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Fabrication of biocompatible, vibrational magnetoelastic materials for controlling cellular adhesion.

Holmes HR, Tan EL, Ong KG, Rajachar RM - Biosensors (Basel) (2012)

Bottom Line: However, since ME materials are not inherently biocompatible, surface modifications are needed for their implementation in biological settings.In vitro cytotoxicity measurement and characterization of the vibrational behavior of the ME materials showed that Parylene-C coatings of 10 µm or greater could prevent hydrolytic degradation without sacrificing the vibrational behavior of the ME material.This work allows for long-term durability and functionality of ME materials in an aqueous and biological environment and makes the potential use of this technology in monitoring and modulating cellular behavior at the surface of implantable devices feasible.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, Michigan Technological University, Houghton, MI 49931, USA. hrholmes@mtu.edu.

ABSTRACT
This paper describes the functionalization of magnetoelastic (ME) materials with Parylene-C coating to improve the surface reactivity to cellular response. Previous study has demonstrated that vibrating ME materials were capable of modulating cellular adhesion when activated by an externally applied AC magnetic field. However, since ME materials are not inherently biocompatible, surface modifications are needed for their implementation in biological settings. Here, the long-term stability of the ME material in an aqueous and biological environment is achieved by chemical-vapor deposition of a conformal Parylene-C layer, and further functionalized by methods of oxygen plasma etching and protein adsorption. In vitro cytotoxicity measurement and characterization of the vibrational behavior of the ME materials showed that Parylene-C coatings of 10 µm or greater could prevent hydrolytic degradation without sacrificing the vibrational behavior of the ME material. This work allows for long-term durability and functionality of ME materials in an aqueous and biological environment and makes the potential use of this technology in monitoring and modulating cellular behavior at the surface of implantable devices feasible.

No MeSH data available.


Related in: MedlinePlus

A typical resonance of a ME material, where the resonance behavior is characterized by the resonant frequency (f0), resonant amplitude (A), and resonant quality (Q).
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biosensors-02-00057-f002: A typical resonance of a ME material, where the resonance behavior is characterized by the resonant frequency (f0), resonant amplitude (A), and resonant quality (Q).

Mentions: To investigate the effect of Parylene-C coating on the vibration of the ME material, the resonance behavior of the material was examined. As illustrated in Figure 2, useful characteristics of the ME resonance include: (i) the resonant frequency (f0), which is the frequency when the vibration of the material is at its highest; (ii) the resonant amplitude (S), which is the highest vibration amplitude; (iii) the resonant quality, which characterizes the spread of the resonant peak. The resonance of the ME material was captured using previously established approach [17]. Briefly, the ME materials were mechanically vibrated by exposing them to a selected frequency range of AC excitation field (160–165 kHz). In this study, ME materials coated with 5, 10, and 20 µm (n = 10 for each thickness) of Parylene-C were selected for investigation.


Fabrication of biocompatible, vibrational magnetoelastic materials for controlling cellular adhesion.

Holmes HR, Tan EL, Ong KG, Rajachar RM - Biosensors (Basel) (2012)

A typical resonance of a ME material, where the resonance behavior is characterized by the resonant frequency (f0), resonant amplitude (A), and resonant quality (Q).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263540&req=5

biosensors-02-00057-f002: A typical resonance of a ME material, where the resonance behavior is characterized by the resonant frequency (f0), resonant amplitude (A), and resonant quality (Q).
Mentions: To investigate the effect of Parylene-C coating on the vibration of the ME material, the resonance behavior of the material was examined. As illustrated in Figure 2, useful characteristics of the ME resonance include: (i) the resonant frequency (f0), which is the frequency when the vibration of the material is at its highest; (ii) the resonant amplitude (S), which is the highest vibration amplitude; (iii) the resonant quality, which characterizes the spread of the resonant peak. The resonance of the ME material was captured using previously established approach [17]. Briefly, the ME materials were mechanically vibrated by exposing them to a selected frequency range of AC excitation field (160–165 kHz). In this study, ME materials coated with 5, 10, and 20 µm (n = 10 for each thickness) of Parylene-C were selected for investigation.

Bottom Line: However, since ME materials are not inherently biocompatible, surface modifications are needed for their implementation in biological settings.In vitro cytotoxicity measurement and characterization of the vibrational behavior of the ME materials showed that Parylene-C coatings of 10 µm or greater could prevent hydrolytic degradation without sacrificing the vibrational behavior of the ME material.This work allows for long-term durability and functionality of ME materials in an aqueous and biological environment and makes the potential use of this technology in monitoring and modulating cellular behavior at the surface of implantable devices feasible.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, Michigan Technological University, Houghton, MI 49931, USA. hrholmes@mtu.edu.

ABSTRACT
This paper describes the functionalization of magnetoelastic (ME) materials with Parylene-C coating to improve the surface reactivity to cellular response. Previous study has demonstrated that vibrating ME materials were capable of modulating cellular adhesion when activated by an externally applied AC magnetic field. However, since ME materials are not inherently biocompatible, surface modifications are needed for their implementation in biological settings. Here, the long-term stability of the ME material in an aqueous and biological environment is achieved by chemical-vapor deposition of a conformal Parylene-C layer, and further functionalized by methods of oxygen plasma etching and protein adsorption. In vitro cytotoxicity measurement and characterization of the vibrational behavior of the ME materials showed that Parylene-C coatings of 10 µm or greater could prevent hydrolytic degradation without sacrificing the vibrational behavior of the ME material. This work allows for long-term durability and functionality of ME materials in an aqueous and biological environment and makes the potential use of this technology in monitoring and modulating cellular behavior at the surface of implantable devices feasible.

No MeSH data available.


Related in: MedlinePlus