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Miniaturized quantum semiconductor surface plasmon resonance platform for detection of biological molecules.

Lepage D, Dubowski JJ - Biosensors (Basel) (2013)

Bottom Line: The SPR technology is already commonly used for biochemical characterization in pharmaceutical industries, but the reduction of the distance between the SP exciting source and the biosensing platform to a few hundreds of nanometers is an innovative approach enabling us to achieve an ultimate miniaturization of the device.We evaluate the signal quality of this nanophotonic QW-SPR device using hyperspectral-imaging technology, and we compare its performance with that of a standard prism-based commercial system.With an innovative conical method of SPR data collection, we demonstrate that individually collected SPR scan, each in less than 2.2 s, yield a resolution of the detection at 1.5 × 10-6 RIU.

View Article: PubMed Central - PubMed

Affiliation: Laboratory for Quantum Semiconductors and Photon-based BioNanotechnology, Interdisciplinary Institute for Technological Innovation (3IT), Faculty of Engineering, Université de Sherbrooke, 3000 boul. de l'Université, Sherbrooke, QC J1K 0A5, Canada. dominic.lepage@usherbrooke.ca.

ABSTRACT
The concept of a portable, inexpensive and semi-automated biosensing platform, or lab-on-a-chip, is a vision shared by many researchers and venture industries. Under this scope, we have investigated the application of optical emission from quantum well (QW) microstructures for monitoring surface phenomena on gold layers remaining in proximity (<300 nm) with QW microstructures. The uncollimated QW radiation excites surface plasmons (SP) and through the surface plasmon resonance (SPR) effect allows for detection of small perturbation in the density surface adsorbates. The SPR technology is already commonly used for biochemical characterization in pharmaceutical industries, but the reduction of the distance between the SP exciting source and the biosensing platform to a few hundreds of nanometers is an innovative approach enabling us to achieve an ultimate miniaturization of the device. We evaluate the signal quality of this nanophotonic QW-SPR device using hyperspectral-imaging technology, and we compare its performance with that of a standard prism-based commercial system. Two standard biochemical agents are employed for this characterization study: bovine serum albumin and inactivated influenza A virus. With an innovative conical method of SPR data collection, we demonstrate that individually collected SPR scan, each in less than 2.2 s, yield a resolution of the detection at 1.5 × 10-6 RIU.

No MeSH data available.


Related in: MedlinePlus

Detection of H3N2 IAV with a QW-SPR device. (a) ΔSPR as a function of E-ky and time, as the neutravidin (0 < t ≤ 230 min), biotinylated polyclonal IAV H3N2 antibodies (240 < t ≤ 420 min) and inactivated IAV H3N2 (t > 420 min) solutions are injected over the device and rinsed. (b) Cumulative SPR shift, ΔSPR/Total, in time for the IAV H3N2 adsorption. A surficial shift of Δs = 33 × 10−3 ± 1 × 10−3 µm−1 is measured after rinsing the surfaces with PBS [9].
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biosensors-03-00201-f004: Detection of H3N2 IAV with a QW-SPR device. (a) ΔSPR as a function of E-ky and time, as the neutravidin (0 < t ≤ 230 min), biotinylated polyclonal IAV H3N2 antibodies (240 < t ≤ 420 min) and inactivated IAV H3N2 (t > 420 min) solutions are injected over the device and rinsed. (b) Cumulative SPR shift, ΔSPR/Total, in time for the IAV H3N2 adsorption. A surficial shift of Δs = 33 × 10−3 ± 1 × 10−3 µm−1 is measured after rinsing the surfaces with PBS [9].

Mentions: Figure 3 shows a single frame (2.2 s collection time) result obtained with our approach during physisorption of BSA on the surface of a QW-SPR device [9]. The diffracted SP modes, represented by fragments of two ellipses, are clearly visible. Note that the quality of the signal as a function of E-ky depends on the luminescence intensity of the QW, which here is manifested by the increased noise amplitude of the top and bottom parts of the recorded frame. The information on the SPR conditions on the surface of the QW-SPR device can be obtained by measuring the distance between the ellipses as a function of time. In the case of a broadband and uncollimated SPR system, the shift occurs in E, kx and ky. Therefore, a cumulative shift between the two SPR peak surfaces corresponds to ΔSPR/Total= {℘ ΔSPR /E-ky/2}½. For BSA physisorption, we measured Δs = 7,140 × 10−4 ± 60 × 10−4 µm−1 after the PBS rinse. For the specific immobilization of IAV H3N2, the dynamic variations of ΔSPR/Total are presented in Figure 4. Note that the periodic modulations in E-ky (Figure 4(a)) are attributed to the interactions of the SPs with the grating as predicted in the literature [13,15,16]. In this case, the shift induced by the selective immobilization of H3N2 is Δs = 33 × 10−3 ± 1 × 10−3 µm−1 after the PBS rinse. For this system, the resulting signal to noise ratio was found to be SNR = 1,831 ± 12. When comparing the SNR of the SPR signal generated by the QW-SPR architecture with that of the commercial system, we find the former generates slightly more stable datasets. This is mainly attributed to the continuous surface coupling of the SPR over a wide range of E and kll, resulting in the cumulative shifts over the collected dispersions that are greater than the potential shift over a single variable in E or kll values. In this hyperspectral acquisition mode, the shot noise of the system is thus reduced by upsampling the number of monitored SPR events (in E and kll in time).


