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Enzyme-gelatin electrochemical biosensors: scaling down.

De Wael K, De Belder S, Pilehvar S, Van Steenberge G, Herrebout W, Heering HA - Biosensors (Basel) (2012)

Bottom Line: By spincoating, highly uniform sub micrometer layers of biocompatible matrices can be constructed.A full electrochemical study and characterization of the modified surfaces has been carried out.It was clear that in the case of catalase, gluteraldehyde addition was needed to prevent leaking of the catalase from the gelatin matrix.

View Article: PubMed Central - PubMed

Affiliation: Environmental Analysis, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium. Karolien.DeWael@ua.ac.be.

ABSTRACT
In this article we investigate the possibility of scaling down enzyme-gelatin modified electrodes by spin coating the enzyme-gelatin layer. Special attention is given to the electrochemical behavior of the selected enzymes inside the gelatin matrix. A glassy carbon electrode was used as a substrate to immobilize, in the first instance, horse heart cytochrome c (HHC) in a gelatin matrix. Both a drop dried and a spin coated layer was prepared. On scaling down, a transition from diffusion controlled reactions towards adsorption controlled reactions is observed. Compared to a drop dried electrode, a spin coated electrode showed a more stable electrochemical behavior. Next to HHC, we also incorporated catalase in a spin coated gelatin matrix immobilized on a glassy carbon electrode. By spincoating, highly uniform sub micrometer layers of biocompatible matrices can be constructed. A full electrochemical study and characterization of the modified surfaces has been carried out. It was clear that in the case of catalase, gluteraldehyde addition was needed to prevent leaking of the catalase from the gelatin matrix.

No MeSH data available.


Related in: MedlinePlus

The current potential behavior of a spin coated GelB/C (1–2) and a spin coated Cat/GelB/C (3) electrode in the absence (1) and presence of 10.3 mmol∙L−1 H2O2 (2–3) in a 10 mmol∙L−1 HEPES pH 7 buffer solution with a scan rate of 50 mV∙s−1. Curve 4 is the current potential behavior obtained after curve 3 and polishing of the electrode.
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biosensors-02-00101-f005: The current potential behavior of a spin coated GelB/C (1–2) and a spin coated Cat/GelB/C (3) electrode in the absence (1) and presence of 10.3 mmol∙L−1 H2O2 (2–3) in a 10 mmol∙L−1 HEPES pH 7 buffer solution with a scan rate of 50 mV∙s−1. Curve 4 is the current potential behavior obtained after curve 3 and polishing of the electrode.

Mentions: The electrochemical behavior of a SC gelatin layer on top of a glassy carbon electrode in a HEPES pH 7 buffer solution is shown as curve 1 in Figure 5. No oxidation or reduction processes from gelatin were observed in this specific potential window. When 10.3 mmol∙L−1 H2O2 is added to the solution, curve 2 is obtained, representing the behavior of hydrogen peroxide towards a glassy carbon electrode.


Enzyme-gelatin electrochemical biosensors: scaling down.

De Wael K, De Belder S, Pilehvar S, Van Steenberge G, Herrebout W, Heering HA - Biosensors (Basel) (2012)

The current potential behavior of a spin coated GelB/C (1–2) and a spin coated Cat/GelB/C (3) electrode in the absence (1) and presence of 10.3 mmol∙L−1 H2O2 (2–3) in a 10 mmol∙L−1 HEPES pH 7 buffer solution with a scan rate of 50 mV∙s−1. Curve 4 is the current potential behavior obtained after curve 3 and polishing of the electrode.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263544&req=5

biosensors-02-00101-f005: The current potential behavior of a spin coated GelB/C (1–2) and a spin coated Cat/GelB/C (3) electrode in the absence (1) and presence of 10.3 mmol∙L−1 H2O2 (2–3) in a 10 mmol∙L−1 HEPES pH 7 buffer solution with a scan rate of 50 mV∙s−1. Curve 4 is the current potential behavior obtained after curve 3 and polishing of the electrode.
Mentions: The electrochemical behavior of a SC gelatin layer on top of a glassy carbon electrode in a HEPES pH 7 buffer solution is shown as curve 1 in Figure 5. No oxidation or reduction processes from gelatin were observed in this specific potential window. When 10.3 mmol∙L−1 H2O2 is added to the solution, curve 2 is obtained, representing the behavior of hydrogen peroxide towards a glassy carbon electrode.

Bottom Line: By spincoating, highly uniform sub micrometer layers of biocompatible matrices can be constructed.A full electrochemical study and characterization of the modified surfaces has been carried out.It was clear that in the case of catalase, gluteraldehyde addition was needed to prevent leaking of the catalase from the gelatin matrix.

View Article: PubMed Central - PubMed

Affiliation: Environmental Analysis, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium. Karolien.DeWael@ua.ac.be.

ABSTRACT
In this article we investigate the possibility of scaling down enzyme-gelatin modified electrodes by spin coating the enzyme-gelatin layer. Special attention is given to the electrochemical behavior of the selected enzymes inside the gelatin matrix. A glassy carbon electrode was used as a substrate to immobilize, in the first instance, horse heart cytochrome c (HHC) in a gelatin matrix. Both a drop dried and a spin coated layer was prepared. On scaling down, a transition from diffusion controlled reactions towards adsorption controlled reactions is observed. Compared to a drop dried electrode, a spin coated electrode showed a more stable electrochemical behavior. Next to HHC, we also incorporated catalase in a spin coated gelatin matrix immobilized on a glassy carbon electrode. By spincoating, highly uniform sub micrometer layers of biocompatible matrices can be constructed. A full electrochemical study and characterization of the modified surfaces has been carried out. It was clear that in the case of catalase, gluteraldehyde addition was needed to prevent leaking of the catalase from the gelatin matrix.

No MeSH data available.


Related in: MedlinePlus