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Fabrication of biocompatible, vibrational magnetoelastic materials for controlling cellular adhesion.

Holmes HR, Tan EL, Ong KG, Rajachar RM - Biosensors (Basel) (2012)

Bottom Line: However, since ME materials are not inherently biocompatible, surface modifications are needed for their implementation in biological settings.In vitro cytotoxicity measurement and characterization of the vibrational behavior of the ME materials showed that Parylene-C coatings of 10 µm or greater could prevent hydrolytic degradation without sacrificing the vibrational behavior of the ME material.This work allows for long-term durability and functionality of ME materials in an aqueous and biological environment and makes the potential use of this technology in monitoring and modulating cellular behavior at the surface of implantable devices feasible.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, Michigan Technological University, Houghton, MI 49931, USA. hrholmes@mtu.edu.

ABSTRACT
This paper describes the functionalization of magnetoelastic (ME) materials with Parylene-C coating to improve the surface reactivity to cellular response. Previous study has demonstrated that vibrating ME materials were capable of modulating cellular adhesion when activated by an externally applied AC magnetic field. However, since ME materials are not inherently biocompatible, surface modifications are needed for their implementation in biological settings. Here, the long-term stability of the ME material in an aqueous and biological environment is achieved by chemical-vapor deposition of a conformal Parylene-C layer, and further functionalized by methods of oxygen plasma etching and protein adsorption. In vitro cytotoxicity measurement and characterization of the vibrational behavior of the ME materials showed that Parylene-C coatings of 10 µm or greater could prevent hydrolytic degradation without sacrificing the vibrational behavior of the ME material. This work allows for long-term durability and functionality of ME materials in an aqueous and biological environment and makes the potential use of this technology in monitoring and modulating cellular behavior at the surface of implantable devices feasible.

No MeSH data available.


Related in: MedlinePlus

The Chemical Vapor Deposition process of Parylene-C. The dimer dichloro-di(p-xylylene) is cleaved into two chloro-p-xylylene monomer units and undergoes sublimation at temperatures about 550 °C and pressures less than 1 torr. The monomer units are then deposited onto a surface where they spontaneously polymerize at room temperature.
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biosensors-02-00057-f001: The Chemical Vapor Deposition process of Parylene-C. The dimer dichloro-di(p-xylylene) is cleaved into two chloro-p-xylylene monomer units and undergoes sublimation at temperatures about 550 °C and pressures less than 1 torr. The monomer units are then deposited onto a surface where they spontaneously polymerize at room temperature.

Mentions: For this study, we investigate the capabilities of poly-(chloro-p-xylylene), more commonly known as Parylene-C, as the biocompatible coating for magnetoelastic materials. Parylene-C is designated by the United States Pharmacopeia as a Class IV polymer, the highest level of biocompatibility for polymers, making it permissible for long-term implantation [18]. Films made from chemical vapor deposited Parylene-C possess many desirable properties for medical applications, including chemical inertness and resistance to biological degradation [19,20], and have already seen use in a variety of biomedical devices such as cardiovascular implants, wireless neural interfaces, and catheters [18,20]. Additionally, Parylene-C is often chosen for medical use due to its deposition process (Figure 1) [18,19,20,21]. Advantages of this deposition process include excellent adhesion, a solvent-free environment, and high accuracy and control over film thickness [21,22]. Ultimately this process results in coatings that are highly resistant to hydrolytic degradation and stable in a biological environment [18,19,21].


Fabrication of biocompatible, vibrational magnetoelastic materials for controlling cellular adhesion.

Holmes HR, Tan EL, Ong KG, Rajachar RM - Biosensors (Basel) (2012)

The Chemical Vapor Deposition process of Parylene-C. The dimer dichloro-di(p-xylylene) is cleaved into two chloro-p-xylylene monomer units and undergoes sublimation at temperatures about 550 °C and pressures less than 1 torr. The monomer units are then deposited onto a surface where they spontaneously polymerize at room temperature.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263540&req=5

biosensors-02-00057-f001: The Chemical Vapor Deposition process of Parylene-C. The dimer dichloro-di(p-xylylene) is cleaved into two chloro-p-xylylene monomer units and undergoes sublimation at temperatures about 550 °C and pressures less than 1 torr. The monomer units are then deposited onto a surface where they spontaneously polymerize at room temperature.
Mentions: For this study, we investigate the capabilities of poly-(chloro-p-xylylene), more commonly known as Parylene-C, as the biocompatible coating for magnetoelastic materials. Parylene-C is designated by the United States Pharmacopeia as a Class IV polymer, the highest level of biocompatibility for polymers, making it permissible for long-term implantation [18]. Films made from chemical vapor deposited Parylene-C possess many desirable properties for medical applications, including chemical inertness and resistance to biological degradation [19,20], and have already seen use in a variety of biomedical devices such as cardiovascular implants, wireless neural interfaces, and catheters [18,20]. Additionally, Parylene-C is often chosen for medical use due to its deposition process (Figure 1) [18,19,20,21]. Advantages of this deposition process include excellent adhesion, a solvent-free environment, and high accuracy and control over film thickness [21,22]. Ultimately this process results in coatings that are highly resistant to hydrolytic degradation and stable in a biological environment [18,19,21].

Bottom Line: However, since ME materials are not inherently biocompatible, surface modifications are needed for their implementation in biological settings.In vitro cytotoxicity measurement and characterization of the vibrational behavior of the ME materials showed that Parylene-C coatings of 10 µm or greater could prevent hydrolytic degradation without sacrificing the vibrational behavior of the ME material.This work allows for long-term durability and functionality of ME materials in an aqueous and biological environment and makes the potential use of this technology in monitoring and modulating cellular behavior at the surface of implantable devices feasible.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, Michigan Technological University, Houghton, MI 49931, USA. hrholmes@mtu.edu.

ABSTRACT
This paper describes the functionalization of magnetoelastic (ME) materials with Parylene-C coating to improve the surface reactivity to cellular response. Previous study has demonstrated that vibrating ME materials were capable of modulating cellular adhesion when activated by an externally applied AC magnetic field. However, since ME materials are not inherently biocompatible, surface modifications are needed for their implementation in biological settings. Here, the long-term stability of the ME material in an aqueous and biological environment is achieved by chemical-vapor deposition of a conformal Parylene-C layer, and further functionalized by methods of oxygen plasma etching and protein adsorption. In vitro cytotoxicity measurement and characterization of the vibrational behavior of the ME materials showed that Parylene-C coatings of 10 µm or greater could prevent hydrolytic degradation without sacrificing the vibrational behavior of the ME material. This work allows for long-term durability and functionality of ME materials in an aqueous and biological environment and makes the potential use of this technology in monitoring and modulating cellular behavior at the surface of implantable devices feasible.

No MeSH data available.


Related in: MedlinePlus