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Fabrication of biocompatible, vibrational magnetoelastic materials for controlling cellular adhesion.

Holmes HR, Tan EL, Ong KG, Rajachar RM - Biosensors (Basel) (2012)

Bottom Line: However, since ME materials are not inherently biocompatible, surface modifications are needed for their implementation in biological settings.In vitro cytotoxicity measurement and characterization of the vibrational behavior of the ME materials showed that Parylene-C coatings of 10 µm or greater could prevent hydrolytic degradation without sacrificing the vibrational behavior of the ME material.This work allows for long-term durability and functionality of ME materials in an aqueous and biological environment and makes the potential use of this technology in monitoring and modulating cellular behavior at the surface of implantable devices feasible.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, Michigan Technological University, Houghton, MI 49931, USA. hrholmes@mtu.edu.

ABSTRACT
This paper describes the functionalization of magnetoelastic (ME) materials with Parylene-C coating to improve the surface reactivity to cellular response. Previous study has demonstrated that vibrating ME materials were capable of modulating cellular adhesion when activated by an externally applied AC magnetic field. However, since ME materials are not inherently biocompatible, surface modifications are needed for their implementation in biological settings. Here, the long-term stability of the ME material in an aqueous and biological environment is achieved by chemical-vapor deposition of a conformal Parylene-C layer, and further functionalized by methods of oxygen plasma etching and protein adsorption. In vitro cytotoxicity measurement and characterization of the vibrational behavior of the ME materials showed that Parylene-C coatings of 10 µm or greater could prevent hydrolytic degradation without sacrificing the vibrational behavior of the ME material. This work allows for long-term durability and functionality of ME materials in an aqueous and biological environment and makes the potential use of this technology in monitoring and modulating cellular behavior at the surface of implantable devices feasible.

No MeSH data available.


Related in: MedlinePlus

Surface properties characterization of plasma-treated and Parylene-C coated ME materials. (A) Surface roughness and (B) hydrophobicity analyses showed treatment methods involving plasma etching dramatically changed the surface character of Parylene-C while protein adsorption had relatively little effect on these specific surface properties. Statistically significant differences (p<0.01) between groups are indicated (*, †).
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biosensors-02-00057-f005: Surface properties characterization of plasma-treated and Parylene-C coated ME materials. (A) Surface roughness and (B) hydrophobicity analyses showed treatment methods involving plasma etching dramatically changed the surface character of Parylene-C while protein adsorption had relatively little effect on these specific surface properties. Statistically significant differences (p<0.01) between groups are indicated (*, †).

Mentions: Changes in cellular adhesion may be attributed to changes in surface properties (surface roughness and hydrophobicity) resulting from oxygen plasma treatment. As indicated in Figure 5, significantly (p < 0.01) different roughness for 0.5 and 3 min plasma-treated samples (Figure 5(A)), and significantly (p < 0.01) different hydrophobicity for all plasma-treated samples (Figure 5(B)) were evident when compared to untreated Parylene-C. However, it is interesting that similar levels of cellular adhesion occurred on samples etched for 0.5 and 1 min despite having significantly different surface roughness and hydrophobicity. Additionally, lower levels of cellular adhesion occurred on 3 and 5 min samples that possessed properties more similar to samples treated for 1 min. This result shows that plasma etching may also affect surface properties other than those directly investigated in this study, such as stiffness.


Fabrication of biocompatible, vibrational magnetoelastic materials for controlling cellular adhesion.

Holmes HR, Tan EL, Ong KG, Rajachar RM - Biosensors (Basel) (2012)

Surface properties characterization of plasma-treated and Parylene-C coated ME materials. (A) Surface roughness and (B) hydrophobicity analyses showed treatment methods involving plasma etching dramatically changed the surface character of Parylene-C while protein adsorption had relatively little effect on these specific surface properties. Statistically significant differences (p<0.01) between groups are indicated (*, †).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263540&req=5

biosensors-02-00057-f005: Surface properties characterization of plasma-treated and Parylene-C coated ME materials. (A) Surface roughness and (B) hydrophobicity analyses showed treatment methods involving plasma etching dramatically changed the surface character of Parylene-C while protein adsorption had relatively little effect on these specific surface properties. Statistically significant differences (p<0.01) between groups are indicated (*, †).
Mentions: Changes in cellular adhesion may be attributed to changes in surface properties (surface roughness and hydrophobicity) resulting from oxygen plasma treatment. As indicated in Figure 5, significantly (p < 0.01) different roughness for 0.5 and 3 min plasma-treated samples (Figure 5(A)), and significantly (p < 0.01) different hydrophobicity for all plasma-treated samples (Figure 5(B)) were evident when compared to untreated Parylene-C. However, it is interesting that similar levels of cellular adhesion occurred on samples etched for 0.5 and 1 min despite having significantly different surface roughness and hydrophobicity. Additionally, lower levels of cellular adhesion occurred on 3 and 5 min samples that possessed properties more similar to samples treated for 1 min. This result shows that plasma etching may also affect surface properties other than those directly investigated in this study, such as stiffness.

Bottom Line: However, since ME materials are not inherently biocompatible, surface modifications are needed for their implementation in biological settings.In vitro cytotoxicity measurement and characterization of the vibrational behavior of the ME materials showed that Parylene-C coatings of 10 µm or greater could prevent hydrolytic degradation without sacrificing the vibrational behavior of the ME material.This work allows for long-term durability and functionality of ME materials in an aqueous and biological environment and makes the potential use of this technology in monitoring and modulating cellular behavior at the surface of implantable devices feasible.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, Michigan Technological University, Houghton, MI 49931, USA. hrholmes@mtu.edu.

ABSTRACT
This paper describes the functionalization of magnetoelastic (ME) materials with Parylene-C coating to improve the surface reactivity to cellular response. Previous study has demonstrated that vibrating ME materials were capable of modulating cellular adhesion when activated by an externally applied AC magnetic field. However, since ME materials are not inherently biocompatible, surface modifications are needed for their implementation in biological settings. Here, the long-term stability of the ME material in an aqueous and biological environment is achieved by chemical-vapor deposition of a conformal Parylene-C layer, and further functionalized by methods of oxygen plasma etching and protein adsorption. In vitro cytotoxicity measurement and characterization of the vibrational behavior of the ME materials showed that Parylene-C coatings of 10 µm or greater could prevent hydrolytic degradation without sacrificing the vibrational behavior of the ME material. This work allows for long-term durability and functionality of ME materials in an aqueous and biological environment and makes the potential use of this technology in monitoring and modulating cellular behavior at the surface of implantable devices feasible.

No MeSH data available.


Related in: MedlinePlus