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Miniaturized quantum semiconductor surface plasmon resonance platform for detection of biological molecules.

Lepage D, Dubowski JJ - Biosensors (Basel) (2013)

Bottom Line: The SPR technology is already commonly used for biochemical characterization in pharmaceutical industries, but the reduction of the distance between the SP exciting source and the biosensing platform to a few hundreds of nanometers is an innovative approach enabling us to achieve an ultimate miniaturization of the device.We evaluate the signal quality of this nanophotonic QW-SPR device using hyperspectral-imaging technology, and we compare its performance with that of a standard prism-based commercial system.With an innovative conical method of SPR data collection, we demonstrate that individually collected SPR scan, each in less than 2.2 s, yield a resolution of the detection at 1.5 × 10-6 RIU.

View Article: PubMed Central - PubMed

Affiliation: Laboratory for Quantum Semiconductors and Photon-based BioNanotechnology, Interdisciplinary Institute for Technological Innovation (3IT), Faculty of Engineering, Université de Sherbrooke, 3000 boul. de l'Université, Sherbrooke, QC J1K 0A5, Canada. dominic.lepage@usherbrooke.ca.

ABSTRACT
The concept of a portable, inexpensive and semi-automated biosensing platform, or lab-on-a-chip, is a vision shared by many researchers and venture industries. Under this scope, we have investigated the application of optical emission from quantum well (QW) microstructures for monitoring surface phenomena on gold layers remaining in proximity (<300 nm) with QW microstructures. The uncollimated QW radiation excites surface plasmons (SP) and through the surface plasmon resonance (SPR) effect allows for detection of small perturbation in the density surface adsorbates. The SPR technology is already commonly used for biochemical characterization in pharmaceutical industries, but the reduction of the distance between the SP exciting source and the biosensing platform to a few hundreds of nanometers is an innovative approach enabling us to achieve an ultimate miniaturization of the device. We evaluate the signal quality of this nanophotonic QW-SPR device using hyperspectral-imaging technology, and we compare its performance with that of a standard prism-based commercial system. Two standard biochemical agents are employed for this characterization study: bovine serum albumin and inactivated influenza A virus. With an innovative conical method of SPR data collection, we demonstrate that individually collected SPR scan, each in less than 2.2 s, yield a resolution of the detection at 1.5 × 10-6 RIU.

No MeSH data available.


Related in: MedlinePlus

(a) Broadband uncollimated induction of SPR. At a given energy, E, and specific wavevector kll = kSPR, electromagnetic charge fluctuations in the form of surface plasmons can be coupled at a metal-dielectric interface. Changing the input energy will modify the required kll to couple a resonance. As such, there is a continuous dispersion relation E(kSPR) for the resonant coupling of the surface plasmons (inset); (b) Since kll can take any direction (, ) on the surface, the SPR dispersion in the E(kx, ky) space generates a cone-like shape surface. Note that under normal circumstances kSPR > klight. This SPR dispersion is thus scattered within the light cone by the surface corrugation, through the ±1st diffraction order of a grating for example, to be measured by a microscope in the far field [9,13].
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biosensors-03-00201-f002: (a) Broadband uncollimated induction of SPR. At a given energy, E, and specific wavevector kll = kSPR, electromagnetic charge fluctuations in the form of surface plasmons can be coupled at a metal-dielectric interface. Changing the input energy will modify the required kll to couple a resonance. As such, there is a continuous dispersion relation E(kSPR) for the resonant coupling of the surface plasmons (inset); (b) Since kll can take any direction (, ) on the surface, the SPR dispersion in the E(kx, ky) space generates a cone-like shape surface. Note that under normal circumstances kSPR > klight. This SPR dispersion is thus scattered within the light cone by the surface corrugation, through the ±1st diffraction order of a grating for example, to be measured by a microscope in the far field [9,13].

Mentions: An integration of the SPR technology with a light emitting semiconductor is achieved by constructing a metal-dielectric interface atop the solid-state light emitting substrate [2,6,7,8], as shown in Figure 1 [9]. Through electroluminescence or photoluminescence (PL), the embedded quantum well (QW) is excited to emit radiation. For the most general case presented here, the emission is uncollimated (i.e., propagating in all directions) and the broadband spectral bandwidth exceeds 140 nm (1.38–1.65 eV). A dielectric spacer and metal layer are deposited by evaporation on the structure, thus enabling the support of surface plasmon (SP) modes. The metal layer is corrugated in order to extract the SPs into the far field for imaging by a microscope. This top layer can also be biofunctionalized in various manners for specific experiments. The principle behind uncollimated and broadband SP coupling is detailed in Figure 2 [9]. A continuous range of incoming radiation from the QW encounters the metal layer at the dielectric interface.


