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T-helper 1 immunoreaction influences survival in muscle-invasive bladder cancer: proof of concept.

Ingels A, Sanchez Salas RE, Ravery V, Fromont-Hankard G, Validire P, Patard JJ, Pignot G, Prapotnich D, Olivier F, Galiano M, Barret E, Rozet F, Weber N, Cathelineau X - Ecancermedicalscience (2014)

Bottom Line: Survivors harboured higher lymphocytes densities versus non survivors (CD3: p = 0.0319; CD8: p = 0.0279).CD3 (p = 0.034) and CD8 (p = 0.034) lymphocytes densities were independently associated with cancer-specific survival on Cox model analyses.High CD3 and CD8 lymphocytes SR densities are associated with better cancer-specific survival for MIBC.

View Article: PubMed Central - PubMed

Affiliation: Institut Montsouris, Department of Urology, 42 Boulevard Jourdan, 75014 Paris, France.

ABSTRACT

Objective: To define immunoscore in bladder cancer studying T helper 1 (Th1) immunoreaction. To define a cancer-specific survival model based on Th1 cells infiltration.

Methods: A total of 252 patients underwent primary transurethral resection of bladder tumour at our Institution. A retrospective review of a selected cohort with pT1 and muscle-invasive bladder cancer (MIBC) lesions was performed. Pathology blocks were marked with CD3 and CD8 antibodies. Immune cells density in stromal reaction (SR) was measured on five distinct high-power field (HPF) by two dedicated uro-pathologist blinded for patients' evolution.

Statistics: Student test or non-parametric Wilcoxon test as appropriate to compare means between two groups. Receiver operating characteristics (ROC) curve to define markers threshold. Cox model to assess survival's predictors.

Results: Ten pT1 and 20 MIBC consecutive cases were analysed. Median follow-up was 33.4 months. Immunohistological analysis for pT1 lesions featured limited SR. For MIBC, the mean density of lymphocytes in the SR was of 105/HPF (CD3) and 86/HPF (CD8). Survivors harboured higher lymphocytes densities versus non survivors (CD3: p = 0.0319; CD8: p = 0.0279). CD3 (p = 0.034) and CD8 (p = 0.034) lymphocytes densities were independently associated with cancer-specific survival on Cox model analyses. The retrospective design and small size of cohorts are the study limitations.

Conclusions: High CD3 and CD8 lymphocytes SR densities are associated with better cancer-specific survival for MIBC. Th1 reaction against the tumour seems to be protective for bladder cancer. Further evaluation is warranted.

No MeSH data available.


Related in: MedlinePlus

Immunostaining of the CD3 (left) and CD8 (right) cells infiltration into the peri-tumoural stroma (magnification 40 x 10). The cell density is counted in five distinctive fields. The final value is the five figures’ mean.
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figure1: Immunostaining of the CD3 (left) and CD8 (right) cells infiltration into the peri-tumoural stroma (magnification 40 x 10). The cell density is counted in five distinctive fields. The final value is the five figures’ mean.

Mentions: CD3 and CD8 TILS were detected in both SR and epithelial tumour nests (Figure 1). As stated previously, we decided to focus only on the SR.


T-helper 1 immunoreaction influences survival in muscle-invasive bladder cancer: proof of concept.

Ingels A, Sanchez Salas RE, Ravery V, Fromont-Hankard G, Validire P, Patard JJ, Pignot G, Prapotnich D, Olivier F, Galiano M, Barret E, Rozet F, Weber N, Cathelineau X - Ecancermedicalscience (2014)

Immunostaining of the CD3 (left) and CD8 (right) cells infiltration into the peri-tumoural stroma (magnification 40 x 10). The cell density is counted in five distinctive fields. The final value is the five figures’ mean.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263522&req=5

figure1: Immunostaining of the CD3 (left) and CD8 (right) cells infiltration into the peri-tumoural stroma (magnification 40 x 10). The cell density is counted in five distinctive fields. The final value is the five figures’ mean.
Mentions: CD3 and CD8 TILS were detected in both SR and epithelial tumour nests (Figure 1). As stated previously, we decided to focus only on the SR.

Bottom Line: Survivors harboured higher lymphocytes densities versus non survivors (CD3: p = 0.0319; CD8: p = 0.0279).CD3 (p = 0.034) and CD8 (p = 0.034) lymphocytes densities were independently associated with cancer-specific survival on Cox model analyses.High CD3 and CD8 lymphocytes SR densities are associated with better cancer-specific survival for MIBC.

View Article: PubMed Central - PubMed

Affiliation: Institut Montsouris, Department of Urology, 42 Boulevard Jourdan, 75014 Paris, France.

ABSTRACT

Objective: To define immunoscore in bladder cancer studying T helper 1 (Th1) immunoreaction. To define a cancer-specific survival model based on Th1 cells infiltration.

Methods: A total of 252 patients underwent primary transurethral resection of bladder tumour at our Institution. A retrospective review of a selected cohort with pT1 and muscle-invasive bladder cancer (MIBC) lesions was performed. Pathology blocks were marked with CD3 and CD8 antibodies. Immune cells density in stromal reaction (SR) was measured on five distinct high-power field (HPF) by two dedicated uro-pathologist blinded for patients' evolution.

Statistics: Student test or non-parametric Wilcoxon test as appropriate to compare means between two groups. Receiver operating characteristics (ROC) curve to define markers threshold. Cox model to assess survival's predictors.

Results: Ten pT1 and 20 MIBC consecutive cases were analysed. Median follow-up was 33.4 months. Immunohistological analysis for pT1 lesions featured limited SR. For MIBC, the mean density of lymphocytes in the SR was of 105/HPF (CD3) and 86/HPF (CD8). Survivors harboured higher lymphocytes densities versus non survivors (CD3: p = 0.0319; CD8: p = 0.0279). CD3 (p = 0.034) and CD8 (p = 0.034) lymphocytes densities were independently associated with cancer-specific survival on Cox model analyses. The retrospective design and small size of cohorts are the study limitations.

Conclusions: High CD3 and CD8 lymphocytes SR densities are associated with better cancer-specific survival for MIBC. Th1 reaction against the tumour seems to be protective for bladder cancer. Further evaluation is warranted.

No MeSH data available.


Related in: MedlinePlus