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Enzyme-gelatin electrochemical biosensors: scaling down.

De Wael K, De Belder S, Pilehvar S, Van Steenberge G, Herrebout W, Heering HA - Biosensors (Basel) (2012)

Bottom Line: By spincoating, highly uniform sub micrometer layers of biocompatible matrices can be constructed.A full electrochemical study and characterization of the modified surfaces has been carried out.It was clear that in the case of catalase, gluteraldehyde addition was needed to prevent leaking of the catalase from the gelatin matrix.

View Article: PubMed Central - PubMed

Affiliation: Environmental Analysis, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium. Karolien.DeWael@ua.ac.be.

ABSTRACT
In this article we investigate the possibility of scaling down enzyme-gelatin modified electrodes by spin coating the enzyme-gelatin layer. Special attention is given to the electrochemical behavior of the selected enzymes inside the gelatin matrix. A glassy carbon electrode was used as a substrate to immobilize, in the first instance, horse heart cytochrome c (HHC) in a gelatin matrix. Both a drop dried and a spin coated layer was prepared. On scaling down, a transition from diffusion controlled reactions towards adsorption controlled reactions is observed. Compared to a drop dried electrode, a spin coated electrode showed a more stable electrochemical behavior. Next to HHC, we also incorporated catalase in a spin coated gelatin matrix immobilized on a glassy carbon electrode. By spincoating, highly uniform sub micrometer layers of biocompatible matrices can be constructed. A full electrochemical study and characterization of the modified surfaces has been carried out. It was clear that in the case of catalase, gluteraldehyde addition was needed to prevent leaking of the catalase from the gelatin matrix.

No MeSH data available.


Optical profile measurement showing a gelatin layer thickness of 7 µm. The gelatin was removed locally using an Excimer laser.
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biosensors-02-00101-f006: Optical profile measurement showing a gelatin layer thickness of 7 µm. The gelatin was removed locally using an Excimer laser.

Mentions: To measure the thickness of a SC gelatin layer, a hole was made in the gelatin film with an Excimer laser and the thickness of the film was measured at the edge using a Wyko NT3300 non-contact optical profiler (Figure 6). Following this approach, one avoids the estimation of the index of refraction of the gelatin film needed when measuring through the film. Results show that the average thickness of the Cat-gelatin film is 7 µm.


Enzyme-gelatin electrochemical biosensors: scaling down.

De Wael K, De Belder S, Pilehvar S, Van Steenberge G, Herrebout W, Heering HA - Biosensors (Basel) (2012)

Optical profile measurement showing a gelatin layer thickness of 7 µm. The gelatin was removed locally using an Excimer laser.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263544&req=5

biosensors-02-00101-f006: Optical profile measurement showing a gelatin layer thickness of 7 µm. The gelatin was removed locally using an Excimer laser.
Mentions: To measure the thickness of a SC gelatin layer, a hole was made in the gelatin film with an Excimer laser and the thickness of the film was measured at the edge using a Wyko NT3300 non-contact optical profiler (Figure 6). Following this approach, one avoids the estimation of the index of refraction of the gelatin film needed when measuring through the film. Results show that the average thickness of the Cat-gelatin film is 7 µm.

Bottom Line: By spincoating, highly uniform sub micrometer layers of biocompatible matrices can be constructed.A full electrochemical study and characterization of the modified surfaces has been carried out.It was clear that in the case of catalase, gluteraldehyde addition was needed to prevent leaking of the catalase from the gelatin matrix.

View Article: PubMed Central - PubMed

Affiliation: Environmental Analysis, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium. Karolien.DeWael@ua.ac.be.

ABSTRACT
In this article we investigate the possibility of scaling down enzyme-gelatin modified electrodes by spin coating the enzyme-gelatin layer. Special attention is given to the electrochemical behavior of the selected enzymes inside the gelatin matrix. A glassy carbon electrode was used as a substrate to immobilize, in the first instance, horse heart cytochrome c (HHC) in a gelatin matrix. Both a drop dried and a spin coated layer was prepared. On scaling down, a transition from diffusion controlled reactions towards adsorption controlled reactions is observed. Compared to a drop dried electrode, a spin coated electrode showed a more stable electrochemical behavior. Next to HHC, we also incorporated catalase in a spin coated gelatin matrix immobilized on a glassy carbon electrode. By spincoating, highly uniform sub micrometer layers of biocompatible matrices can be constructed. A full electrochemical study and characterization of the modified surfaces has been carried out. It was clear that in the case of catalase, gluteraldehyde addition was needed to prevent leaking of the catalase from the gelatin matrix.

No MeSH data available.