Miniaturized quantum semiconductor surface plasmon resonance platform for detection of biological molecules.

Lepage D, Dubowski JJ - Biosensors (Basel) (2013)

Detection of H3N2 IAV with a QW-SPR device. (a) ΔSPR as a function of E-ky and time, as the neutravidin (0 < t ≤ 230 min), biotinylated polyclonal IAV H3N2 antibodies (240 < t ≤ 420 min) and inactivated IAV H3N2 (t > 420 min) solutions are injected over the device and rinsed. (b) Cumulative SPR shift, ΔSPR/Total, in time for the IAV H3N2 adsorption. A surficial shift of Δs = 33 × 10−3 ± 1 × 10−3 µm−1 is measured after rinsing the surfaces with PBS [9].
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263536&req=5

biosensors-03-00201-f004: Detection of H3N2 IAV with a QW-SPR device. (a) ΔSPR as a function of E-ky and time, as the neutravidin (0 < t ≤ 230 min), biotinylated polyclonal IAV H3N2 antibodies (240 < t ≤ 420 min) and inactivated IAV H3N2 (t > 420 min) solutions are injected over the device and rinsed. (b) Cumulative SPR shift, ΔSPR/Total, in time for the IAV H3N2 adsorption. A surficial shift of Δs = 33 × 10−3 ± 1 × 10−3 µm−1 is measured after rinsing the surfaces with PBS [9].
Mentions: Figure 3 shows a single frame (2.2 s collection time) result obtained with our approach during physisorption of BSA on the surface of a QW-SPR device [9]. The diffracted SP modes, represented by fragments of two ellipses, are clearly visible. Note that the quality of the signal as a function of E-ky depends on the luminescence intensity of the QW, which here is manifested by the increased noise amplitude of the top and bottom parts of the recorded frame. The information on the SPR conditions on the surface of the QW-SPR device can be obtained by measuring the distance between the ellipses as a function of time. In the case of a broadband and uncollimated SPR system, the shift occurs in E, kx and ky. Therefore, a cumulative shift between the two SPR peak surfaces corresponds to ΔSPR/Total= {℘ ΔSPR /E-ky/2}½. For BSA physisorption, we measured Δs = 7,140 × 10−4 ± 60 × 10−4 µm−1 after the PBS rinse. For the specific immobilization of IAV H3N2, the dynamic variations of ΔSPR/Total are presented in Figure 4. Note that the periodic modulations in E-ky (Figure 4(a)) are attributed to the interactions of the SPs with the grating as predicted in the literature [13,15,16]. In this case, the shift induced by the selective immobilization of H3N2 is Δs = 33 × 10−3 ± 1 × 10−3 µm−1 after the PBS rinse. For this system, the resulting signal to noise ratio was found to be SNR = 1,831 ± 12. When comparing the SNR of the SPR signal generated by the QW-SPR architecture with that of the commercial system, we find the former generates slightly more stable datasets. This is mainly attributed to the continuous surface coupling of the SPR over a wide range of E and kll, resulting in the cumulative shifts over the collected dispersions that are greater than the potential shift over a single variable in E or kll values. In this hyperspectral acquisition mode, the shot noise of the system is thus reduced by upsampling the number of monitored SPR events (in E and kll in time).

Bottom Line: The SPR technology is already commonly used for biochemical characterization in pharmaceutical industries, but the reduction of the distance between the SP exciting source and the biosensing platform to a few hundreds of nanometers is an innovative approach enabling us to achieve an ultimate miniaturization of the device.We evaluate the signal quality of this nanophotonic QW-SPR device using hyperspectral-imaging technology, and we compare its performance with that of a standard prism-based commercial system.With an innovative conical method of SPR data collection, we demonstrate that individually collected SPR scan, each in less than 2.2 s, yield a resolution of the detection at 1.5 × 10-6 RIU.

View Article: PubMed Central - PubMed

Affiliation: Laboratory for Quantum Semiconductors and Photon-based BioNanotechnology, Interdisciplinary Institute for Technological Innovation (3IT), Faculty of Engineering, Université de Sherbrooke, 3000 boul. de l'Université, Sherbrooke, QC J1K 0A5, Canada. dominic.lepage@usherbrooke.ca.

ABSTRACT
The concept of a portable, inexpensive and semi-automated biosensing platform, or lab-on-a-chip, is a vision shared by many researchers and venture industries. Under this scope, we have investigated the application of optical emission from quantum well (QW) microstructures for monitoring surface phenomena on gold layers remaining in proximity (<300 nm) with QW microstructures. The uncollimated QW radiation excites surface plasmons (SP) and through the surface plasmon resonance (SPR) effect allows for detection of small perturbation in the density surface adsorbates. The SPR technology is already commonly used for biochemical characterization in pharmaceutical industries, but the reduction of the distance between the SP exciting source and the biosensing platform to a few hundreds of nanometers is an innovative approach enabling us to achieve an ultimate miniaturization of the device. We evaluate the signal quality of this nanophotonic QW-SPR device using hyperspectral-imaging technology, and we compare its performance with that of a standard prism-based commercial system. Two standard biochemical agents are employed for this characterization study: bovine serum albumin and inactivated influenza A virus. With an innovative conical method of SPR data collection, we demonstrate that individually collected SPR scan, each in less than 2.2 s, yield a resolution of the detection at 1.5 × 10-6 RIU.

No MeSH data available.


Related in: MedlinePlus