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Fabrication of biocompatible, vibrational magnetoelastic materials for controlling cellular adhesion.

Holmes HR, Tan EL, Ong KG, Rajachar RM - Biosensors (Basel) (2012)

Bottom Line: However, since ME materials are not inherently biocompatible, surface modifications are needed for their implementation in biological settings.In vitro cytotoxicity measurement and characterization of the vibrational behavior of the ME materials showed that Parylene-C coatings of 10 µm or greater could prevent hydrolytic degradation without sacrificing the vibrational behavior of the ME material.This work allows for long-term durability and functionality of ME materials in an aqueous and biological environment and makes the potential use of this technology in monitoring and modulating cellular behavior at the surface of implantable devices feasible.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, Michigan Technological University, Houghton, MI 49931, USA. hrholmes@mtu.edu.

ABSTRACT
This paper describes the functionalization of magnetoelastic (ME) materials with Parylene-C coating to improve the surface reactivity to cellular response. Previous study has demonstrated that vibrating ME materials were capable of modulating cellular adhesion when activated by an externally applied AC magnetic field. However, since ME materials are not inherently biocompatible, surface modifications are needed for their implementation in biological settings. Here, the long-term stability of the ME material in an aqueous and biological environment is achieved by chemical-vapor deposition of a conformal Parylene-C layer, and further functionalized by methods of oxygen plasma etching and protein adsorption. In vitro cytotoxicity measurement and characterization of the vibrational behavior of the ME materials showed that Parylene-C coatings of 10 µm or greater could prevent hydrolytic degradation without sacrificing the vibrational behavior of the ME material. This work allows for long-term durability and functionality of ME materials in an aqueous and biological environment and makes the potential use of this technology in monitoring and modulating cellular behavior at the surface of implantable devices feasible.

No MeSH data available.


Related in: MedlinePlus

Parylene-C maintains ME material integrity in vivo. Images of ME material before (a) and after (b) implantation show no observable traces of corrosion after 30 days of subcutaneous implantation in BALB/c mice.
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biosensors-02-00057-f008: Parylene-C maintains ME material integrity in vivo. Images of ME material before (a) and after (b) implantation show no observable traces of corrosion after 30 days of subcutaneous implantation in BALB/c mice.

Mentions: The application of 10 µm and 0.5 min plasma treated Parylene-C coating on ME material was shown to provide for material stability when subjected to a healthy mammalian host response. Qualitative assessment showed no signs of corrosion on explanted ME material after 30 days implantation (Figure 8). Furthermore, mice did not show any visible systemic effects to implants, exhibiting normal grooming and dietary behavior throughout the implantation period. This result demonstrated the feasibility and durability of plasma-treated and Parylene-C coated ME material within a dynamic biologic environment. Subsequent animal studies will be able to use this approach to explore in situ responses to local vibrational therapy where material stability inside a physiological environment is crucial to ensure long-term functionality of this technology.


Fabrication of biocompatible, vibrational magnetoelastic materials for controlling cellular adhesion.

Holmes HR, Tan EL, Ong KG, Rajachar RM - Biosensors (Basel) (2012)

Parylene-C maintains ME material integrity in vivo. Images of ME material before (a) and after (b) implantation show no observable traces of corrosion after 30 days of subcutaneous implantation in BALB/c mice.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263540&req=5

biosensors-02-00057-f008: Parylene-C maintains ME material integrity in vivo. Images of ME material before (a) and after (b) implantation show no observable traces of corrosion after 30 days of subcutaneous implantation in BALB/c mice.
Mentions: The application of 10 µm and 0.5 min plasma treated Parylene-C coating on ME material was shown to provide for material stability when subjected to a healthy mammalian host response. Qualitative assessment showed no signs of corrosion on explanted ME material after 30 days implantation (Figure 8). Furthermore, mice did not show any visible systemic effects to implants, exhibiting normal grooming and dietary behavior throughout the implantation period. This result demonstrated the feasibility and durability of plasma-treated and Parylene-C coated ME material within a dynamic biologic environment. Subsequent animal studies will be able to use this approach to explore in situ responses to local vibrational therapy where material stability inside a physiological environment is crucial to ensure long-term functionality of this technology.

Bottom Line: However, since ME materials are not inherently biocompatible, surface modifications are needed for their implementation in biological settings.In vitro cytotoxicity measurement and characterization of the vibrational behavior of the ME materials showed that Parylene-C coatings of 10 µm or greater could prevent hydrolytic degradation without sacrificing the vibrational behavior of the ME material.This work allows for long-term durability and functionality of ME materials in an aqueous and biological environment and makes the potential use of this technology in monitoring and modulating cellular behavior at the surface of implantable devices feasible.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, Michigan Technological University, Houghton, MI 49931, USA. hrholmes@mtu.edu.

ABSTRACT
This paper describes the functionalization of magnetoelastic (ME) materials with Parylene-C coating to improve the surface reactivity to cellular response. Previous study has demonstrated that vibrating ME materials were capable of modulating cellular adhesion when activated by an externally applied AC magnetic field. However, since ME materials are not inherently biocompatible, surface modifications are needed for their implementation in biological settings. Here, the long-term stability of the ME material in an aqueous and biological environment is achieved by chemical-vapor deposition of a conformal Parylene-C layer, and further functionalized by methods of oxygen plasma etching and protein adsorption. In vitro cytotoxicity measurement and characterization of the vibrational behavior of the ME materials showed that Parylene-C coatings of 10 µm or greater could prevent hydrolytic degradation without sacrificing the vibrational behavior of the ME material. This work allows for long-term durability and functionality of ME materials in an aqueous and biological environment and makes the potential use of this technology in monitoring and modulating cellular behavior at the surface of implantable devices feasible.

No MeSH data available.


Related in: MedlinePlus