O serotype-independent susceptibility of Pseudomonas aeruginosa to lectin-like pyocins.
Bottom Line: The recombinant proteins exhibit species-specific antagonistic activities down to nanomolar concentrations against clinical and environmental P. aeruginosa strains, including several multidrug-resistant isolates.No correlation was found between L pyocin susceptibility and phylogenetic relatedness of P. aeruginosa isolates.Sensitive strains were retrieved in 13 out of 15 O serotypes tested, excluding the possibility that the highly variable and immunogenic O serotype antigen of the LPS coating would represent a dominant susceptibility-discriminating factor.
Affiliation: Centre of Microbial and Plant Genetics, University of Leuven, 3001, Heverlee, Belgium.Show MeSH
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Mentions: Seventy strains in the test panel were selected from a collection of 328 P. aeruginosa isolates, based on their distribution across a MST previously built from a polyphasic data set (Pirnay et al. 2009). Characteristics include O serotype, fluorescent amplified-fragment length polymorphism pattern, gene sequences of outer membrane proteins (oprI, oprL, and oprD), pyoverdine receptors (fpvA and fpvB), group I pilin glycosyltransferase (tfpO), and the prevalence of exoenzyme genes (exoS and exoU). Patterns of pyocin sensitivity did not correlate with any of these characteristics, as variation occurred even within clonal complexes (Fig. 6A). Susceptible strains were found in virtually all MST clusters and, hence, liability to pyocin L-mediated killing cannot be attributed to phylogenetic relatedness, suggesting that (a) highly variable epitope(s) mainly determine(s) strain victimization.
Affiliation: Centre of Microbial and Plant Genetics, University of Leuven, 3001, Heverlee, Belgium.