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Prevalence and phylogenetic analysis of haemoplasmas from cats infected with multiple species.

Aquino LC, Hicks CA, Scalon MC, Lima MG, Lemos Mdos S, Paludo GR, Helps CR, Tasker S - J. Microbiol. Methods (2014)

Bottom Line: Male gender was significantly associated with haemoplasma infections.No association was found between qPCR haemoplasma status and haematological variables, however CMhm relative copy numbers were correlated with red blood cell (RBC) numbers and packed cell volume (PCV).These phylogeny findings suggest the existence of different CMhm and CMt strains.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Veterinary Clinical Pathology, College of Agronomy and Veterinary Medicine, University of Brasília, Campus Universitário Darcy Ribeiro, Brasília 709010-900, Brazil. larissaquino@gmail.com

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Phylogenetic analysis of near complete 16S rRNA gene sequences from Mhf, CMhm and CMt species in cats from Brasília and surrounding areas. The Neighbor-joining method was used to construct the phylogenetic tree with bootstrap values provided at the nodes (only values ≥ 700 are shown). Evolutionary distances are to the scales shown. Clostridium spp. was used as an out-group. GenBank accession numbers are indicated in the figure. Mhf sequences (B6, B7, C6, D4, E7, F2, F3, F5, G4, H2), CMhm sequences (A8, B4, B5, B8, D1, D2, D4, D5, E3, H4) and CMt sequences (B1, B2, D4, E7, F3, F4, F5, G1, G4, G5, H4) were generated in the current study. Mhf = Mycoplasma haemofelis, CMhm = “Candidatus Mycoplasma haemominutum”, CMt = “Candidatus Mycoplasma turicensis”.
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f0010: Phylogenetic analysis of near complete 16S rRNA gene sequences from Mhf, CMhm and CMt species in cats from Brasília and surrounding areas. The Neighbor-joining method was used to construct the phylogenetic tree with bootstrap values provided at the nodes (only values ≥ 700 are shown). Evolutionary distances are to the scales shown. Clostridium spp. was used as an out-group. GenBank accession numbers are indicated in the figure. Mhf sequences (B6, B7, C6, D4, E7, F2, F3, F5, G4, H2), CMhm sequences (A8, B4, B5, B8, D1, D2, D4, D5, E3, H4) and CMt sequences (B1, B2, D4, E7, F3, F4, F5, G1, G4, G5, H4) were generated in the current study. Mhf = Mycoplasma haemofelis, CMhm = “Candidatus Mycoplasma haemominutum”, CMt = “Candidatus Mycoplasma turicensis”.

Mentions: The phylogenetic relationships of the sequences generated in this study and those available on GenBank are shown in Fig. 2. This analysis yielded the expected separation of Mhf, CMhm and CMt sequences into three distinct clades accompanied by high bootstrap values. The Mhf sequences all grouped into one single clade with other worldwide Mhf sequences from both domestic and wild cats. However, grouping of the CMhm sequences into three distinct subclades was seen; subclades one and two compromised eight of the ten CMhm sequences from this study and were most closely related to wild cat haemoplasma sequences from Africa, Brazil and Spain; and subclade three consisted of two sequences that shared a closer evolutionary relationship with sequences from domestic cats in Europe (UK and Switzerland). The CMt phylogenetic analysis also showed division into three subclades; five of the 11 CMt sequences from this study grouped into subclades one and two, which were more closely related to sequences from domestic cats in Australia and Africa, whereas the CMt sequences in subclade three were more closely related to domestic and wild cat sequences from European countries (Switzerland, UK, France).


Prevalence and phylogenetic analysis of haemoplasmas from cats infected with multiple species.

Aquino LC, Hicks CA, Scalon MC, Lima MG, Lemos Mdos S, Paludo GR, Helps CR, Tasker S - J. Microbiol. Methods (2014)

Phylogenetic analysis of near complete 16S rRNA gene sequences from Mhf, CMhm and CMt species in cats from Brasília and surrounding areas. The Neighbor-joining method was used to construct the phylogenetic tree with bootstrap values provided at the nodes (only values ≥ 700 are shown). Evolutionary distances are to the scales shown. Clostridium spp. was used as an out-group. GenBank accession numbers are indicated in the figure. Mhf sequences (B6, B7, C6, D4, E7, F2, F3, F5, G4, H2), CMhm sequences (A8, B4, B5, B8, D1, D2, D4, D5, E3, H4) and CMt sequences (B1, B2, D4, E7, F3, F4, F5, G1, G4, G5, H4) were generated in the current study. Mhf = Mycoplasma haemofelis, CMhm = “Candidatus Mycoplasma haemominutum”, CMt = “Candidatus Mycoplasma turicensis”.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4263531&req=5

f0010: Phylogenetic analysis of near complete 16S rRNA gene sequences from Mhf, CMhm and CMt species in cats from Brasília and surrounding areas. The Neighbor-joining method was used to construct the phylogenetic tree with bootstrap values provided at the nodes (only values ≥ 700 are shown). Evolutionary distances are to the scales shown. Clostridium spp. was used as an out-group. GenBank accession numbers are indicated in the figure. Mhf sequences (B6, B7, C6, D4, E7, F2, F3, F5, G4, H2), CMhm sequences (A8, B4, B5, B8, D1, D2, D4, D5, E3, H4) and CMt sequences (B1, B2, D4, E7, F3, F4, F5, G1, G4, G5, H4) were generated in the current study. Mhf = Mycoplasma haemofelis, CMhm = “Candidatus Mycoplasma haemominutum”, CMt = “Candidatus Mycoplasma turicensis”.
Mentions: The phylogenetic relationships of the sequences generated in this study and those available on GenBank are shown in Fig. 2. This analysis yielded the expected separation of Mhf, CMhm and CMt sequences into three distinct clades accompanied by high bootstrap values. The Mhf sequences all grouped into one single clade with other worldwide Mhf sequences from both domestic and wild cats. However, grouping of the CMhm sequences into three distinct subclades was seen; subclades one and two compromised eight of the ten CMhm sequences from this study and were most closely related to wild cat haemoplasma sequences from Africa, Brazil and Spain; and subclade three consisted of two sequences that shared a closer evolutionary relationship with sequences from domestic cats in Europe (UK and Switzerland). The CMt phylogenetic analysis also showed division into three subclades; five of the 11 CMt sequences from this study grouped into subclades one and two, which were more closely related to sequences from domestic cats in Australia and Africa, whereas the CMt sequences in subclade three were more closely related to domestic and wild cat sequences from European countries (Switzerland, UK, France).

Bottom Line: Male gender was significantly associated with haemoplasma infections.No association was found between qPCR haemoplasma status and haematological variables, however CMhm relative copy numbers were correlated with red blood cell (RBC) numbers and packed cell volume (PCV).These phylogeny findings suggest the existence of different CMhm and CMt strains.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Veterinary Clinical Pathology, College of Agronomy and Veterinary Medicine, University of Brasília, Campus Universitário Darcy Ribeiro, Brasília 709010-900, Brazil. larissaquino@gmail.com

Show MeSH
Related in: MedlinePlus