Prevalence and phylogenetic analysis of haemoplasmas from cats infected with multiple species.
Bottom Line: Male gender was significantly associated with haemoplasma infections.No association was found between qPCR haemoplasma status and haematological variables, however CMhm relative copy numbers were correlated with red blood cell (RBC) numbers and packed cell volume (PCV).These phylogeny findings suggest the existence of different CMhm and CMt strains.
Affiliation: Laboratory of Veterinary Clinical Pathology, College of Agronomy and Veterinary Medicine, University of Brasília, Campus Universitário Darcy Ribeiro, Brasília 709010-900, Brazil. email@example.comShow MeSH
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Mentions: The phylogenetic relationships of the sequences generated in this study and those available on GenBank are shown in Fig. 2. This analysis yielded the expected separation of Mhf, CMhm and CMt sequences into three distinct clades accompanied by high bootstrap values. The Mhf sequences all grouped into one single clade with other worldwide Mhf sequences from both domestic and wild cats. However, grouping of the CMhm sequences into three distinct subclades was seen; subclades one and two compromised eight of the ten CMhm sequences from this study and were most closely related to wild cat haemoplasma sequences from Africa, Brazil and Spain; and subclade three consisted of two sequences that shared a closer evolutionary relationship with sequences from domestic cats in Europe (UK and Switzerland). The CMt phylogenetic analysis also showed division into three subclades; five of the 11 CMt sequences from this study grouped into subclades one and two, which were more closely related to sequences from domestic cats in Australia and Africa, whereas the CMt sequences in subclade three were more closely related to domestic and wild cat sequences from European countries (Switzerland, UK, France).
Affiliation: Laboratory of Veterinary Clinical Pathology, College of Agronomy and Veterinary Medicine, University of Brasília, Campus Universitário Darcy Ribeiro, Brasília 709010-900, Brazil. firstname.lastname@example.org