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Enzyme-gelatin electrochemical biosensors: scaling down.

De Wael K, De Belder S, Pilehvar S, Van Steenberge G, Herrebout W, Heering HA - Biosensors (Basel) (2012)

Bottom Line: By spincoating, highly uniform sub micrometer layers of biocompatible matrices can be constructed.A full electrochemical study and characterization of the modified surfaces has been carried out.It was clear that in the case of catalase, gluteraldehyde addition was needed to prevent leaking of the catalase from the gelatin matrix.

View Article: PubMed Central - PubMed

Affiliation: Environmental Analysis, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium. Karolien.DeWael@ua.ac.be.

ABSTRACT
In this article we investigate the possibility of scaling down enzyme-gelatin modified electrodes by spin coating the enzyme-gelatin layer. Special attention is given to the electrochemical behavior of the selected enzymes inside the gelatin matrix. A glassy carbon electrode was used as a substrate to immobilize, in the first instance, horse heart cytochrome c (HHC) in a gelatin matrix. Both a drop dried and a spin coated layer was prepared. On scaling down, a transition from diffusion controlled reactions towards adsorption controlled reactions is observed. Compared to a drop dried electrode, a spin coated electrode showed a more stable electrochemical behavior. Next to HHC, we also incorporated catalase in a spin coated gelatin matrix immobilized on a glassy carbon electrode. By spincoating, highly uniform sub micrometer layers of biocompatible matrices can be constructed. A full electrochemical study and characterization of the modified surfaces has been carried out. It was clear that in the case of catalase, gluteraldehyde addition was needed to prevent leaking of the catalase from the gelatin matrix.

No MeSH data available.


Logarithmic plot between the peak current Ip and the scan rate for a spin coated HHC/GelB/GC electrode.
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biosensors-02-00101-f002: Logarithmic plot between the peak current Ip and the scan rate for a spin coated HHC/GelB/GC electrode.

Mentions: When the HHC containing gelatin layer was immobilized onto the glassy carbon electrode by spin coating, we obtained a much thinner biocompatible layer on top of the electrode surface. Curve 3 is the current-potential behavior of a spin coated (SC) HHC/GelB/GC electrode. This peak shape of oxidation and reduction reactions indicates surface controlled reactions. So, by scaling down, the electrochemical behavior, diffusion controlled reactions evolve to adsorption controlled reactions. To prove this adsorption behavior, logI is plotted versus log(scan rate) (Figure 2). The slope of 1.1 indicates the adsorption nature of the process [38].


Enzyme-gelatin electrochemical biosensors: scaling down.

De Wael K, De Belder S, Pilehvar S, Van Steenberge G, Herrebout W, Heering HA - Biosensors (Basel) (2012)

Logarithmic plot between the peak current Ip and the scan rate for a spin coated HHC/GelB/GC electrode.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263544&req=5

biosensors-02-00101-f002: Logarithmic plot between the peak current Ip and the scan rate for a spin coated HHC/GelB/GC electrode.
Mentions: When the HHC containing gelatin layer was immobilized onto the glassy carbon electrode by spin coating, we obtained a much thinner biocompatible layer on top of the electrode surface. Curve 3 is the current-potential behavior of a spin coated (SC) HHC/GelB/GC electrode. This peak shape of oxidation and reduction reactions indicates surface controlled reactions. So, by scaling down, the electrochemical behavior, diffusion controlled reactions evolve to adsorption controlled reactions. To prove this adsorption behavior, logI is plotted versus log(scan rate) (Figure 2). The slope of 1.1 indicates the adsorption nature of the process [38].

Bottom Line: By spincoating, highly uniform sub micrometer layers of biocompatible matrices can be constructed.A full electrochemical study and characterization of the modified surfaces has been carried out.It was clear that in the case of catalase, gluteraldehyde addition was needed to prevent leaking of the catalase from the gelatin matrix.

View Article: PubMed Central - PubMed

Affiliation: Environmental Analysis, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium. Karolien.DeWael@ua.ac.be.

ABSTRACT
In this article we investigate the possibility of scaling down enzyme-gelatin modified electrodes by spin coating the enzyme-gelatin layer. Special attention is given to the electrochemical behavior of the selected enzymes inside the gelatin matrix. A glassy carbon electrode was used as a substrate to immobilize, in the first instance, horse heart cytochrome c (HHC) in a gelatin matrix. Both a drop dried and a spin coated layer was prepared. On scaling down, a transition from diffusion controlled reactions towards adsorption controlled reactions is observed. Compared to a drop dried electrode, a spin coated electrode showed a more stable electrochemical behavior. Next to HHC, we also incorporated catalase in a spin coated gelatin matrix immobilized on a glassy carbon electrode. By spincoating, highly uniform sub micrometer layers of biocompatible matrices can be constructed. A full electrochemical study and characterization of the modified surfaces has been carried out. It was clear that in the case of catalase, gluteraldehyde addition was needed to prevent leaking of the catalase from the gelatin matrix.

No MeSH data available.