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Fabrication of biocompatible, vibrational magnetoelastic materials for controlling cellular adhesion.

Holmes HR, Tan EL, Ong KG, Rajachar RM - Biosensors (Basel) (2012)

Bottom Line: However, since ME materials are not inherently biocompatible, surface modifications are needed for their implementation in biological settings.In vitro cytotoxicity measurement and characterization of the vibrational behavior of the ME materials showed that Parylene-C coatings of 10 µm or greater could prevent hydrolytic degradation without sacrificing the vibrational behavior of the ME material.This work allows for long-term durability and functionality of ME materials in an aqueous and biological environment and makes the potential use of this technology in monitoring and modulating cellular behavior at the surface of implantable devices feasible.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, Michigan Technological University, Houghton, MI 49931, USA. hrholmes@mtu.edu.

ABSTRACT
This paper describes the functionalization of magnetoelastic (ME) materials with Parylene-C coating to improve the surface reactivity to cellular response. Previous study has demonstrated that vibrating ME materials were capable of modulating cellular adhesion when activated by an externally applied AC magnetic field. However, since ME materials are not inherently biocompatible, surface modifications are needed for their implementation in biological settings. Here, the long-term stability of the ME material in an aqueous and biological environment is achieved by chemical-vapor deposition of a conformal Parylene-C layer, and further functionalized by methods of oxygen plasma etching and protein adsorption. In vitro cytotoxicity measurement and characterization of the vibrational behavior of the ME materials showed that Parylene-C coatings of 10 µm or greater could prevent hydrolytic degradation without sacrificing the vibrational behavior of the ME material. This work allows for long-term durability and functionality of ME materials in an aqueous and biological environment and makes the potential use of this technology in monitoring and modulating cellular behavior at the surface of implantable devices feasible.

No MeSH data available.


Related in: MedlinePlus

The correlation of plasma treatment to ME material’s resonant frequency, and mass change. Resulting resonant frequency (A) was inversely proportional to mass changes (B) as a result of plasma etching with a strong linear trend.
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biosensors-02-00057-f006: The correlation of plasma treatment to ME material’s resonant frequency, and mass change. Resulting resonant frequency (A) was inversely proportional to mass changes (B) as a result of plasma etching with a strong linear trend.

Mentions: Using the ME material to monitor the effect of plasma etching on Parylene-C coated ME materials resulted in a trend of frequency increasing proportionally to etching time (R2 = 0.9401, Figure 6(A)). This trend is consistent with the previously described effect of plasma etching (the removal of benzene rings corresponds to an overall change in mass), which is also consistent with the previous finding where the increase of mass decreases the resonant frequency and vice versa. The relationship of etching time and mass change is shown in Figure 6(B). As demonstrated, oxygen plasma etching technique was capable of controlling the coating mass, thus the resonant frequency, by simply altering the etching time.


Fabrication of biocompatible, vibrational magnetoelastic materials for controlling cellular adhesion.

Holmes HR, Tan EL, Ong KG, Rajachar RM - Biosensors (Basel) (2012)

The correlation of plasma treatment to ME material’s resonant frequency, and mass change. Resulting resonant frequency (A) was inversely proportional to mass changes (B) as a result of plasma etching with a strong linear trend.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263540&req=5

biosensors-02-00057-f006: The correlation of plasma treatment to ME material’s resonant frequency, and mass change. Resulting resonant frequency (A) was inversely proportional to mass changes (B) as a result of plasma etching with a strong linear trend.
Mentions: Using the ME material to monitor the effect of plasma etching on Parylene-C coated ME materials resulted in a trend of frequency increasing proportionally to etching time (R2 = 0.9401, Figure 6(A)). This trend is consistent with the previously described effect of plasma etching (the removal of benzene rings corresponds to an overall change in mass), which is also consistent with the previous finding where the increase of mass decreases the resonant frequency and vice versa. The relationship of etching time and mass change is shown in Figure 6(B). As demonstrated, oxygen plasma etching technique was capable of controlling the coating mass, thus the resonant frequency, by simply altering the etching time.

Bottom Line: However, since ME materials are not inherently biocompatible, surface modifications are needed for their implementation in biological settings.In vitro cytotoxicity measurement and characterization of the vibrational behavior of the ME materials showed that Parylene-C coatings of 10 µm or greater could prevent hydrolytic degradation without sacrificing the vibrational behavior of the ME material.This work allows for long-term durability and functionality of ME materials in an aqueous and biological environment and makes the potential use of this technology in monitoring and modulating cellular behavior at the surface of implantable devices feasible.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, Michigan Technological University, Houghton, MI 49931, USA. hrholmes@mtu.edu.

ABSTRACT
This paper describes the functionalization of magnetoelastic (ME) materials with Parylene-C coating to improve the surface reactivity to cellular response. Previous study has demonstrated that vibrating ME materials were capable of modulating cellular adhesion when activated by an externally applied AC magnetic field. However, since ME materials are not inherently biocompatible, surface modifications are needed for their implementation in biological settings. Here, the long-term stability of the ME material in an aqueous and biological environment is achieved by chemical-vapor deposition of a conformal Parylene-C layer, and further functionalized by methods of oxygen plasma etching and protein adsorption. In vitro cytotoxicity measurement and characterization of the vibrational behavior of the ME materials showed that Parylene-C coatings of 10 µm or greater could prevent hydrolytic degradation without sacrificing the vibrational behavior of the ME material. This work allows for long-term durability and functionality of ME materials in an aqueous and biological environment and makes the potential use of this technology in monitoring and modulating cellular behavior at the surface of implantable devices feasible.

No MeSH data available.


Related in: MedlinePlus