Endothelial cells use dynamic actin to facilitate lymphocyte transendothelial migration and maintain the monolayer barrier.
Bottom Line: The actin cytoskeleton of the endothelial cell (EC) is known to facilitate transmigration, but the cellular and molecular mechanisms are not well understood.We found that docking structure formation involves the localization and activation of Arp2/3 complex by WAVE2.Finally, we found that ECs in resting endothelial monolayers use lamellipodial protrusions dependent on WAVE2 to form and maintain contacts and junctions between cells.
Affiliation: Department of Cell Biology and Physiology, Washington University, St. Louis, MO 63110.Show MeSH
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Mentions: Many WAVE2-depleted cells displayed rounded-up and blebbing morphologies, as noted earlier. These cells did not contain either circumferential F-actin or stress fibers (Supplemental Figure S5A, red stars). Therefore, for this analysis, we focused on cells that were in a monolayer and fully spread. We observed that the fluorescence intensity of myosin-II and F-actin in circumferential F-actin fibers in such cells was increased in WAVE2-depleted endothelial monolayers compared with control-cell monolayers (Figure 8, A and B). This finding suggests that increased tension from circumferential actin causes WAVE2-depleted cells to round up.
Affiliation: Department of Cell Biology and Physiology, Washington University, St. Louis, MO 63110.