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Poly(lactic acid)/Carbon Nanotube Fibers as Novel Platforms for Glucose Biosensors.

Oliveira JE, Mattoso LH, Medeiros ES, Zucolotto V - Biosensors (Basel) (2012)

Bottom Line: We investigated the effect of carbon nanotube concentration and the time deposition of fibers on the sensors properties, viz., sensitivity and limit of detection.These results demonstrate that the solution blow spun nanocomposite fibers have great potential for application as amperometric biosensors due to their high surface to volume ratio, high porosity and permeability of the substrate.The latter features may significantly enhance the field of glucose biosensors.

View Article: PubMed Central - PubMed

Affiliation: PPGCEM, Departamento de Engenharia de Materiais (DEMA), Universidade Federal de São Carlos (UFSCAR), Rodovia Washington Luis, km 235, Monjolinho, 13.565-905, São Carlos, SP, Brazil. julianoufmg@yahoo.com.br.

ABSTRACT
The focus of this paper is the development and investigation of properties of new nanostructured architecture for biosensors applications. Highly porous nanocomposite fibers were developed for use as active materials in biosensors. The nanocomposites comprised poly(lactic acid)(PLA)/multi-walled carbon nanotube (MWCNT) fibers obtained via solution-blow spinning onto indium tin oxide (ITO) electrodes. The electrocatalytic properties of nanocomposite-modified ITO electrodes were investigated toward hydrogen peroxide (H2O2) detection. We investigated the effect of carbon nanotube concentration and the time deposition of fibers on the sensors properties, viz., sensitivity and limit of detection. Cyclic voltammetry experiments revealed that the nanocomposite-modified electrodes displayed enhanced activity in the electrochemical reduction of H2O2, which offers a number of attractive features to be explored in development of an amperometric biosensor. Glucose oxidase (GOD) was further immobilized by drop coating on an optimized ITO electrode covered by poly(lactic acid)/carbon nanotube nanofibrous mats. The optimum biosensor response was linear up to 800 mM of glucose with a sensitivity of 358 nA·mM-1 and a Michaelis-Menten constant (KM) of 4.3 mM. These results demonstrate that the solution blow spun nanocomposite fibers have great potential for application as amperometric biosensors due to their high surface to volume ratio, high porosity and permeability of the substrate. The latter features may significantly enhance the field of glucose biosensors.

No MeSH data available.


(a) Effect of time deposition of poly(lactic acid)(PLA) fibers on cyclic voltammetric response at a scan rate of 50 mV·s−1. (I) 0 min; (II) 1 min; (III) 3 min and (IV) 15 min deposition time; (b) Peak current versus scan rate for mats deposited for 1 min on modified indium tin oxide (ITO) electrodes.
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biosensors-02-00070-f001: (a) Effect of time deposition of poly(lactic acid)(PLA) fibers on cyclic voltammetric response at a scan rate of 50 mV·s−1. (I) 0 min; (II) 1 min; (III) 3 min and (IV) 15 min deposition time; (b) Peak current versus scan rate for mats deposited for 1 min on modified indium tin oxide (ITO) electrodes.

Mentions: Cyclic voltammetry was performed in a 5 mM [Fe(CN)6]3−/4− probe solution containing H2SO4 (0.1 M). As shown in Figure 1(a), a pair of well-defined redox peaks of [Fe(CN)6]3−/4− probe was observed for the ITO bare electrode (Figure 1(a-I)), as expected. These peaks were still well-defined for electrodes containing fibers deposited for 1 min (Figure 1(a-II)), indicating the porosity and permeability of the spun mat on the ITO surface. A remarkable decrease of peak current was obtained at 3 min (Figure 1(a-III)) and 15 min (Figure 1(a-IV)) of deposition, suggesting that the bulkier fibers blocked the electron exchange between the redox probe and electrode surface.


Poly(lactic acid)/Carbon Nanotube Fibers as Novel Platforms for Glucose Biosensors.

Oliveira JE, Mattoso LH, Medeiros ES, Zucolotto V - Biosensors (Basel) (2012)

(a) Effect of time deposition of poly(lactic acid)(PLA) fibers on cyclic voltammetric response at a scan rate of 50 mV·s−1. (I) 0 min; (II) 1 min; (III) 3 min and (IV) 15 min deposition time; (b) Peak current versus scan rate for mats deposited for 1 min on modified indium tin oxide (ITO) electrodes.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263541&req=5

biosensors-02-00070-f001: (a) Effect of time deposition of poly(lactic acid)(PLA) fibers on cyclic voltammetric response at a scan rate of 50 mV·s−1. (I) 0 min; (II) 1 min; (III) 3 min and (IV) 15 min deposition time; (b) Peak current versus scan rate for mats deposited for 1 min on modified indium tin oxide (ITO) electrodes.
Mentions: Cyclic voltammetry was performed in a 5 mM [Fe(CN)6]3−/4− probe solution containing H2SO4 (0.1 M). As shown in Figure 1(a), a pair of well-defined redox peaks of [Fe(CN)6]3−/4− probe was observed for the ITO bare electrode (Figure 1(a-I)), as expected. These peaks were still well-defined for electrodes containing fibers deposited for 1 min (Figure 1(a-II)), indicating the porosity and permeability of the spun mat on the ITO surface. A remarkable decrease of peak current was obtained at 3 min (Figure 1(a-III)) and 15 min (Figure 1(a-IV)) of deposition, suggesting that the bulkier fibers blocked the electron exchange between the redox probe and electrode surface.

Bottom Line: We investigated the effect of carbon nanotube concentration and the time deposition of fibers on the sensors properties, viz., sensitivity and limit of detection.These results demonstrate that the solution blow spun nanocomposite fibers have great potential for application as amperometric biosensors due to their high surface to volume ratio, high porosity and permeability of the substrate.The latter features may significantly enhance the field of glucose biosensors.

View Article: PubMed Central - PubMed

Affiliation: PPGCEM, Departamento de Engenharia de Materiais (DEMA), Universidade Federal de São Carlos (UFSCAR), Rodovia Washington Luis, km 235, Monjolinho, 13.565-905, São Carlos, SP, Brazil. julianoufmg@yahoo.com.br.

ABSTRACT
The focus of this paper is the development and investigation of properties of new nanostructured architecture for biosensors applications. Highly porous nanocomposite fibers were developed for use as active materials in biosensors. The nanocomposites comprised poly(lactic acid)(PLA)/multi-walled carbon nanotube (MWCNT) fibers obtained via solution-blow spinning onto indium tin oxide (ITO) electrodes. The electrocatalytic properties of nanocomposite-modified ITO electrodes were investigated toward hydrogen peroxide (H2O2) detection. We investigated the effect of carbon nanotube concentration and the time deposition of fibers on the sensors properties, viz., sensitivity and limit of detection. Cyclic voltammetry experiments revealed that the nanocomposite-modified electrodes displayed enhanced activity in the electrochemical reduction of H2O2, which offers a number of attractive features to be explored in development of an amperometric biosensor. Glucose oxidase (GOD) was further immobilized by drop coating on an optimized ITO electrode covered by poly(lactic acid)/carbon nanotube nanofibrous mats. The optimum biosensor response was linear up to 800 mM of glucose with a sensitivity of 358 nA·mM-1 and a Michaelis-Menten constant (KM) of 4.3 mM. These results demonstrate that the solution blow spun nanocomposite fibers have great potential for application as amperometric biosensors due to their high surface to volume ratio, high porosity and permeability of the substrate. The latter features may significantly enhance the field of glucose biosensors.

No MeSH data available.