Miniaturized quantum semiconductor surface plasmon resonance platform for detection of biological molecules.

Lepage D, Dubowski JJ - Biosensors (Basel) (2013)

(a) Broadband uncollimated induction of SPR. At a given energy, E, and specific wavevector kll = kSPR, electromagnetic charge fluctuations in the form of surface plasmons can be coupled at a metal-dielectric interface. Changing the input energy will modify the required kll to couple a resonance. As such, there is a continuous dispersion relation E(kSPR) for the resonant coupling of the surface plasmons (inset); (b) Since kll can take any direction (, ) on the surface, the SPR dispersion in the E(kx, ky) space generates a cone-like shape surface. Note that under normal circumstances kSPR > klight. This SPR dispersion is thus scattered within the light cone by the surface corrugation, through the ±1st diffraction order of a grating for example, to be measured by a microscope in the far field [9,13].
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263536&req=5

biosensors-03-00201-f002: (a) Broadband uncollimated induction of SPR. At a given energy, E, and specific wavevector kll = kSPR, electromagnetic charge fluctuations in the form of surface plasmons can be coupled at a metal-dielectric interface. Changing the input energy will modify the required kll to couple a resonance. As such, there is a continuous dispersion relation E(kSPR) for the resonant coupling of the surface plasmons (inset); (b) Since kll can take any direction (, ) on the surface, the SPR dispersion in the E(kx, ky) space generates a cone-like shape surface. Note that under normal circumstances kSPR > klight. This SPR dispersion is thus scattered within the light cone by the surface corrugation, through the ±1st diffraction order of a grating for example, to be measured by a microscope in the far field [9,13].
Mentions: An integration of the SPR technology with a light emitting semiconductor is achieved by constructing a metal-dielectric interface atop the solid-state light emitting substrate [2,6,7,8], as shown in Figure 1 [9]. Through electroluminescence or photoluminescence (PL), the embedded quantum well (QW) is excited to emit radiation. For the most general case presented here, the emission is uncollimated (i.e., propagating in all directions) and the broadband spectral bandwidth exceeds 140 nm (1.38–1.65 eV). A dielectric spacer and metal layer are deposited by evaporation on the structure, thus enabling the support of surface plasmon (SP) modes. The metal layer is corrugated in order to extract the SPs into the far field for imaging by a microscope. This top layer can also be biofunctionalized in various manners for specific experiments. The principle behind uncollimated and broadband SP coupling is detailed in Figure 2 [9]. A continuous range of incoming radiation from the QW encounters the metal layer at the dielectric interface.

Bottom Line: The SPR technology is already commonly used for biochemical characterization in pharmaceutical industries, but the reduction of the distance between the SP exciting source and the biosensing platform to a few hundreds of nanometers is an innovative approach enabling us to achieve an ultimate miniaturization of the device.We evaluate the signal quality of this nanophotonic QW-SPR device using hyperspectral-imaging technology, and we compare its performance with that of a standard prism-based commercial system.With an innovative conical method of SPR data collection, we demonstrate that individually collected SPR scan, each in less than 2.2 s, yield a resolution of the detection at 1.5 × 10-6 RIU.

View Article: PubMed Central - PubMed

Affiliation: Laboratory for Quantum Semiconductors and Photon-based BioNanotechnology, Interdisciplinary Institute for Technological Innovation (3IT), Faculty of Engineering, Université de Sherbrooke, 3000 boul. de l'Université, Sherbrooke, QC J1K 0A5, Canada. dominic.lepage@usherbrooke.ca.

ABSTRACT
The concept of a portable, inexpensive and semi-automated biosensing platform, or lab-on-a-chip, is a vision shared by many researchers and venture industries. Under this scope, we have investigated the application of optical emission from quantum well (QW) microstructures for monitoring surface phenomena on gold layers remaining in proximity (<300 nm) with QW microstructures. The uncollimated QW radiation excites surface plasmons (SP) and through the surface plasmon resonance (SPR) effect allows for detection of small perturbation in the density surface adsorbates. The SPR technology is already commonly used for biochemical characterization in pharmaceutical industries, but the reduction of the distance between the SP exciting source and the biosensing platform to a few hundreds of nanometers is an innovative approach enabling us to achieve an ultimate miniaturization of the device. We evaluate the signal quality of this nanophotonic QW-SPR device using hyperspectral-imaging technology, and we compare its performance with that of a standard prism-based commercial system. Two standard biochemical agents are employed for this characterization study: bovine serum albumin and inactivated influenza A virus. With an innovative conical method of SPR data collection, we demonstrate that individually collected SPR scan, each in less than 2.2 s, yield a resolution of the detection at 1.5 × 10-6 RIU.

No MeSH data available.


Related in: MedlinePlus