Impact of individual extracellular proteases on Staphylococcus aureus biofilm formation in diverse clinical isolates and their isogenic sarA mutants.
Bottom Line: These results confirm an important role for multiple extracellular proteases in S. aureus pathogenesis and the importance of sarA in repressing their production.Moreover, purified aureolysin limited biofilm formation in 14 of 15 methicillin-resistant isolates and 11 of 15 methicillin-susceptible isolates, while dispersin B had little impact in UAMS-1, LAC, or 29 of 30 contemporary isolates of S. aureus.This suggests that the role of sarA and its impact on protease production is important in diverse strains of S. aureus irrespective of their methicillin resistance status.
Affiliation: Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, Arkansas.Show MeSH
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Mentions: Consistent with these results, we also demonstrated that purified aureolysin had the greatest capacity to promote dispersal of an established biofilm formed by the protease-deficient derivative of LAC (Fig. 5), thus confirming an important role for aureolysin itself rather than its role as a primary mediator of the protease activation cascade. ScpA and SspB were also capable of promoting biofilm dispersal in this strain, while purified SspA had little effect (Fig. 5).
Affiliation: Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, Arkansas.