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Fabrication of biocompatible, vibrational magnetoelastic materials for controlling cellular adhesion.

Holmes HR, Tan EL, Ong KG, Rajachar RM - Biosensors (Basel) (2012)

Bottom Line: However, since ME materials are not inherently biocompatible, surface modifications are needed for their implementation in biological settings.In vitro cytotoxicity measurement and characterization of the vibrational behavior of the ME materials showed that Parylene-C coatings of 10 µm or greater could prevent hydrolytic degradation without sacrificing the vibrational behavior of the ME material.This work allows for long-term durability and functionality of ME materials in an aqueous and biological environment and makes the potential use of this technology in monitoring and modulating cellular behavior at the surface of implantable devices feasible.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, Michigan Technological University, Houghton, MI 49931, USA. hrholmes@mtu.edu.

ABSTRACT
This paper describes the functionalization of magnetoelastic (ME) materials with Parylene-C coating to improve the surface reactivity to cellular response. Previous study has demonstrated that vibrating ME materials were capable of modulating cellular adhesion when activated by an externally applied AC magnetic field. However, since ME materials are not inherently biocompatible, surface modifications are needed for their implementation in biological settings. Here, the long-term stability of the ME material in an aqueous and biological environment is achieved by chemical-vapor deposition of a conformal Parylene-C layer, and further functionalized by methods of oxygen plasma etching and protein adsorption. In vitro cytotoxicity measurement and characterization of the vibrational behavior of the ME materials showed that Parylene-C coatings of 10 µm or greater could prevent hydrolytic degradation without sacrificing the vibrational behavior of the ME material. This work allows for long-term durability and functionality of ME materials in an aqueous and biological environment and makes the potential use of this technology in monitoring and modulating cellular behavior at the surface of implantable devices feasible.

No MeSH data available.


Related in: MedlinePlus

ME vibrations control cellular adhesion on functionalized (plasma-treated) Parylene-C surfaces. L929 fibroblasts cultured on Parylene-C coated ME materials plasma-treated for 0.5 mi (A–C) or adsorbed with vitronectin (D–F) showed significant reductions in cellular adhesion when subjected to ME vibrations. Cells cultured on Parylene-C adsorbed with fibronectin showed changes in cell morphology when subjected to ME vibrations, but significant detachment was not observed (G–I). Statistically significant differences (p<0.01) between groups are indicated (*).
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biosensors-02-00057-f007: ME vibrations control cellular adhesion on functionalized (plasma-treated) Parylene-C surfaces. L929 fibroblasts cultured on Parylene-C coated ME materials plasma-treated for 0.5 mi (A–C) or adsorbed with vitronectin (D–F) showed significant reductions in cellular adhesion when subjected to ME vibrations. Cells cultured on Parylene-C adsorbed with fibronectin showed changes in cell morphology when subjected to ME vibrations, but significant detachment was not observed (G–I). Statistically significant differences (p<0.01) between groups are indicated (*).

Mentions: The application of ME vibrations to fibroblasts cultured on functionalized Parylene-C coatings resulted in significant reductions (p < 0.01) in cell adhesion for samples adsorbed with vitronectin and plasma-treated for 0.5 min (Figure 7(A–F)). Qualitatively, noticeable changes in cell morphology (diminished cell spreading and increased roundness) were observed on samples absorbed with fibronectin, however there was no significant detachment (p < 0.0793) (Figure 7(G–I)). This outcome demonstrated the efficacy of working ME sensors in conjunction with a Parylene-C coating to modulate cell adhesion. The result also demonstrated the mechanical stability of the Parylene-C coating layer in a cell culture environment for 2 days (additional experiments showed the mechanical stability of the Parylene-C coating for a minimum of 6 months under culture conditions). Furthermore, this result showed that different functionalization techniques of Parylene-C coatings could be used to provide levels of control over the resulting cellular response to ME mechanical loading.


