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Enzyme-gelatin electrochemical biosensors: scaling down.

De Wael K, De Belder S, Pilehvar S, Van Steenberge G, Herrebout W, Heering HA - Biosensors (Basel) (2012)

Bottom Line: By spincoating, highly uniform sub micrometer layers of biocompatible matrices can be constructed.A full electrochemical study and characterization of the modified surfaces has been carried out.It was clear that in the case of catalase, gluteraldehyde addition was needed to prevent leaking of the catalase from the gelatin matrix.

View Article: PubMed Central - PubMed

Affiliation: Environmental Analysis, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium. Karolien.DeWael@ua.ac.be.

ABSTRACT
In this article we investigate the possibility of scaling down enzyme-gelatin modified electrodes by spin coating the enzyme-gelatin layer. Special attention is given to the electrochemical behavior of the selected enzymes inside the gelatin matrix. A glassy carbon electrode was used as a substrate to immobilize, in the first instance, horse heart cytochrome c (HHC) in a gelatin matrix. Both a drop dried and a spin coated layer was prepared. On scaling down, a transition from diffusion controlled reactions towards adsorption controlled reactions is observed. Compared to a drop dried electrode, a spin coated electrode showed a more stable electrochemical behavior. Next to HHC, we also incorporated catalase in a spin coated gelatin matrix immobilized on a glassy carbon electrode. By spincoating, highly uniform sub micrometer layers of biocompatible matrices can be constructed. A full electrochemical study and characterization of the modified surfaces has been carried out. It was clear that in the case of catalase, gluteraldehyde addition was needed to prevent leaking of the catalase from the gelatin matrix.

No MeSH data available.


ATR-IR spectra of a spin coated GelB/C (black, 1), Cat/GelB/C (green, 2) and HHC/GelB/C (red, 3) electrode after background correction.
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biosensors-02-00101-f004: ATR-IR spectra of a spin coated GelB/C (black, 1), Cat/GelB/C (green, 2) and HHC/GelB/C (red, 3) electrode after background correction.

Mentions: IR measurements were used to give further experimental evidence of the formation of a spin coated gelatin layer. The blank measurement (bare glassy carbon) was subtracted from all recorded IR spectra. Curves 1–3 in Figure 4 are the ATR-IR spectra of a spin coated GelB/GC (black, 1), Cat/GelB/GC (green, 2) and HHC/GelB/GC (red, 3) electrode respectively. In all cases, the characteristic bands for gelatin could be observed [16,18], indicating the presence of the latter on the electrode surfaces. No difference could be made between the gelatin films (1) on top of a glassy carbon electrode and the gelatin enzyme films (2–3). Thus, the presence of enzymes inside the spin coated gelatin film does not affect the spectra. In contrast, the IR spectra of a drop dried HHC/GelB/GC electrode showed distinct differences when compared to a drop dried GelB/GC electrode [16]. This phenomenon could be explained by the fact that for spin coated enzyme-gelatin layers a more homogeneous layer of gelatin (containing enzymes) is attained because of the more forced interaction between both compounds due to the spin coating procedure.


Enzyme-gelatin electrochemical biosensors: scaling down.

De Wael K, De Belder S, Pilehvar S, Van Steenberge G, Herrebout W, Heering HA - Biosensors (Basel) (2012)

ATR-IR spectra of a spin coated GelB/C (black, 1), Cat/GelB/C (green, 2) and HHC/GelB/C (red, 3) electrode after background correction.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263544&req=5

biosensors-02-00101-f004: ATR-IR spectra of a spin coated GelB/C (black, 1), Cat/GelB/C (green, 2) and HHC/GelB/C (red, 3) electrode after background correction.
Mentions: IR measurements were used to give further experimental evidence of the formation of a spin coated gelatin layer. The blank measurement (bare glassy carbon) was subtracted from all recorded IR spectra. Curves 1–3 in Figure 4 are the ATR-IR spectra of a spin coated GelB/GC (black, 1), Cat/GelB/GC (green, 2) and HHC/GelB/GC (red, 3) electrode respectively. In all cases, the characteristic bands for gelatin could be observed [16,18], indicating the presence of the latter on the electrode surfaces. No difference could be made between the gelatin films (1) on top of a glassy carbon electrode and the gelatin enzyme films (2–3). Thus, the presence of enzymes inside the spin coated gelatin film does not affect the spectra. In contrast, the IR spectra of a drop dried HHC/GelB/GC electrode showed distinct differences when compared to a drop dried GelB/GC electrode [16]. This phenomenon could be explained by the fact that for spin coated enzyme-gelatin layers a more homogeneous layer of gelatin (containing enzymes) is attained because of the more forced interaction between both compounds due to the spin coating procedure.

Bottom Line: By spincoating, highly uniform sub micrometer layers of biocompatible matrices can be constructed.A full electrochemical study and characterization of the modified surfaces has been carried out.It was clear that in the case of catalase, gluteraldehyde addition was needed to prevent leaking of the catalase from the gelatin matrix.

View Article: PubMed Central - PubMed

Affiliation: Environmental Analysis, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium. Karolien.DeWael@ua.ac.be.

ABSTRACT
In this article we investigate the possibility of scaling down enzyme-gelatin modified electrodes by spin coating the enzyme-gelatin layer. Special attention is given to the electrochemical behavior of the selected enzymes inside the gelatin matrix. A glassy carbon electrode was used as a substrate to immobilize, in the first instance, horse heart cytochrome c (HHC) in a gelatin matrix. Both a drop dried and a spin coated layer was prepared. On scaling down, a transition from diffusion controlled reactions towards adsorption controlled reactions is observed. Compared to a drop dried electrode, a spin coated electrode showed a more stable electrochemical behavior. Next to HHC, we also incorporated catalase in a spin coated gelatin matrix immobilized on a glassy carbon electrode. By spincoating, highly uniform sub micrometer layers of biocompatible matrices can be constructed. A full electrochemical study and characterization of the modified surfaces has been carried out. It was clear that in the case of catalase, gluteraldehyde addition was needed to prevent leaking of the catalase from the gelatin matrix.

No MeSH data available.