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Multiple approaches to microbial source tracking in tropical northern Australia.

Neave M, Luter H, Padovan A, Townsend S, Schobben X, Gibb K - Microbiologyopen (2014)

Bottom Line: To address this, we sampled sewage outfalls, other potential inputs, such as urban rivers and drains, and surrounding beaches, and used genetic fingerprints from E. coli and enterococci communities, fecal markers and 454 pyrosequencing to track contamination sources.Two other treated effluent discharges did not appear to influence sites other than those directly adjacent.Generally, connectivity between the sites was observed within distinct geographical locations and it appeared that most of the bacterial contamination on Darwin beaches was confined to local sources.

View Article: PubMed Central - PubMed

Affiliation: Research Institute for the Environment and Livelihoods, Charles Darwin University, Casuarina, Northern Territory, Australia.

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Related in: MedlinePlus

Network analysis of OTUs (97% similarity) shared between the outfalls and beaches for the Firmicutes (A) and Gammaproteobacteria (B). OTU node and edges have been highlighted in blue if they were detected in at least two outfalls and had an abundance of at least 100 in one of the sites. Beach sites are colored green and outfall sites are red. The classification of each OTU to the lowest confident level is written above the node. OTU, operational taxonomic units.
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fig08: Network analysis of OTUs (97% similarity) shared between the outfalls and beaches for the Firmicutes (A) and Gammaproteobacteria (B). OTU node and edges have been highlighted in blue if they were detected in at least two outfalls and had an abundance of at least 100 in one of the sites. Beach sites are colored green and outfall sites are red. The classification of each OTU to the lowest confident level is written above the node. OTU, operational taxonomic units.

Mentions: To more closely examine connections between sites (Figs. 6 and 7; Table 3), a network was drawn that contained only OTUs shared between outfalls and beaches (Fig. 8). Many of the most abundant shared OTUs are typically associated with sewage, such as Clostridiales, Streptococcus, Peptostreptococcaceae, Aeromonas, Enterobacter, and Haemophilus (Scott et al. 2005; McQuaig et al. 2012; McLellan et al. 2013; Newton et al. 2013; Shanks et al., 2013). Again, the Larrakeyah discharge (site 27) appeared to contribute bacteria to surrounding beaches (sites 28 and 29). Similar to the SourceTracker results, Rapid Creek Beach (site 4) contained potential fecal OTUs, and again several sites near Mindil Beach (sites 16, 20 and 23) were linked to fecal OTUs.


Multiple approaches to microbial source tracking in tropical northern Australia.

Neave M, Luter H, Padovan A, Townsend S, Schobben X, Gibb K - Microbiologyopen (2014)

Network analysis of OTUs (97% similarity) shared between the outfalls and beaches for the Firmicutes (A) and Gammaproteobacteria (B). OTU node and edges have been highlighted in blue if they were detected in at least two outfalls and had an abundance of at least 100 in one of the sites. Beach sites are colored green and outfall sites are red. The classification of each OTU to the lowest confident level is written above the node. OTU, operational taxonomic units.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263510&req=5

fig08: Network analysis of OTUs (97% similarity) shared between the outfalls and beaches for the Firmicutes (A) and Gammaproteobacteria (B). OTU node and edges have been highlighted in blue if they were detected in at least two outfalls and had an abundance of at least 100 in one of the sites. Beach sites are colored green and outfall sites are red. The classification of each OTU to the lowest confident level is written above the node. OTU, operational taxonomic units.
Mentions: To more closely examine connections between sites (Figs. 6 and 7; Table 3), a network was drawn that contained only OTUs shared between outfalls and beaches (Fig. 8). Many of the most abundant shared OTUs are typically associated with sewage, such as Clostridiales, Streptococcus, Peptostreptococcaceae, Aeromonas, Enterobacter, and Haemophilus (Scott et al. 2005; McQuaig et al. 2012; McLellan et al. 2013; Newton et al. 2013; Shanks et al., 2013). Again, the Larrakeyah discharge (site 27) appeared to contribute bacteria to surrounding beaches (sites 28 and 29). Similar to the SourceTracker results, Rapid Creek Beach (site 4) contained potential fecal OTUs, and again several sites near Mindil Beach (sites 16, 20 and 23) were linked to fecal OTUs.

Bottom Line: To address this, we sampled sewage outfalls, other potential inputs, such as urban rivers and drains, and surrounding beaches, and used genetic fingerprints from E. coli and enterococci communities, fecal markers and 454 pyrosequencing to track contamination sources.Two other treated effluent discharges did not appear to influence sites other than those directly adjacent.Generally, connectivity between the sites was observed within distinct geographical locations and it appeared that most of the bacterial contamination on Darwin beaches was confined to local sources.

View Article: PubMed Central - PubMed

Affiliation: Research Institute for the Environment and Livelihoods, Charles Darwin University, Casuarina, Northern Territory, Australia.

Show MeSH
Related in: MedlinePlus