Fabrication of biocompatible, vibrational magnetoelastic materials for controlling cellular adhesion.

Holmes HR, Tan EL, Ong KG, Rajachar RM - Biosensors (Basel) (2012)

ME vibrations control cellular adhesion on functionalized (plasma-treated) Parylene-C surfaces. L929 fibroblasts cultured on Parylene-C coated ME materials plasma-treated for 0.5 mi (A–C) or adsorbed with vitronectin (D–F) showed significant reductions in cellular adhesion when subjected to ME vibrations. Cells cultured on Parylene-C adsorbed with fibronectin showed changes in cell morphology when subjected to ME vibrations, but significant detachment was not observed (G–I). Statistically significant differences (p<0.01) between groups are indicated (*).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263540&req=5

biosensors-02-00057-f007: ME vibrations control cellular adhesion on functionalized (plasma-treated) Parylene-C surfaces. L929 fibroblasts cultured on Parylene-C coated ME materials plasma-treated for 0.5 mi (A–C) or adsorbed with vitronectin (D–F) showed significant reductions in cellular adhesion when subjected to ME vibrations. Cells cultured on Parylene-C adsorbed with fibronectin showed changes in cell morphology when subjected to ME vibrations, but significant detachment was not observed (G–I). Statistically significant differences (p<0.01) between groups are indicated (*).
Mentions: The application of ME vibrations to fibroblasts cultured on functionalized Parylene-C coatings resulted in significant reductions (p < 0.01) in cell adhesion for samples adsorbed with vitronectin and plasma-treated for 0.5 min (Figure 7(A–F)). Qualitatively, noticeable changes in cell morphology (diminished cell spreading and increased roundness) were observed on samples absorbed with fibronectin, however there was no significant detachment (p < 0.0793) (Figure 7(G–I)). This outcome demonstrated the efficacy of working ME sensors in conjunction with a Parylene-C coating to modulate cell adhesion. The result also demonstrated the mechanical stability of the Parylene-C coating layer in a cell culture environment for 2 days (additional experiments showed the mechanical stability of the Parylene-C coating for a minimum of 6 months under culture conditions). Furthermore, this result showed that different functionalization techniques of Parylene-C coatings could be used to provide levels of control over the resulting cellular response to ME mechanical loading.

Bottom Line: However, since ME materials are not inherently biocompatible, surface modifications are needed for their implementation in biological settings.In vitro cytotoxicity measurement and characterization of the vibrational behavior of the ME materials showed that Parylene-C coatings of 10 µm or greater could prevent hydrolytic degradation without sacrificing the vibrational behavior of the ME material.This work allows for long-term durability and functionality of ME materials in an aqueous and biological environment and makes the potential use of this technology in monitoring and modulating cellular behavior at the surface of implantable devices feasible.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, Michigan Technological University, Houghton, MI 49931, USA. hrholmes@mtu.edu.

ABSTRACT
This paper describes the functionalization of magnetoelastic (ME) materials with Parylene-C coating to improve the surface reactivity to cellular response. Previous study has demonstrated that vibrating ME materials were capable of modulating cellular adhesion when activated by an externally applied AC magnetic field. However, since ME materials are not inherently biocompatible, surface modifications are needed for their implementation in biological settings. Here, the long-term stability of the ME material in an aqueous and biological environment is achieved by chemical-vapor deposition of a conformal Parylene-C layer, and further functionalized by methods of oxygen plasma etching and protein adsorption. In vitro cytotoxicity measurement and characterization of the vibrational behavior of the ME materials showed that Parylene-C coatings of 10 µm or greater could prevent hydrolytic degradation without sacrificing the vibrational behavior of the ME material. This work allows for long-term durability and functionality of ME materials in an aqueous and biological environment and makes the potential use of this technology in monitoring and modulating cellular behavior at the surface of implantable devices feasible.

No MeSH data available.


Related in: MedlinePlus