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Impact of individual extracellular proteases on Staphylococcus aureus biofilm formation in diverse clinical isolates and their isogenic sarA mutants.

Loughran AJ, Atwood DN, Anthony AC, Harik NS, Spencer HJ, Beenken KE, Smeltzer MS - Microbiologyopen (2014)

Bottom Line: These results confirm an important role for multiple extracellular proteases in S. aureus pathogenesis and the importance of sarA in repressing their production.Moreover, purified aureolysin limited biofilm formation in 14 of 15 methicillin-resistant isolates and 11 of 15 methicillin-susceptible isolates, while dispersin B had little impact in UAMS-1, LAC, or 29 of 30 contemporary isolates of S. aureus.This suggests that the role of sarA and its impact on protease production is important in diverse strains of S. aureus irrespective of their methicillin resistance status.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, Arkansas.

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Impact of extracellular purified proteases on dispersal of established biofilms. Biofilms were allowed to form with the LAC protease-deficient mutant for 24 h before adding purified proteases in concentrations ranging from 250 to 16 nmol/L. The impact of each protease on dispersal of the established biofilm was then assessed 24 h later as previously described (Beenken et al. 2010). “C” indicates the control in which no exogenous protease was added. Asterisks indicate the lowest concentration of each protease at which a statistically significant difference was observed relative to this control.
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fig05: Impact of extracellular purified proteases on dispersal of established biofilms. Biofilms were allowed to form with the LAC protease-deficient mutant for 24 h before adding purified proteases in concentrations ranging from 250 to 16 nmol/L. The impact of each protease on dispersal of the established biofilm was then assessed 24 h later as previously described (Beenken et al. 2010). “C” indicates the control in which no exogenous protease was added. Asterisks indicate the lowest concentration of each protease at which a statistically significant difference was observed relative to this control.

Mentions: Consistent with these results, we also demonstrated that purified aureolysin had the greatest capacity to promote dispersal of an established biofilm formed by the protease-deficient derivative of LAC (Fig. 5), thus confirming an important role for aureolysin itself rather than its role as a primary mediator of the protease activation cascade. ScpA and SspB were also capable of promoting biofilm dispersal in this strain, while purified SspA had little effect (Fig. 5).


Impact of individual extracellular proteases on Staphylococcus aureus biofilm formation in diverse clinical isolates and their isogenic sarA mutants.

Loughran AJ, Atwood DN, Anthony AC, Harik NS, Spencer HJ, Beenken KE, Smeltzer MS - Microbiologyopen (2014)

Impact of extracellular purified proteases on dispersal of established biofilms. Biofilms were allowed to form with the LAC protease-deficient mutant for 24 h before adding purified proteases in concentrations ranging from 250 to 16 nmol/L. The impact of each protease on dispersal of the established biofilm was then assessed 24 h later as previously described (Beenken et al. 2010). “C” indicates the control in which no exogenous protease was added. Asterisks indicate the lowest concentration of each protease at which a statistically significant difference was observed relative to this control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263513&req=5

fig05: Impact of extracellular purified proteases on dispersal of established biofilms. Biofilms were allowed to form with the LAC protease-deficient mutant for 24 h before adding purified proteases in concentrations ranging from 250 to 16 nmol/L. The impact of each protease on dispersal of the established biofilm was then assessed 24 h later as previously described (Beenken et al. 2010). “C” indicates the control in which no exogenous protease was added. Asterisks indicate the lowest concentration of each protease at which a statistically significant difference was observed relative to this control.
Mentions: Consistent with these results, we also demonstrated that purified aureolysin had the greatest capacity to promote dispersal of an established biofilm formed by the protease-deficient derivative of LAC (Fig. 5), thus confirming an important role for aureolysin itself rather than its role as a primary mediator of the protease activation cascade. ScpA and SspB were also capable of promoting biofilm dispersal in this strain, while purified SspA had little effect (Fig. 5).

Bottom Line: These results confirm an important role for multiple extracellular proteases in S. aureus pathogenesis and the importance of sarA in repressing their production.Moreover, purified aureolysin limited biofilm formation in 14 of 15 methicillin-resistant isolates and 11 of 15 methicillin-susceptible isolates, while dispersin B had little impact in UAMS-1, LAC, or 29 of 30 contemporary isolates of S. aureus.This suggests that the role of sarA and its impact on protease production is important in diverse strains of S. aureus irrespective of their methicillin resistance status.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, Arkansas.

Show MeSH
Related in: MedlinePlus