporated catalase in a spin coated gelatin matrix immobilized on a glassy carbon electrode. By spincoating, highly uniform sub micrometer layers of biocompatible matrices can be constructed. A full electrochemical study and characterization of the modified surfaces has been carried out. It was clear that in the case of catalase, gluteraldehyde addition was needed to prevent leaking of the catalase from the gelatin matrix.

No MeSH data available.